ErbB4 Monoclonal Antibody (HFR1) | Invitrogen MA1-861 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ErbB4 Monoclonal Antibody (HFR1)

catalog :

MA1-861

quantity :

200 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HFR1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:100; fig 3b	Foth R, Shomroni O, Sigler M, Hörer J, Cleuziou J, Paul T, et al. Screening for potential targets to reduce stenosis in bioprosthetic heart valves. Sci Rep. 2021;11:2464 pubmed publisher
western blot; mouse; loading ...; fig 3f	Huh S, Baek S, Lee K, Whitcomb D, Jo J, Choi S, et al. The reemergence of long-term potentiation in aged Alzheimer's disease mouse model. Sci Rep. 2016;6:29152 pubmed publisher
immunohistochemistry; human	Jerjees D, Alabdullah M, Green A, Alshareeda A, Macmillan R, Ellis I, et al. Prognostic and biological significance of proliferation and HER2 expression in the luminal class of breast cancer. Breast Cancer Res Treat. 2014;145:317-30 pubmed publisher
immunohistochemistry; human	Thorup M, Munk M, Poulsen S, Gaihede M, Nexo E, Sorensen B, et al. Expression of the epidermal growth factor system in human middle ear cholesteatoma. Acta Otolaryngol. 2014;134:124-34 pubmed publisher
immunohistochemistry; human	Paatero I, Lassus H, Junttila T, Kaskinen M, Butzow R, Elenius K. CYT-1 isoform of ErbB4 is an independent prognostic factor in serous ovarian cancer and selectively promotes ovarian cancer cell growth in vitro. Gynecol Oncol. 2013;129:179-87 pubmed publisher
immunohistochemistry; human; 1:100	Hollmen M, Liu P, Kurppa K, Wildiers H, Reinvall I, Vandorpe T, et al. Proteolytic processing of ErbB4 in breast cancer. PLoS ONE. 2012;7:e39413 pubmed publisher
immunocytochemistry; human	Paatero I, Jokilammi A, Heikkinen P, Iljin K, Kallioniemi O, Jones F, et al. Interaction with ErbB4 promotes hypoxia-inducible factor-1? signaling. J Biol Chem. 2012;287:9659-71 pubmed publisher
immunohistochemistry; human; 1:30	Ko E, Park S, Cho E, Kim Y, Hwang J, Lee Y, et al. Cystatin M loss is associated with the losses of estrogen receptor, progesterone receptor, and HER4 in invasive breast cancer. Breast Cancer Res. 2010;12:R100 pubmed publisher
immunohistochemistry; human; 1:10	Gulati S, Ytterhus B, Granli U, Gulati M, Lydersen S, Torp S. Overexpression of c-erbB2 is a negative prognostic factor in anaplastic astrocytomas. Diagn Pathol. 2010;5:18 pubmed publisher
immunohistochemistry; human; 1:40	te Velde E, Franke A, van Hillegersberg R, Elshof S, de Weger R, Borel Rinkes I, et al. HER-family gene amplification and expression in resected pancreatic cancer. Eur J Surg Oncol. 2009;35:1098-104 pubmed publisher
immunohistochemistry; human; 4.0 ug/ml	Naresh A, Thor A, Edgerton S, Torkko K, Kumar R, Jones F. The HER4/4ICD estrogen receptor coactivator and BH3-only protein is an effector of tamoxifen-induced apoptosis. Cancer Res. 2008;68:6387-95 pubmed publisher
immunohistochemistry; human	Kirkegaard T, Naresh A, Sabine V, Tovey S, Edwards J, Dunne B, et al. Expression of tumor necrosis factor alpha converting enzyme in endocrine cancers. Am J Clin Pathol. 2008;129:735-43 pubmed publisher
immunohistochemistry; human	Kassouf W, Black P, Tuziak T, Bondaruk J, Lee S, Brown G, et al. Distinctive expression pattern of ErbB family receptors signifies an aggressive variant of bladder cancer. J Urol. 2008;179:353-8 pubmed
immunohistochemistry; human; 1:10	Torp S, Gulati S, Johannessen E, Dalen A. Coexpression of c-erbB 1-4 receptor proteins in human glioblastomas. An immunohistochemical study. J Exp Clin Cancer Res. 2007;26:353-9 pubmed
immunocytochemistry; human	Sundvall M, Peri L, Määttä J, Tvorogov D, Paatero I, Savisalo M, et al. Differential nuclear localization and kinase activity of alternative ErbB4 intracellular domains. Oncogene. 2007;26:6905-14 pubmed
immunohistochemistry; human; 1:40	Giuliani R, Durbecq V, Di Leo A, Paesmans M, Larsimont D, Leroy J, et al. Phosphorylated HER-2 tyrosine kinase and Her-2/neu gene amplification as predictive factors of response to trastuzumab in patients with HER-2 overexpressing metastatic breast cancer (MBC). Eur J Cancer. 2007;43:725-35 pubmed
immunohistochemistry; human	Ejskjaer K, Sørensen B, Poulsen S, Forman A, Nexø E, Mogensen O. Expression of the epidermal growth factor system in endometrioid endometrial cancer. Gynecol Oncol. 2007;104:158-67 pubmed
immunohistochemistry; human; 1:100	Kountourakis P, Pavlakis K, Psyrri A, Rontogianni D, Xiros N, Patsouris E, et al. Prognostic significance of HER3 and HER4 protein expression in colorectal adenocarcinomas. BMC Cancer. 2006;6:46 pubmed
immunohistochemistry; human; 6:4	Rakha E, Putti T, Abd El Rehim D, Paish C, Green A, Powe D, et al. Morphological and immunophenotypic analysis of breast carcinomas with basal and myoepithelial differentiation. J Pathol. 2006;208:495-506 pubmed
immunohistochemistry; human; 1:50	Edwards J, Traynor P, Munro A, Pirret C, Dunne B, Bartlett J. The role of HER1-HER4 and EGFRvIII in hormone-refractory prostate cancer. Clin Cancer Res. 2006;12:123-30 pubmed
immunohistochemistry; human; 1:50	Leibl S, Bodo K, Gogg Kammerer M, Hrzenjak A, Petru E, Winter R, et al. Ovarian granulosa cell tumors frequently express EGFR (Her-1), Her-3, and Her-4: An immunohistochemical study. Gynecol Oncol. 2006;101:18-23 pubmed
immunohistochemistry; human; 1:40	Lee C, Shrieve D, Zempolich K, Lee R, Hammond E, Handrahan D, et al. Correlation between human epidermal growth factor receptor family (EGFR, HER2, HER3, HER4), phosphorylated Akt (P-Akt), and clinical outcomes after radiation therapy in carcinoma of the cervix. Gynecol Oncol. 2005;99:415-21 pubmed
immunohistochemistry; human; 1:100, 1:400	Ejskjaer K, Sørensen B, Poulsen S, Mogensen O, Forman A, Nexø E. Expression of the epidermal growth factor system in human endometrium during the menstrual cycle. Mol Hum Reprod. 2005;11:543-51 pubmed
immunohistochemistry; human; 1:160	Prentice L, Shadeo A, Lestou V, Miller M, DeLeeuw R, Makretsov N, et al. NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis. Oncogene. 2005;24:7281-9 pubmed
immunohistochemistry; human; 1:200	Hughes D, Thomas D, Giordano T, McDonagh K, Baker L. Essential erbB family phosphorylation in osteosarcoma as a target for CI-1033 inhibition. Pediatr Blood Cancer. 2006;46:614-23 pubmed
immunohistochemistry; human; 1:20	Fountzilas G, Pectasides D, Kalogera Fountzila A, Skarlos D, Kalofonos H, Papadimitriou C, et al. Paclitaxel and carboplatin as first-line chemotherapy combined with gefitinib (IRESSA) in patients with advanced breast cancer: a phase I/II study conducted by the Hellenic Cooperative Oncology Group. Breast Cancer Res Treat. 2005;92:1-9 pubmed
immunohistochemistry; human	Abd El Rehim D, Ball G, Pinder S, Rakha E, Paish C, Robertson J, et al. High-throughput protein expression analysis using tissue microarray technology of a large well-characterised series identifies biologically distinct classes of breast cancer confirming recent cDNA expression analyses. Int J Cancer. 2005;116:340-50 pubmed
immunohistochemistry; human; 1:160	Wiseman S, Makretsov N, Nielsen T, Gilks B, Yorida E, Cheang M, et al. Coexpression of the type 1 growth factor receptor family members HER-1, HER-2, and HER-3 has a synergistic negative prognostic effect on breast carcinoma survival. Cancer. 2005;103:1770-7 pubmed
western blot; human	Caron R, Yacoub A, Zhu X, Mitchell C, Han S, Sasazuki T, et al. H-RAS V12-induced radioresistance in HCT116 colon carcinoma cells is heregulin dependent. Mol Cancer Ther. 2005;4:243-55 pubmed
	Kundumani Sridharan V, Subramani J, Owens C, Das K. Nrg1β Released in Remote Ischemic Preconditioning Improves Myocardial Perfusion and Decreases Ischemia/Reperfusion Injury via ErbB2-Mediated Rescue of Endothelial Nitric Oxide Synthase and Abrogation of Trx2 Autophagy. Arterioscler Thromb Vasc Biol. 2021;41:2293-2314 pubmed publisher
	Abd El Rehim D, Pinder S, Paish C, Bell J, Rampaul R, Blamey R, et al. Expression and co-expression of the members of the epidermal growth factor receptor (EGFR) family in invasive breast carcinoma. Br J Cancer. 2004;91:1532-42 pubmed
	Mano M, Awada A, Minisini A, Durbecq V, Di Leo A, Piccart M. Exquisite antitumour response to trastuzumab in a patient with no evidence of Ras-Raf-MAPK and PI3K-Akt pathways activation. Breast. 2004;13:347-9 pubmed
	Adélaïde J, Huang H, Murati A, Alsop A, Orsetti B, Mozziconacci M, et al. A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene. Genes Chromosomes Cancer. 2003;37:333-45 pubmed
	Yu W, Woessner J, McNeish J, Stamenkovic I. CD44 anchors the assembly of matrilysin/MMP-7 with heparin-binding epidermal growth factor precursor and ErbB4 and regulates female reproductive organ remodeling. Genes Dev. 2002;16:307-23 pubmed
	Lammering G, Valerie K, Lin P, Mikkelsen R, Contessa J, Feden J, et al. Radiosensitization of malignant glioma cells through overexpression of dominant-negative epidermal growth factor receptor. Clin Cancer Res. 2001;7:682-90 pubmed

product information

Product Type :

Antibody

Product Name :

ErbB4 Monoclonal Antibody (HFR1)

Catalog # :

MA1-861

Quantity :

200 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 0.5-2 µg / test, Immunocytochemistry: 1:50, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:500

Species :

Human, Mouse

Clone :

HFR1

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

ErbB 4 (Her-4, c-erbB-4, v-erbB-4) is a member of the class I receptor kinase family. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

Immunogen :

Synthetic Peptide: C R(1250) S T L Q H P D Y L Q E Y S T(1264)

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5-2 µg / test, Immunocytochemistry: 1:50, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:500

Aliases :

4ICD; ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; erb-b2 receptor tyrosine kinase 4; Erbb4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; Her4; human epidermal growth factor receptor 4; Mer4; OTTHUMP00000209432; p180erbB4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-4; s80HER4; Tyro-2; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v-erb-b2 avian erythroblastic leukemia viral oncogene 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

more info or order :

Invitrogen product webpage