gamma Tubulin Monoclonal Antibody (4D11) | Invitrogen MA1-850 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

gamma Tubulin Monoclonal Antibody (4D11)

catalog :

MA1-850

quantity :

100 µg

price :

345 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D11

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 8a	Abu Taha I, Heijman J, Hippe H, Wolf N, El Armouche A, Nikolaev V, et al. Nucleoside Diphosphate Kinase-C Suppresses cAMP Formation in Human Heart Failure. Circulation. 2017;135:881-897 pubmed publisher
western blot; human; loading ...; fig 1c	Maina P, Shao P, Liu Q, Fazli L, Tyler S, Nasir M, et al. c-MYC drives histone demethylase PHF8 during neuroendocrine differentiation and in castration-resistant prostate cancer. Oncotarget. 2016;7:75585-75602 pubmed publisher
immunocytochemistry; human; fig 2	Acevedo Acevedo S, Crone W. Substrate stiffness effect and chromosome missegregation in hIPS cells. J Negat Results Biomed. 2015;14:22 pubmed publisher
western blot; mouse	Patrick P, Price T, Diogo A, Sheibani N, Banks W, Shah G. Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes. J Endocrinol Diabetes. 2015;2: pubmed
western blot; human	Fuentes E, Forero Doria O, AlarcÃ³n M, Palomo I. Inhibitory effects of Cyperus digitatus extract on human platelet function in vitro. Platelets. 2015;26:764-70 pubmed publisher
western blot; human	Fuentes E, AlarcÃ³n M, Fuentes M, Carrasco G, Palomo I. A novel role of Eruca sativa Mill. (rocket) extract: antiplatelet (NF-ÎºB inhibition) and antithrombotic activities. Nutrients. 2014;6:5839-52 pubmed publisher
western blot; human	Yan S, Wang C, Wei W, Gaertig M, Lai L, Li S, et al. TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain. Hum Mol Genet. 2014;23:2678-93 pubmed publisher
immunohistochemistry; mouse	Smith W, Liu Z, Liang Y, Masuda N, Swing D, Jenkins N, et al. Synphilin-1 attenuates neuronal degeneration in the A53T alpha-synuclein transgenic mouse model. Hum Mol Genet. 2010;19:2087-98 pubmed publisher
immunocytochemistry; human western blot; human	Seitzer U, Gerber S, Beyer D, Dobschanski J, Kullmann B, Haller D, et al. Schizonts of Theileria annulata interact with the microtubuli network of their host cell via the membrane protein TaSP. Parasitol Res. 2010;106:1085-102 pubmed publisher
	Liu Q, Borcherding N, Shao P, Maina P, Zhang W, Qi H. Contribution of synergism between PHF8 and HER2 signalling to breast cancer development and drug resistance. EBioMedicine. 2020;51:102612 pubmed publisher
	Liu Q, Borcherding N, Shao P, Cao H, Zhang W, Qi H. Identification of novel TGF-β regulated genes with pro-migratory roles. Biochim Biophys Acta Mol Basis Dis. 2019;1865:165537 pubmed publisher
	Groenendyk J, Paskevicius T, Urra H, Viricel C, Wang K, Barakat K, et al. Cyclosporine A binding to COX-2 reveals a novel signaling pathway that activates the IRE1α unfolded protein response sensor. Sci Rep. 2018;8:16678 pubmed publisher
	Ohmine S, Salisbury J, Ingle J, Pettinato G, Haddox C, Haddad T, et al. Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells. Oncol Rep. 2018;39:1725-1730 pubmed publisher
	Wang W, Liu W, Durnaoglu S, Lee S, Lian J, Lehner R, et al. Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis. Sci Rep. 2017;7:5941 pubmed publisher
	Siriwardana N, Meyer R, Panchenko M. The novel function of JADE1S in cytokinesis of epithelial cells. Cell Cycle. 2015;14:2821-34 pubmed publisher
	Zhou Z, Patel M, Ng N, Hsieh M, Orth A, Walker J, et al. Identification of synthetic lethality of PRKDC in MYC-dependent human cancers by pooled shRNA screening. BMC Cancer. 2014;14:944 pubmed publisher
	Messaed C, Akoury E, Djuric U, Zeng J, Saleh M, Gilbert L, et al. NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center. J Biol Chem. 2011;286:43313-23 pubmed publisher

image

image 1 :

Immunofluorescent analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (Pierce Product# MA1-850, Clone 4D11) (shown in green) in HeLa (A) and U2-OS cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Pierce Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product# MA1-850), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Pierce Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Pierce Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

image 2 :

Western blot analysis of ?-Tubulin was performed by loading 50ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product # MA1-850, Clone 4D11) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Pierce Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Pierce Product #34075).

product information

Product Type :

Antibody

Product Name :

gamma Tubulin Monoclonal Antibody (4D11)

Catalog # :

MA1-850

Quantity :

100 µg

Price :

345 USD

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry: 1:200, Immunoprecipitation: Assay dependent, Western Blot: 1:1000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

4D11

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

Immunogen :

Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry: 1:200, Immunoprecipitation: Assay dependent, Western Blot: 1:1000

Aliases :

1500010O08Rik; AI451582; AI503389; AI504772; CDCBM4; centrosome marker; gamma tubulin; gamma-1-tubulin; Gamma-2-tubulin; gamma-tubulin; gamma-tubulin complex component 1; GCP-1; similar to tubulin, gamma 1; TUBG; Tubg1; tubg1.L; TUBG2; TUBGCP1; Tubgl; tubulin gamma 1; tubulin gamma 1 L homeolog; tubulin gamma 2; Tubulin gamma-1 chain; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide; Unknown (protein for MGC:133882); XELAEV_18002398mg; xgam; zgc:55894

more info or order :

Invitrogen product webpage