PLK1 Monoclonal Antibody (13E8) | Invitrogen MA1-848 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PLK1 Monoclonal Antibody (13E8)

catalog :

MA1-848

quantity :

100 µg

price :

US 470.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13E8

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
ELISA; human; 1 ug/ml	Mita Y, Noguchi Yachide T, Ishikawa M, Hashimoto Y. Small-molecular, non-peptide, non-ATP-competitive polo-like kinase 1 (Plk1) inhibitors with a terphenyl skeleton. Bioorg Med Chem. 2013;21:608-17 pubmed publisher
	Arroyo M, Cañuelo A, Calahorra J, Hastert F, Sanchez A, Clarke D, et al. Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1. FEBS J. 2020;287:4933-4951 pubmed publisher

image

image 1 :

Western blot analysis of PLK1 was performed by loading 25ug of untreated or Nocodazole (100nM, 48 hours) treated U2OS lysate from non-targeting control (Thermo Product# D-001810-10) or PLK1 SMART pool siRNA (Thermo Product# L-003290-00-0005) transfected U2OS cells onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody (Pierce Product# MA1-848) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Pierce Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Pierce Product #34075).

image 2 :

Immunofluorescent analysis of PLK1 using PLK1 Monoclonal antibody (13E8) (Product# MA1-848) shows staining in HeLa cells. PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 (Product# MA1-848) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

PLK1 Monoclonal Antibody (13E8)

Catalog # :

MA1-848

Quantity :

100 µg

Price :

US 470.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 3 µg/mL, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Clone :

13E8

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

Immunogen :

Recombinant protein containing residues 300-400 of human PLK1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 3 µg/mL, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

cell cycle regulated protein kinase; Plk; PLK 1; Plk1; PLK-1; plk1.S; plx1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; polo like kinase 1 S homeolog; polo-like kinase; polo-like kinase 1; polo-like kinase homolog; polo-like protein kinase; serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; Unknown (protein for MGC:133927); XELAEV_18047692mg

more info or order :

Invitrogen product webpage