PRC1 Monoclonal Antibody (16F2) | Invitrogen MA1-846 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PRC1 Monoclonal Antibody (16F2)

catalog :

MA1-846

quantity :

100 µg

price :

US 430.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

16F2

reactivity :

human, mouse, rat, dogs

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; dogs; 1:200; loading ...; fig 2c	BernabÃ© Rubio M, AndrÃ©s G, Casares Arias J, FernÃ¡ndez Barrera J, Rangel L, Reglero Real N, et al. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells. J Cell Biol. 2016;214:259-73 pubmed publisher

image

image 1 :

Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (Product# MA1-846 ) shows staining in A2058 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (Product# MA1-846 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (Product# MA1-846 ) shows staining in Hela Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (Product# MA1-846 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 3 :

Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (Product# MA1-846 ) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (Product# MA1-846 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

PRC1 Monoclonal Antibody (16F2)

Catalog # :

MA1-846

Quantity :

100 µg

Price :

US 430.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1-2 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

16F2

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly.

Immunogen :

Recombinant PRC1 protein.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1-2 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Aliases :

anaphase spindle elongation 1 homolog; ASE1; carboxypeptidase C PRC1; Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; CPY1; D7Ertd348e; LBC1; MGC1671; MGC3669; prc1; protein regulating cytokinesis 1; protein regulator of cytokinesis 1; YMR297W

more info or order :

Invitrogen product webpage