SRC3 Monoclonal Antibody (AX15.3) | Invitrogen MA1-845 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SRC3 Monoclonal Antibody (AX15.3)

catalog :

MA1-845

quantity :

100 µg

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AX15.3

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human	Hao L, Rizzo P, Osipo C, Pannuti A, Wyatt D, Cheung L, et al. Notch-1 activates estrogen receptor-alpha-dependent transcription via IKKalpha in breast cancer cells. Oncogene. 2010;29:201-13 pubmed publisher
western blot; human	Naughton C, MacLeod K, Kuske B, Clarke R, Cameron D, Langdon S. Progressive loss of estrogen receptor alpha cofactor recruitment in endocrine resistance. Mol Endocrinol. 2007;21:2615-26 pubmed
	Park K, Krishnan V, O Malley B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 pubmed
	Oda Y, Sihlbom C, Chalkley R, Huang L, Rachez C, Chang C, et al. Two distinct coactivators, DRIP/mediator and SRC/p160, are differentially involved in vitamin D receptor transactivation during keratinocyte differentiation. Mol Endocrinol. 2003;17:2329-39 pubmed
	Azorsa D, Meltzer P. Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1. Hybridoma. 1999;18:281-7 pubmed

image

image 1 :

Western blot of AIB1 on MCF-7 cell extract using MA1-845.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a SRC3 monoclonal antibody (Product # MA1-845) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting

product information

Product Type :

Antibody

Product Name :

SRC3 Monoclonal Antibody (AX15.3)

Catalog # :

MA1-845

Quantity :

100 µg

Price :

US 450.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

ChIP Assay: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL

Species :

Human

Clone :

AX15.3

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

AIB1-AC3 recognizes AIB1, a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160kDa. The AIB1 gene has been mapped to region 20q12 and has been shown to be amplified in breast tumors and pancreatic cancer cell lines. AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.

Immunogen :

GST fusion protein encoding amino acid residues 605-1294 of human AIB1.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL

Aliases :

2010305B15Rik; Actr; AIB1; AIB-1; amplified in breast cancer 1 protein; amplified in breast cancer-1 protein homolog; AW321064; BHLHE42; CAGH16; CBP-interacting protein; class E basic helix-loop-helix protein 42; CTG26; KAT13B; MGC141848; NCOA3; NCoA-3; Nuclear receptor coactivator 3; OTTHUMP00000031716; p/Cip; pCip; Rac3; RAC-3; Receptor-associated coactivator 3; RP5-1049G16.3; Src3; SRC-3; steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; Tram1; Tram-1

more info or order :

Invitrogen product webpage