Alix Monoclonal Antibody (3A9) | Invitrogen MA1-83977 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Alix Monoclonal Antibody (3A9)

catalog :

MA1-83977

quantity :

100 µg

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A9

reactivity :

human, mouse, rat, Xenopus laevis

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 6a, 7a western blot; human; 1:500; loading ...; fig 4d	Ajasin D, Rao V, Wu X, Ramasamy S, Pujato M, Ruiz A, et al. CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release. elife. 2019;8: pubmed publisher
western blot; human; loading ...; fig 1a	Ortega F, Roefs M, De Miguel Pérez D, Kooijmans S, de Jong O, Sluijter J, et al. Interfering with endolysosomal trafficking enhances release of bioactive exosomes. Nanomedicine. 2019;:102014 pubmed publisher
	Roefs M, Bauz xe1 Martinez J, van de Wakker S, Qin J, Olijve W, Tuinte R, et al. Cardiac progenitor cell-derived extracellular vesicles promote angiogenesis through both associated- and co-isolated proteins. Commun Biol. 2023;6:800 pubmed publisher
	Laso Garc xed a F, Piniella D, G xf3 mez de Frutos M, Casado Fern xe1 ndez L, P xe9 rez Mato M, Alonso L xf3 pez E, et al. Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage. Front Cell Neurosci. 2022;16:1058546 pubmed publisher
	Peng H, Hou M, Wu Z, Wang J, Zhou M, Zhuang X, et al. Plasma exosomal miR-122 regulates the efficacy of metformin via AMPK in type 2 diabetes and hepatocellular carcinoma. Heliyon. 2022;8:e11503 pubmed publisher
	Shukla S, Larsen K, Ou C, Rose K, Hurley J. In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair. Proc Natl Acad Sci U S A. 2022;119:e2205590119 pubmed publisher
	Le Gall L, Duddy W, Martinat C, Mariot V, Connolly O, Milla V, et al. Muscle cells of sporadic amyotrophic lateral sclerosis patients secrete neurotoxic vesicles. J Cachexia Sarcopenia Muscle. 2022;13:1385-1402 pubmed publisher
	Kapustin A, Davey P, Longmire D, Matthews C, Linnane E, Rustogi N, et al. Antisense oligonucleotide activity in tumour cells is influenced by intracellular LBPA distribution and extracellular vesicle recycling. Commun Biol. 2021;4:1241 pubmed publisher
	Elsayed R, Elashiry M, Liu Y, El Awady A, Hamrick M, Cutler C. Porphyromonas gingivalis Provokes Exosome Secretion and Paracrine Immune Senescence in Bystander Dendritic Cells. Front Cell Infect Microbiol. 2021;11:669989 pubmed publisher
	Mitchell M, Ben Dov I, Liu C, Ye K, Chow K, Kramer Y, et al. Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs. J Extracell Vesicles. 2021;10:e12110 pubmed publisher
	Tolomeo A, Castagliuolo I, Piccoli M, Grassi M, Magarotto F, De Lazzari G, et al. Extracellular Vesicles Secreted by Mesenchymal Stromal Cells Exert Opposite Effects to Their Cells of Origin in Murine Sodium Dextran Sulfate-Induced Colitis. Front Immunol. 2021;12:627605 pubmed publisher
	Li C, Wang C, Zhang Y, Alsrouji O, Chebl A, Ding G, et al. Cerebral endothelial cell-derived small extracellular vesicles enhance neurovascular function and neurological recovery in rat acute ischemic stroke models of mechanical thrombectomy and embolic stroke treatment with tPA. J Cereb Blood Flow Metab. 2021;:271678X21992980 pubmed publisher
	Zhu X, Qin X, Wang X, Wang Y, Cao W, Zhang J, et al. Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells. Int J Mol Med. 2020;46:2115-2125 pubmed publisher
	Eriksson I, Wäster P, Ollinger K. Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins. Cell Death Dis. 2020;11:370 pubmed publisher
	de Jong O, Murphy D, Mäger I, Willms E, Garcia Guerra A, Gitz Francois J, et al. A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. Nat Commun. 2020;11:1113 pubmed publisher
	Venturini A, Passalacqua M, Pelassa S, Pastorino F, Tedesco M, Cortese K, et al. Exosomes From Astrocyte Processes: Signaling to Neurons. Front Pharmacol. 2019;10:1452 pubmed publisher
	Trubetckaia O, Lane A, Qian L, Zhou P, Lane D. Alpha-synuclein is strategically positioned for afferent modulation of midbrain dopamine neurons and is essential for cocaine preference. Commun Biol. 2019;2:418 pubmed publisher
	Srinivasan S, Duval M, Kaimal V, Cuff C, Clarke S. Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development. J Extracell Vesicles. 2019;8:1684425 pubmed publisher
	Corciulo S, Nicoletti M, Mastrofrancesco L, Milano S, Mastrodonato M, Carmosino M, et al. AQP1-Containing Exosomes in Peritoneal Dialysis Effluent As Biomarker of Dialysis Efficiency. Cells. 2019;8: pubmed publisher
	Thepparit C, Khongwichit S, Ketsuwan K, Libsittikul S, Auewarakul P, Smith D. Dengue virus requires apoptosis linked gene-2-interacting protein X (ALIX) for viral propagation. Virus Res. 2019;261:65-71 pubmed publisher
	Logozzi M, Mizzoni D, Angelini D, Di Raimo R, Falchi M, Battistini L, et al. Microenvironmental pH and Exosome Levels Interplay in Human Cancer Cell Lines of Different Histotypes. Cancers (Basel). 2018;10: pubmed publisher
	Boussadia Z, Lamberti J, Mattei F, Pizzi E, Puglisi R, Zanetti C, et al. Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules. J Exp Clin Cancer Res. 2018;37:245 pubmed publisher
	Kamranvar S, Gupta D, Huang Y, Gupta R, Johansson S. Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody. Oncotarget. 2016;7:30820-30 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Alix Monoclonal Antibody (3A9)

Catalog # :

MA1-83977

Quantity :

100 µg

Price :

US 496.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat, Xenopus laevis

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500

Species :

Human, Mouse, Rat, Xenopus laevis

Clone :

3A9

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.

Immunogen :

Alix fusion protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500

Aliases :

AI480591; AIP1; ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; Alg2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein X; Alix; apoptosis-linked gene 2-interacting protein X; AW544830; C76364; dopamine receptor interacting protein 4; DRIP4; E2F1-inducible protein; Eig2; Hp95; KIAA1375; MGC17003; mKIAA1375; PDCD6-interacting protein; Pdcd6ip; pdcd6ip.L; programmed cell death 6 interacting protein; programmed cell death 6 interacting protein L homeolog; programmed cell death 6-interacting protein; putative signal tranduction protein Xp95; RGD1561176; Signal transduction protein Xp95; XELAEV_18031317mg; xp95

more info or order :

Invitrogen product webpage