PGP9.5 Monoclonal Antibody (31A3) | Invitrogen MA1-83428 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PGP9.5 Monoclonal Antibody (31A3)

catalog :

MA1-83428

quantity :

200 µg

price :

US 546.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

31A3

reactivity :

human, mouse, rat, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:2000; loading ...; fig 8	Singh M, Kasna S, Roy S, Aldosary S, Saeedan A, Ansari M, et al. Repurposing mechanistic insight of PDE-5 inhibitor in cancer chemoprevention through mitochondrial-oxidative stress intervention and blockade of DuCLOX signalling. BMC Cancer. 2019;19:996 pubmed publisher
western blot; rat; 1:2000; loading ...; fig 5	Yadav S, Tiwari V, Singh M, Yadav R, Roy S, Devi U, et al. Comparative efficacy of alpha-linolenic acid and gamma-linolenic acid to attenuate valproic acid-induced autism-like features. J Physiol Biochem. 2017;73:187-198 pubmed publisher
western blot; rat; 1:2000; fig 3	Rani A, Roy S, Singh M, Devi U, Yadav R, Gautam S, et al. ?-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats. Inflammopharmacology. 2016;24:277-286 pubmed
western blot; rat; 1:2000; loading ...; fig 4	Manral C, Roy S, Singh M, Gautam S, Yadav R, Rawat J, et al. Effect of ?-sitosterol against methyl nitrosourea-induced mammary gland carcinoma in albino rats. BMC Complement Altern Med. 2016;16:260 pubmed publisher
	De Guilhem De Lataillade A, Caillaud M, Oullier T, Naveilhan P, Pellegrini C, Tolosa E, et al. LRRK2 expression in normal and pathologic human gut and in rodent enteric neural cell lines. J Neurochem. 2023;164:193-209 pubmed publisher
	Caillaud M, Le Dr xe9 an M, De Guilhem De Lataillade A, Le Berre Scoul C, Montnach J, Nedellec S, et al. A functional network of highly pure enteric neurons in a dish. Front Neurosci. 2022;16:1062253 pubmed publisher
	Emmi A, Stocco E, Boscolo Berto R, Contran M, Belluzzi E, Favero M, et al. Infrapatellar Fat Pad-Synovial Membrane Anatomo-Fuctional Unit: Microscopic Basis for Piezo1/2 Mechanosensors Involvement in Osteoarthritis Pain. Front Cell Dev Biol. 2022;10:886604 pubmed publisher
	Lassoz xe9 S, De Guilhem De Lataillade A, Oullier T, Neunslist M, Leclair Visonneau L, Derkinderen P, et al. Comparison of commercially available antibodies for the detection of phosphorylated alpha-synuclein in primary culture of ENS. Neurogastroenterol Motil. 2022;34:e14354 pubmed publisher
	Bodin R, Paill xe9 V, Oullier T, Durand T, Aubert P, Le Berre Scoul C, et al. The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons. J Biol Chem. 2021;297:101300 pubmed publisher
	Singh M, Devi U, Roy S, Gupta P, Kaithwas G. Chemical activation of prolyl hydroxylase-2 by BBAP-1 down regulates hypoxia inducible factor-1α and fatty acid synthase for mammary gland chemoprevention. RSC Adv. 2018;8:12848-12860 pubmed publisher
	Roy S, Singh M, Rawat A, Kumar D, Kaithwas G. Mitochondrial apoptosis and curtailment of hypoxia-inducible factor-1α/fatty acid synthase: A dual edge perspective of gamma linolenic acid in ER+ mammary gland cancer. Cell Biochem Funct. 2020;38:591-603 pubmed publisher
	Devi U, Singh M, Roy S, Gupta P, Ansari M, Saeedan A, et al. Activation of prolyl hydroxylase-2 for stabilization of mitochondrial stress along with simultaneous downregulation of HIF-1α/FASN in ER + breast cancer subtype. Cell Biochem Funct. 2019;37:216-227 pubmed publisher
	Roy S, Rawat A, Sammi S, Devi U, Singh M, Gautam S, et al. Alpha-linolenic acid stabilizes HIF-1 ? and downregulates FASN to promote mitochondrial apoptosis for mammary gland chemoprevention. Oncotarget. 2017;8:70049-70071 pubmed publisher

product information

Product Type :

Antibody

Product Name :

PGP9.5 Monoclonal Antibody (31A3)

Catalog # :

MA1-83428

Quantity :

200 µg

Price :

US 546.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit, Rat

Applications :

ELISA: 1:500-1:2,000, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:400, Western Blot: 1 µg/mL

Species :

Human, Mouse, Rabbit, Rat

Clone :

31A3

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

Immunogen :

Native protein, from brain.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:500-1:2,000, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:400, Western Blot: 1 µg/mL

Aliases :

AW822034; C88048; epididymis luminal protein 117; epididymis secretory protein Li 53; gad; gracile axonal dystrophy; HEL-117; HEL-S-53; MGC133697 protein; NDGOA; neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PARK5; PGP 9.5; PGP9.5; PGP95; protein gene product 9.5; R75593; ubiquitin carboxyl-terminal esterase; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase 1; ubiquitin C-terminal hydrolase L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCHL; UCHL1; UCH-L1; UCHL-1

more info or order :

Invitrogen product webpage