CD59 Monoclonal Antibody (MEM-43) | Invitrogen MA1-82206 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD59 Monoclonal Antibody (MEM-43)

catalog :

MA1-82206

quantity :

1 mg

price :

US 1158.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MEM-43

reactivity :

human, rhesus macaque

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s2	Puhka M, Nordberg M, Valkonen S, Rannikko A, Kallioniemi O, Siljander P, et al. KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine. Eur J Pharm Sci. 2017;98:30-39 pubmed publisher
immunocytochemistry; human; fig 3	Sartain S, Turner N, Moake J. TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells. J Immunol. 2016;196:832-45 pubmed publisher
immunoprecipitation; human; loading ...; fig 3a	LaRocca T, Stivison E, Mal Sarkar T, Hooven T, Hod E, Spitalnik S, et al. CD59 signaling and membrane pores drive Syk-dependent erythrocyte necroptosis. Cell Death Dis. 2015;6:e1773 pubmed publisher
flow cytometry; rhesus macaque; fig 1	Byrnes C, Terry Lee Y, Donahue R, Miller J. Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes. Cytometry A. 2012;81:165-8 pubmed publisher
immunocytochemistry; human	Barcena A, Muench M, Kapidzic M, Gormley M, Goldfien G, Fisher S. Human placenta and chorion: potential additional sources of hematopoietic stem cells for transplantation. Transfusion. 2011;51 Suppl 4:94S-105S pubmed publisher
flow cytometry; human; fig 2	Borowitz M, Craig F, DiGiuseppe J, Illingworth A, Rosse W, Sutherland D, et al. Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry. Cytometry B Clin Cytom. 2010;78:211-30 pubmed publisher
flow cytometry; human	Willcocks L, Lyons P, Clatworthy M, Robinson J, Yang W, Newland S, et al. Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake. J Exp Med. 2008;205:1573-82 pubmed publisher
flow cytometry; human	Williams M, Densmore J, Pawluczkowycz A, Beum P, Kennedy A, Lindorfer M, et al. Thrice-weekly low-dose rituximab decreases CD20 loss via shaving and promotes enhanced targeting in chronic lymphocytic leukemia. J Immunol. 2006;177:7435-43 pubmed
flow cytometry; human	Zucchetto A, Sonego P, Degan M, Bomben R, Dal Bo M, Russo S, et al. Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling. J Cell Physiol. 2005;204:113-23 pubmed
flow cytometry; human	Thomason R, Papiez J, Lee R, Szczarkowski W. Identification of unsuspected PNH-type cells in flow cytometric immunophenotypic analysis of peripheral blood and bone marrow. Am J Clin Pathol. 2004;122:128-34 pubmed
flow cytometry; human	Suskind D, Muench M. Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cells. J Hepatol. 2004;40:261-8 pubmed
immunohistochemistry; human	Fierro M, Savoia P, Quaglino P, Novelli M, Barberis M, Bernengo M. Systemic therapy with cyclophosphamide and anti-CD20 antibody (rituximab) in relapsed primary cutaneous B-cell lymphoma: a report of 7 cases. J Am Acad Dermatol. 2003;49:281-7 pubmed
immunoprecipitation; human; fig 1	Solomon K, Rudd C, Finberg R. The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes. Proc Natl Acad Sci U S A. 1996;93:6053-8 pubmed
western blot; human; fig 4	Koski C, Estep A, Sawant Mane S, Shin M, Highbarger L, Hansch G. Complement regulatory molecules on human myelin and glial cells: differential expression affects the deposition of activated complement proteins. J Neurochem. 1996;66:303-12 pubmed
immunoprecipitation; human	Tseng J, Hall S, Howard T, Ware R. Phenotypic and functional analysis of lymphocytes in paroxysmal nocturnal hemoglobinuria. Am J Hematol. 1995;50:244-53 pubmed
flow cytometry; human	Philbrick W, Palfree R, Maher S, Bridgett M, Sirlin S, Bothwell A. The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. Eur J Immunol. 1990;20:87-92 pubmed
	Wunderley L, Zhang L, Yarwood R, Qin W, Lowe M, Woodman P. Endosomal recycling tubule scission and integrin recycling involve the membrane curvature-supporting protein LITAF. J Cell Sci. 2021;134: pubmed publisher
	Tan L, Toops K, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2016;113:8789-94 pubmed publisher
	Liew M, Farley M, Andreasen J, Parker C, Wittwer C. Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab. Cytometry B Clin Cytom. 2015;88:261-9 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD59 Monoclonal Antibody (MEM-43)

Catalog # :

MA1-82206

Quantity :

1 mg

Price :

US 1158.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Flow Cytometry: 1:5-1:10, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

MEM-43

Isotype :

IgG2a

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). CD59 is the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 also associates with C5b-8 complex and counteracts appropriate formation of cytolytic pore within the plasma membrane. Further, CD59 is a low-affinity ligand of human CD2, causes T cell costimulation, and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. Mutations in this gene cause CD59 deficiency, a disease involving hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants of CD59 have been identified.

Immunogen :

Thymocytes and T lymphocytes.

Format :

Liquid

Applications w/Dilutions :

Aliases :

16.3A5; 1F5; 1F5 antigen; 20 kDa homologous restriction factor; AA987121; Cb59b molecule; CD59; CD59 antigen; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule; CD59 molecule (CD59 blood group); CD59 molecule, complement regulatory protein; Cd59a; CD59a antigen; CD59A glycoprotein; Cd59b; CD59b antigen; CD59B glycoprotein; CD59b moleucle, complement regulatory protein; complement inhibitor CD59; complement regulatory protein; EJ16; EJ30; EL32; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MACIP; MAC-IP; MEM43; MEM43 antigen; membrane attack complex (MAC) inhibition factor; membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20; protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein

more info or order :

Invitrogen product webpage