CD11b/c Monoclonal Antibody (OX-42) | Invitrogen MA1-81606 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD11b/c Monoclonal Antibody (OX-42)

catalog :

MA1-81606

quantity :

100 ug

price :

US 292.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

OX-42

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; rat; loading ...; fig 6e	Tuncel J, Haag S, Holmdahl R. MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis. Eur J Immunol. 2017;47:563-574 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 1	Lee J, Kang S, Yune T. Fluoxetine prevents oligodendrocyte cell death by inhibiting microglia activation after spinal cord injury. J Neurotrauma. 2015;32:633-44 pubmed publisher
flow cytometry; rat	Castelo Branco M, Soares I, Lopes D, Buongusto F, Martinusso C, do Rosario A, et al. Intraperitoneal but not intravenous cryopreserved mesenchymal stromal cells home to the inflamed colon and ameliorate experimental colitis. PLoS ONE. 2012;7:e33360 pubmed publisher
immunocytochemistry; rat; 1:200	Ki K, Park D, Lee S, Kim N, Choi B, Noh G. The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats. Korean J Anesthesiol. 2010;59:403-10 pubmed publisher
immunohistochemistry - free floating section; rat; 1:50; fig 5	Zhang L, Wei W, Li Y, Wang Y. A rat model of mild cognitive impairment associated with vascular factor. Neuropathology. 2011;31:112-21 pubmed publisher
immunohistochemistry; rat; 1:100	Samantaray S, Knaryan V, Guyton M, Matzelle D, Ray S, Banik N. The parkinsonian neurotoxin rotenone activates calpain and caspase-3 leading to motoneuron degeneration in spinal cord of Lewis rats. Neuroscience. 2007;146:741-55 pubmed
immunohistochemistry - frozen section; rat; 1:100; fig 2	Svensson C, Fitzsimmons B, Azizi S, Powell H, Hua X, Yaksh T. Spinal p38beta isoform mediates tissue injury-induced hyperalgesia and spinal sensitization. J Neurochem. 2005;92:1508-20 pubmed
immunohistochemistry - frozen section; rat; 1:100; fig 5	Svensson C, Marsala M, Westerlund A, Calcutt N, Campana W, Freshwater J, et al. Activation of p38 mitogen-activated protein kinase in spinal microglia is a critical link in inflammation-induced spinal pain processing. J Neurochem. 2003;86:1534-44 pubmed
immunohistochemistry; rat; 1:100	Svensson C, Hua X, Protter A, Powell H, Yaksh T. Spinal p38 MAP kinase is necessary for NMDA-induced spinal PGE(2) release and thermal hyperalgesia. Neuroreport. 2003;14:1153-7 pubmed
flow cytometry; rat	Vari F, Lord R, Goto S. LSF-1 may modulate the indirect allorecognition pathway to delay allograft rejection. Transpl Immunol. 2002;10:259-67 pubmed
flow cytometry; rat	Inada T, Taniuchi S, Shingu K, Kobayashi Y, Fujisawa J, Nakao S. Propofol depressed neutrophil hydrogen peroxide production more than midazolam, whereas adhesion molecule expression was minimally affected by both anesthetics in rats with abdominal sepsis. Anesth Analg. 2001;92:437-41 pubmed
immunocytochemistry; rat; 1:100; fig 2	Ray S, Schaecher K, Shields D, Hogan E, Banik N. Combined TUNEL and double immunofluorescent labeling for detection of apoptotic mononuclear phagocytes in autoimmune demyelinating disease. Brain Res Brain Res Protoc. 2000;5:305-11 pubmed
immunohistochemistry; rat; fig 3	Sigurdsson E, Permanne B, Soto C, Wisniewski T, Frangione B. In vivo reversal of amyloid-beta lesions in rat brain. J Neuropathol Exp Neurol. 2000;59:11-7 pubmed
flow cytometry; rat	Lan F, Hayamizu K, Strober S. Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation. Transplantation. 2000;69:649-55 pubmed
flow cytometry; rat; fig 1	Hayamizu K, Lan F, Huie P, Sibley R, Strober S. Comparison of chimeric acid and non-chimeric tolerance using posttransplant total lymphoid irradiation: cytokine expression and chronic rejection. Transplantation. 1999;68:1036-44 pubmed
flow cytometry; rat	Hayamizu K, Huie P, Sibley R, Strober S. Monocyte-derived dendritic cell precursors facilitate tolerance to heart allografts after total lymphoid irradiation. Transplantation. 1998;66:1285-91 pubmed
flow cytometry; rat	Hayamizu K, Zeng D, Huie P, Garcia Ojeda M, Bloch D, Fong L, et al. Donor blood monocytes but not T or B cells facilitate long-term allograft survival after total lymphoid irradiation. Transplantation. 1998;66:585-93 pubmed
immunohistochemistry - frozen section; rat	Fathallah Shaykh H, Gao W, Cho M, Herrera M. Priming in the brain, an immunologically privileged organ, elicits anti-tumor immunity. Int J Cancer. 1998;75:266-76 pubmed
flow cytometry; rat immunoprecipitation; rat	Tamatani T, Kotani M, Miyasaka M. Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies. Eur J Immunol. 1991;21:627-33 pubmed
	Kitase Y, Chin E, Ramachandra S, Burkhardt C, Madurai N, Lenz C, et al. Sustained peripheral immune hyper-reactivity (SPIHR): an enduring biomarker of altered inflammatory responses in adult rats after perinatal brain injury. J Neuroinflammation. 2021;18:242 pubmed publisher
	Stevens A, Liu L, Bertovich D, Janjic J, Pollock J. Differential Expression of Neuroinflammatory mRNAs in the Rat Sciatic Nerve Following Chronic Constriction Injury and Pain-Relieving Nanoemulsion NSAID Delivery to Infiltrating Macrophages. Int J Mol Sci. 2019;20: pubmed publisher
	Bucan V, Fliess M, Schnabel R, Peck C, Vaslaitis D, Fülbier A, et al. In vitro enhancement and functional characterization of neurite outgrowth by undifferentiated adipose-derived stem cells. Int J Mol Med. 2019;43:593-602 pubmed publisher
	Wu Y, Shen L, Wang R, Tang J, Ding S, Wang S, et al. Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords. J Neurosci Res. 2018;96:1265-1276 pubmed publisher
	Deosarkar S, PRABHAKARPANDIAN B, Wang B, Sheffield J, Krynska B, Kiani M. A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip. PLoS ONE. 2015;10:e0142725 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD11b/c Monoclonal Antibody (OX-42)

Catalog # :

MA1-81606

Quantity :

100 ug

Price :

US 292.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Rat

Applications :

Flow Cytometry: 1:50-1:100, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:50-1:100, Immunoprecipitation: Assay-dependent

Species :

Rat

Clone :

OX-42

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.

Immunogen :

Rat peritoneal macrophages.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:50-1:100, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:50-1:100, Immunoprecipitation: Assay-dependent

Aliases :

AI449405; antigen CD11b (p170); CD11 antigen-like family member B; CD11 antigen-like family member C; CD11b; CD11B (p170); CD11b/CD18; CD11bc; CD11C; CD11C (p150) alpha polypeptide; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component 3 receptor 4 subunit; complement component receptor 3 alpha-a; complement receptor 4; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; Cr4; F730045J24Rik; integrin alpha M; integrin alpha X; integrin alpha-M; Integrin alpha-X; integrin aX; integrin subunit alpha M; integrin subunit alpha X; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); integrin, alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAM; ITGAX; Leu M5; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor MO1; leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; Ly-40; MAC1; MAC-1; Mac-1 alpha; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; myeloid membrane antigen, alpha subunit; N418; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; OX42; OX-42; p150 95 integrin alpha chain; RGD1561123; SLEB6

more info or order :

Invitrogen product webpage