product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CRALBP Monoclonal Antibody (B2)
catalog :
MA1-813
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B2
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; fig 3b
Qi X, Pay S, Yan Y, Thomas J, Lewin A, Chang L, et al. Systemic Injection of RPE65-Programmed Bone Marrow-Derived Cells Prevents Progression of Chronic Retinal Degeneration. Mol Ther. 2017;25:917-927 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3b
Choudhary P, Booth H, Gutteridge A, Surmacz B, Louca I, Steer J, et al. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage. Stem Cells Transl Med. 2017;6:490-501 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 1d
Choudhary P, Gutteridge A, Impey E, Storer R, Owen R, Whiting P, et al. Targeting the cAMP and Transforming Growth Factor-? Pathway Increases Proliferation to Promote Re-Epithelialization of Human Stem Cell-Derived Retinal Pigment Epithelium. Stem Cells Transl Med. 2016;5:925-37 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 4
  • immunohistochemistry; human; 1:400; fig s1
Wu W, Zeng Y, Li Z, Li Q, Xu H, Yin Z. Features specific to retinal pigment epithelium cells derived from three-dimensional human embryonic stem cell cultures - a new donor for cell therapy. Oncotarget. 2016;7:22819-33 pubmed publisher
  • western blot; human; fig 2
Wen B, Li S, Li H, Chen Y, Ma X, Wang J, et al. Microphthalmia-associated transcription factor regulates the visual cycle genes Rlbp1 and Rdh5 in the retinal pigment epithelium. Sci Rep. 2016;6:21208 pubmed publisher
  • immunohistochemistry; mouse; fig 6.a,b
Sun Y, Ju M, Lin Z, Fredrick T, Evans L, Tian K, et al. SOCS3 in retinal neurons and glial cells suppresses VEGF signaling to prevent pathological neovascular growth. Sci Signal. 2015;8:ra94 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
Choi V, Bigelow C, McGee T, Gujar A, Li H, Hanks S, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022 pubmed publisher
  • immunocytochemistry; human; 1:200
Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, et al. A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells. PLoS ONE. 2015;10:e0130379 pubmed publisher
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:5000
Muñiz A, Greene W, Plamper M, Choi J, Johnson A, Tsin A, et al. Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle. Invest Ophthalmol Vis Sci. 2014;55:198-209 pubmed publisher
  • immunohistochemistry; human
Powner M, Gillies M, Zhu M, Vevis K, Hunyor A, Fruttiger M. Loss of Müller's cells and photoreceptors in macular telangiectasia type 2. Ophthalmology. 2013;120:2344-52 pubmed publisher
  • immunocytochemistry; rat; 1:100
Ramirez M, Hernández Montoya J, Sánchez Serrano S, Ordaz B, Ferraro S, Quintero H, et al. GABA-mediated induction of early neuronal markers expression in postnatal rat progenitor cells in culture. Neuroscience. 2012;224:210-22 pubmed publisher
  • flow cytometry; human; 1:1000
Kuroda T, Yasuda S, Kusakawa S, Hirata N, Kanda Y, Suzuki K, et al. Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells. PLoS ONE. 2012;7:e37342 pubmed publisher
  • western blot; human; 1:2000
Ahmado A, Carr A, Vugler A, Semo M, Gias C, Lawrence J, et al. Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. Invest Ophthalmol Vis Sci. 2011;52:7148-59 pubmed publisher
  • immunohistochemistry; rat
Zaniolo K, Sapieha P, Shao Z, Stahl A, Zhu T, Tremblay S, et al. Ghrelin modulates physiologic and pathologic retinal angiogenesis through GHSR-1a. Invest Ophthalmol Vis Sci. 2011;52:5376-86 pubmed publisher
  • immunocytochemistry; rat
Ali T, Al Gayyar M, Matragoon S, Pillai B, Abdelsaid M, Nussbaum J, et al. Diabetes-induced peroxynitrite impairs the balance of pro-nerve growth factor and nerve growth factor, and causes neurovascular injury. Diabetologia. 2011;54:657-68 pubmed publisher
  • immunohistochemistry; human; fig 7
Powner M, Gillies M, Tretiach M, Scott A, Guymer R, Hageman G, et al. Perifoveal müller cell depletion in a case of macular telangiectasia type 2. Ophthalmology. 2010;117:2407-16 pubmed publisher
  • immunocytochemistry; mouse
Yanni S, McCollum G, Penn J. Genetic deletion of COX-2 diminishes VEGF production in mouse retinal Müller cells. Exp Eye Res. 2010;91:34-41 pubmed publisher
  • immunohistochemistry; rat; 1:1,000
Joly S, Pernet V, Chemtob S, Di Polo A, Lachapelle P. Neuroprotection in the juvenile rat model of light-induced retinopathy: evidence suggesting a role for FGF-2 and CNTF. Invest Ophthalmol Vis Sci. 2007;48:2311-20 pubmed
Hall J, Daniszewski M, Cheung S, Shobhana K, Kumar H, Liang H, et al. A semi-automated pipeline for quantifying drusen-like deposits in human induced pluripotent stem cell-derived retinal pigment epithelium cells. SLAS Technol. 2024;29:100106 pubmed publisher
Gallo R, Qureshi F, Strong T, Lang S, Pino K, Dvoriantchikova G, et al. Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines. Transl Vis Sci Technol. 2022;11:4 pubmed publisher
Shang P, Stepicheva N, Liu H, Chowdhury O, Franks J, Sun M, et al. A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for In Vitro Studies in AMD. Int J Mol Sci. 2021;22: pubmed publisher
Korecki A, Cueva Vargas J, Fornes O, Agostinone J, Farkas R, Hickmott J, et al. Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, corneal, endothelial, Müller glial, and PAX6 cells. Gene Ther. 2021;28:351-372 pubmed publisher
Bucher F, Aguilar E, Marra K, Rapp J, Arnold J, Diaz Aguilar S, et al. CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10. Invest Ophthalmol Vis Sci. 2020;61:20 pubmed publisher
Delaunay K, Khamsy L, Kowalczuk L, Moulin A, Nicolas M, Zografos L, et al. Glial cells of the human fovea. Mol Vis. 2020;26:235-245 pubmed
Powner M, Woods S, Zhu M, Gillies M, Bernstein P, Hageman G, et al. FUNDUS-WIDE SUBRETINAL AND PIGMENT EPITHELIAL ABNORMALITIES IN MACULAR TELANGIECTASIA TYPE 2. Retina. 2018;38 Suppl 1:S105-S113 pubmed publisher
Ramsden C, Nommiste B, R Lane A, Carr A, Powner M, J K Smart M, et al. Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs. Sci Rep. 2017;7:51 pubmed publisher
Vessey K, Waugh M, Jobling A, Phipps J, Ho T, Trogrlic L, et al. Assessment of retinal function and morphology in aging Ccl2 knockout mice. Invest Ophthalmol Vis Sci. 2015;56:1238-52 pubmed publisher
Kurumada S, Onishi A, Imai H, Ishii K, Kobayashi T, Sato S. Stage-specific association of apolipoprotein A-I and E in developing mouse retina. Invest Ophthalmol Vis Sci. 2007;48:1815-23 pubmed
Nawrot M, West K, Huang J, Possin D, Bretscher A, Crabb J, et al. Cellular retinaldehyde-binding protein interacts with ERM-binding phosphoprotein 50 in retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2004;45:393-401 pubmed
Budhu A, Noy N. Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest. Mol Cell Biol. 2002;22:2632-41 pubmed
Hind M, Corcoran J, Maden M. Temporal/spatial expression of retinoid binding proteins and RAR isoforms in the postnatal lung. Am J Physiol Lung Cell Mol Physiol. 2002;282:L468-76 pubmed
Bunt Milam A, Saari J. Immunocytochemical localization of two retinoid-binding proteins in vertebrate retina. J Cell Biol. 1983;97:703-12 pubmed
Crabb J, Gaur V, Garwin G, Marx S, Chapline C, Johnson C, et al. Topological and epitope mapping of the cellular retinaldehyde-binding protein from retina. J Biol Chem. 1991;266:16674-83 pubmed
product information
Product Type :
Antibody
Product Name :
CRALBP Monoclonal Antibody (B2)
Catalog # :
MA1-813
Quantity :
100 ug
Price :
US 471.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Bovine, Human, Mouse, Non-human primate, Rat
Applications :
Flow Cytometry: Assay-dependent, Immunohistochemistry (Frozen): 1 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 0.02 ug/mL
Species :
Bovine, Human, Mouse, Non-human primate, Rat
Clone :
B2
Isotype :
IgG1
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
Cellular retinaldehyde-binding protein (CRALBP) plays an important role in the regeneration of 11-cis-retinal for use in rod visual pigments such as opsin and rhodopsin. Once 11-cis-retinal is photoisomerized in the rod outer segment, it is converted to all-trans-retinal and further modified into all-trans-retinol. All-trans-retinol then diffuses into the retinal pigment epithelium (RPE) to be converted back to 11-cis-retinol and further oxidized into 11-cis-retinal (both by CRALBP). Genetic mutations involving CRALBP's lack of function have been linked to visual disease such as bothnia dystrophy, retinitis punctata albescens, retina pigmentosa, and Newfoundland rod-cone dystrophy. The presence of CRALBP serves as a marker for RPE and Muller glial cells of the retina.
Immunogen :
Human recombinant CRALBP.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-dependent, Immunohistochemistry (Frozen): 1 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 0.02 ug/mL
Aliases :
3110056M11Rik; cellular retinaldehyde-binding protein; cellular retinaldehyde-binding protein-1; CRALBP; retinaldehyde binding protein 1; retinaldehyde-binding protein 1; retinaldehyde-binding protein precursor; Rlbp1
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA