Granzyme B Monoclonal Antibody (GB11) | Invitrogen MA1-80734 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Granzyme B Monoclonal Antibody (GB11)

catalog :

MA1-80734

quantity :

100 ug

price :

US 532.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GB11

reactivity :

African green monkey, human, mouse, rhesus macaque

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, other

more info or order :

Invitrogen product webpage

citations: 79

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...	Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
flow cytometry; mouse; 1:200; loading ...	Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
immunocytochemistry; human; 10 ug/ml; loading ...; fig 6v1	Lafouresse F, Jugele R, Müller S, Doineau M, Duplan Eche V, Espinosa E, et al. Stochastic asymmetric repartition of lytic machinery in dividing CD8+ T cells generates heterogeneous killing behavior. elife. 2021;10: pubmed publisher
flow cytometry; human; loading ...; fig 4a	Liu G, Yu Y, Feng F, Zhu P, Zhang H, Zhang D, et al. Human CD8+CD28- T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo. BMC Immunol. 2020;21:23 pubmed publisher
other; human; 1:25; loading ...; fig 2f	Ramaglia V, Sheikh Mohamed S, Legg K, Park C, Rojas O, Zandee S, et al. Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry. elife. 2019;8: pubmed publisher
flow cytometry; human; loading ...; fig 2f	Provine N, Binder B, FitzPatrick M, Schuch A, Garner L, Williamson K, et al. Unique and Common Features of Innate-Like Human Vδ2+ γδT Cells and Mucosal-Associated Invariant T Cells. Front Immunol. 2018;9:756 pubmed publisher
flow cytometry; human; loading ...; fig 3b	Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed publisher
flow cytometry; human; loading ...; fig 3a	Gorvel L, Korenfeld D, Tung T, Klechevsky E. Dendritic Cell-Derived IL-32?: A Novel Inhibitory Cytokine of NK Cell Function. J Immunol. 2017;199:1290-1300 pubmed publisher
flow cytometry; human; loading ...; fig 3d	Tong A, Hashem H, Eid S, Allen F, Kingsley D, Huang A. Adoptive natural killer cell therapy is effective in reducing pulmonary metastasis of Ewing sarcoma. Oncoimmunology. 2017;6:e1303586 pubmed publisher
flow cytometry; human; loading ...; fig 3a	Cheuk S, Schlums H, Gallais Sérézal I, Martini E, Chiang S, Marquardt N, et al. CD49a Expression Defines Tissue-Resident CD8+ T Cells Poised for Cytotoxic Function in Human Skin. Immunity. 2017;46:287-300 pubmed publisher
flow cytometry; mouse; fig 6m	Knudson K, Pritzl C, Saxena V, Altman A, Daniels M, Teixeiro E. NFÎºB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality. Proc Natl Acad Sci U S A. 2017;114:E1659-E1667 pubmed publisher
flow cytometry; human; loading ...; fig 3c	Jeffery H, Jeffery L, Lutz P, Corrigan M, Webb G, Hirschfield G, et al. Low-dose interleukin-2 promotes STAT-5 phosphorylation, Treg survival and CTLA-4-dependent function in autoimmune liver diseases. Clin Exp Immunol. 2017;188:394-411 pubmed publisher
flow cytometry; human; loading ...	Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
flow cytometry; mouse; fig 1	Urban S, Berg L, Welsh R. Type 1 interferon licenses naÃ¯ve CD8 T cells to mediate anti-viral cytotoxicity. Virology. 2016;493:52-9 pubmed publisher
flow cytometry; mouse	Luo C, Liao W, Dadi S, Toure A, Li M. Graded Foxo1 activity in Treg cells differentiates tumour immunity from spontaneous autoimmunity. Nature. 2016;529:532-6 pubmed publisher
flow cytometry; mouse; 1:50; fig 5	Lucas T, Richner J, Diamond M. The Interferon-Stimulated Gene Ifi27l2a Restricts West Nile Virus Infection and Pathogenesis in a Cell-Type- and Region-Specific Manner. J Virol. 2015;90:2600-15 pubmed publisher
flow cytometry; mouse; loading ...; fig 2f	Littwitz Salomon E, Akhmetzyanova I, Vallet C, Francois S, Dittmer U, Gibbert K. Activated regulatory T cells suppress effector NK cell responses by an IL-2-mediated mechanism during an acute retroviral infection. Retrovirology. 2015;12:66 pubmed publisher
flow cytometry; mouse; loading ...; fig 10a	Herz J, Johnson K, McGavern D. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. J Exp Med. 2015;212:1153-69 pubmed publisher
flow cytometry; human; loading ...; fig 1	Bhela S, Kempsell C, Manohar M, Dominguez Villar M, Griffin R, Bhatt P, et al. Nonapoptotic and extracellular activity of granzyme B mediates resistance to regulatory T cell (Treg) suppression by HLA-DR-CD25hiCD127lo Tregs in multiple sclerosis and in response to IL-6. J Immunol. 2015;194:2180-9 pubmed publisher
flow cytometry; mouse	Wang X, Sumida H, Cyster J. GPR18 is required for a normal CD8Î±Î± intestinal intraepithelial lymphocyte compartment. J Exp Med. 2014;211:2351-9 pubmed publisher
flow cytometry; human; fig 5	Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Karbach J, et al. Immunomodulatory action of SGI-110, a hypomethylating agent, in acute myeloid leukemia cells and xenografts. Leuk Res. 2014;38:1332-41 pubmed publisher
flow cytometry; mouse; fig 7	Urban S, Welsh R. Out-of-sequence signal 3 as a mechanism for virus-induced immune suppression of CD8 T cell responses. PLoS Pathog. 2014;10:e1004357 pubmed publisher
flow cytometry; mouse; fig 2	Boding L, Hansen A, Meroni G, Johansen B, Braunstein T, Bonefeld C, et al. Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells. Eur J Immunol. 2014;44:3109-18 pubmed publisher
flow cytometry; mouse	Smith N, Wissink E, Wang J, Pinello J, Davenport M, Grimson A, et al. Rapid proliferation and differentiation impairs the development of memory CD8+ T cells in early life. J Immunol. 2014;193:177-84 pubmed publisher
flow cytometry; human	Bacchetta R, Lucarelli B, Sartirana C, Gregori S, Lupo Stanghellini M, Miqueu P, et al. Immunological Outcome in Haploidentical-HSC Transplanted Patients Treated with IL-10-Anergized Donor T Cells. Front Immunol. 2014;5:16 pubmed publisher
flow cytometry; human	Poonia B, Pauza C. Levels of CD56+TIM-3- effector CD8 T cells distinguish HIV natural virus suppressors from patients receiving antiretroviral therapy. PLoS ONE. 2014;9:e88884 pubmed publisher
flow cytometry; mouse	Joedicke J, Dietze K, Zelinskyy G, Dittmer U. The phenotype and activation status of regulatory T cells during Friend retrovirus infection. Virol Sin. 2014;29:48-60 pubmed publisher
flow cytometry; mouse	Kim E, Gasper D, Lee S, Plisch E, Svaren J, Suresh M. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J Immunol. 2014;192:985-95 pubmed publisher
immunocytochemistry; human; fig 1 western blot; human; fig 1	Tuli A, Thiery J, James A, Michelet X, Sharma M, Garg S, et al. Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity. Mol Biol Cell. 2013;24:3721-35 pubmed publisher
flow cytometry; mouse; fig 2	Tejera M, Kim E, Sullivan J, Plisch E, Suresh M. FoxO1 controls effector-to-memory transition and maintenance of functional CD8 T cell memory. J Immunol. 2013;191:187-99 pubmed publisher
flow cytometry; mouse; fig 4	Manzke N, Akhmetzyanova I, Hasenkrug K, Trilling M, Zelinskyy G, Dittmer U. CD4+ T cells develop antiretroviral cytotoxic activity in the absence of regulatory T cells and CD8+ T cells. J Virol. 2013;87:6306-13 pubmed publisher
flow cytometry; mouse; fig 2	Schmitt D, O Dee D, Brown M, Horzempa J, Russo B, Morel P, et al. Role of NK cells in host defense against pulmonary type A Francisella tularensis infection. Microbes Infect. 2013;15:201-11 pubmed publisher
flow cytometry; mouse; fig 2	Deguine J, Breart B, Lemaitre F, Bousso P. Cutting edge: tumor-targeting antibodies enhance NKG2D-mediated NK cell cytotoxicity by stabilizing NK cell-tumor cell interactions. J Immunol. 2012;189:5493-7 pubmed publisher
flow cytometry; mouse; fig 3	Bhadra R, Khan I. IL-7 and IL-15 do not synergize during CD8 T cell recall response against an obligate intracellular parasite. Microbes Infect. 2012;14:1160-8 pubmed publisher
flow cytometry; mouse	Zhang N, Bevan M. TGF-? signaling to T cells inhibits autoimmunity during lymphopenia-driven proliferation. Nat Immunol. 2012;13:667-73 pubmed publisher
flow cytometry; human; fig 5	Ribeiro Dos Santos P, Turnbull E, Monteiro M, Legrand A, Conrod K, Baalwa J, et al. Chronic HIV infection affects the expression of the 2 transcription factors required for CD8 T-cell differentiation into cytolytic effectors. Blood. 2012;119:4928-38 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 4a	Hutnick N, Myles D, Hirao L, Scott V, Ferraro B, Khan A, et al. An optimized SIV DNA vaccine can serve as a boost for Ad5 and provide partial protection from a high-dose SIVmac251 challenge. Vaccine. 2012;30:3202-8 pubmed publisher
flow cytometry; African green monkey; fig 6	CLAY C, Donart N, Fomukong N, Knight J, Lei W, Price L, et al. Primary severe acute respiratory syndrome coronavirus infection limits replication but not lung inflammation upon homologous rechallenge. J Virol. 2012;86:4234-44 pubmed publisher
flow cytometry; human	Randall K, Chan S, Ma C, Fung I, Mei Y, Yabas M, et al. DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice. J Exp Med. 2011;208:2305-20 pubmed publisher
flow cytometry; rhesus macaque; fig 2	Vargas Inchaustegui D, Demberg T, Robert Guroff M. A CD8?(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011;134:326-40 pubmed publisher
flow cytometry; mouse; fig 3	Brincks E, Gurung P, Langlois R, Hemann E, Legge K, Griffith T. The magnitude of the T cell response to a clinically significant dose of influenza virus is regulated by TRAIL. J Immunol. 2011;187:4581-8 pubmed publisher
flow cytometry; mouse; fig 4	Zelinskyy G, Myers L, Dietze K, Gibbert K, Roggendorf M, Liu J, et al. Virus-specific CD8+ T cells upregulate programmed death-1 expression during acute friend retrovirus infection but are highly cytotoxic and control virus replication. J Immunol. 2011;187:3730-7 pubmed publisher
flow cytometry; human; fig 3	Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure H, et al. Early induction of polyfunctional simian immunodeficiency virus (SIV)-specific T lymphocytes and rapid disappearance of SIV from lymph nodes of sooty mangabeys during primary infection. J Immunol. 2011;186:5151-61 pubmed publisher
flow cytometry; mouse; fig 2	Marshall H, Prince A, Berg L, Welsh R. IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions. J Immunol. 2010;185:1419-28 pubmed publisher
flow cytometry; mouse; fig 6	Tran Thang N, Derouazi M, Philippin G, Arcidiaco S, Di Berardino Besson W, Masson F, et al. Immune infiltration of spontaneous mouse astrocytomas is dominated by immunosuppressive cells from early stages of tumor development. Cancer Res. 2010;70:4829-39 pubmed publisher
immunocytochemistry; human	Thiery J, Keefe D, Saffarian S, Martinvalet D, Walch M, Boucrot E, et al. Perforin activates clathrin- and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis. Blood. 2010;115:1582-93 pubmed publisher
flow cytometry; mouse; fig 1	Brown D, Kamperschroer C, Dilzer A, Roberts D, Swain S. IL-2 and antigen dose differentially regulate perforin- and FasL-mediated cytolytic activity in antigen specific CD4+ T cells. Cell Immunol. 2009;257:69-79 pubmed publisher
flow cytometry; mouse; fig 5b	Hamada H, Garcia Hernandez M, Reome J, Misra S, Strutt T, McKinstry K, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81 pubmed publisher
flow cytometry; human	Huntington N, Legrand N, Alves N, Jaron B, Weijer K, Plet A, et al. IL-15 trans-presentation promotes human NK cell development and differentiation in vivo. J Exp Med. 2009;206:25-34 pubmed publisher
flow cytometry; human; fig 5	Brincks E, Katewa A, Kucaba T, Griffith T, Legge K. CD8 T cells utilize TRAIL to control influenza virus infection. J Immunol. 2008;181:4918-25 pubmed
flow cytometry; mouse; fig 3B flow cytometry; human; fig 5C	Masson F, Calzascia T, Di Berardino Besson W, De Tribolet N, Dietrich P, Walker P. Brain microenvironment promotes the final functional maturation of tumor-specific effector CD8+ T cells. J Immunol. 2007;179:845-53 pubmed
flow cytometry; mouse; fig 7	Love V, Grabie N, Duramad P, Stavrakis G, Sharpe A, Lichtman A. CTLA-4 ablation and interleukin-12 driven differentiation synergistically augment cardiac pathogenicity of cytotoxic T lymphocytes. Circ Res. 2007;101:248-57 pubmed
flow cytometry; mouse; fig 2	Coquet J, Kyparissoudis K, Pellicci D, Besra G, Berzins S, Smyth M, et al. IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 2007;178:2827-34 pubmed
flow cytometry; mouse	Obar J, Fuse S, Leung E, Bellfy S, Usherwood E. Gammaherpesvirus persistence alters key CD8 T-cell memory characteristics and enhances antiviral protection. J Virol. 2006;80:8303-15 pubmed
flow cytometry; mouse; fig 6	Yang T, Millar J, Groves T, Grinshtein N, Parsons R, Takenaka S, et al. The CD8+ T cell population elicited by recombinant adenovirus displays a novel partially exhausted phenotype associated with prolonged antigen presentation that nonetheless provides long-term immunity. J Immunol. 2006;176:200-10 pubmed
flow cytometry; mouse; fig 3	Matsuda J, Zhang Q, Ndonye R, Richardson S, Howell A, Gapin L. T-bet concomitantly controls migration, survival, and effector functions during the development of Valpha14i NKT cells. Blood. 2006;107:2797-805 pubmed
flow cytometry; mouse; fig 2	Klebanoff C, Gattinoni L, Torabi Parizi P, Kerstann K, Cardones A, Finkelstein S, et al. Central memory self/tumor-reactive CD8+ T cells confer superior antitumor immunity compared with effector memory T cells. Proc Natl Acad Sci U S A. 2005;102:9571-6 pubmed
	Kim H, Kim H, Nam M, Park J, Lee M, Chung S, et al. Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model. Cancer Cell Int. 2023;23:219 pubmed publisher
	Chen Z, Ruan W, Guo C, Chen K, Li L, Tian J, et al. Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas. Cell Prolif. 2023;56:e13400 pubmed publisher
	Lander V, Belle J, Kingston N, Herndon J, Hogg G, Liu X, et al. Stromal Reprogramming by FAK Inhibition Overcomes Radiation Resistance to Allow for Immune Priming and Response to Checkpoint Blockade. Cancer Discov. 2022;12:2774-2799 pubmed publisher
	Lee R, Li J, Li J, Wu C, Jiang S, Hsu W, et al. Synthetic Essentiality of Tryptophan 2,3-Dioxygenase 2 in APC-Mutated Colorectal Cancer. Cancer Discov. 2022;12:1702-1717 pubmed publisher
	Rahman S, Billingsley J, Sharma A, Styles T, Govindaraj S, Shanmugasundaram U, et al. Lymph node CXCR5+ NK cells associate with control of chronic SHIV infection. JCI Insight. 2022;7: pubmed publisher
	Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
	Carré T, Thiery J, Janji B, Terry S, Gros G, Meurice G, et al. Selection of tumor‑resistant variants following sustained natural killer cell‑mediated immune stress. Oncol Rep. 2021;45:582-594 pubmed publisher
	Thomé R, Boehm A, Ishikawa L, Casella G, Munhoz J, Ciric B, et al. Comprehensive Analysis of the Immune and Stromal Compartments of the CNS in EAE Mice Reveal Pathways by Which Chloroquine Suppresses Neuroinflammation. Brain Sci. 2020;10: pubmed publisher
	Schorer M, Rakebrandt N, Lambert K, Hunziker A, Pallmer K, Oxenius A, et al. TIGIT limits immune pathology during viral infections. Nat Commun. 2020;11:1288 pubmed publisher
	Zhang E, Ma Z, Li Q, Yan H, Liu J, Wu W, et al. TLR2 Stimulation Increases Cellular Metabolism in CD8+ T Cells and Thereby Enhances CD8+ T Cell Activation, Function, and Antiviral Activity. J Immunol. 2019;203:2872-2886 pubmed publisher
	Kuehlwein J, Borsche M, Korir P, Risch F, Mueller A, Hübner M, et al. Protection of Batf3-deficient mice from experimental cerebral malaria correlates with impaired cytotoxic T-cell responses and immune regulation. Immunology. 2020;159:193-204 pubmed publisher
	Sant S, Jenkins M, Dash P, Watson K, Wang Z, Pizzolla A, et al. Human γδ T-cell receptor repertoire is shaped by influenza viruses, age and tissue compartmentalisation. Clin Transl Immunology. 2019;8:e1079 pubmed publisher
	Taouk G, Hussein O, Zekak M, Abouelghar A, Al Sarraj Y, Abdelalim E, et al. CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse. Sci Rep. 2019;9:8756 pubmed publisher
	Bajor D, Mick R, Riese M, Huang A, Sullivan B, Richman L, et al. Long-term outcomes of a phase I study of agonist CD40 antibody and CTLA-4 blockade in patients with metastatic melanoma. Oncoimmunology. 2018;7:e1468956 pubmed publisher
	Lim E, Cugliandolo F, Rosner D, Gyori D, Roychoudhuri R, Okkenhaug K. Phosphoinositide 3-kinase δ inhibition promotes antitumor responses but antagonizes checkpoint inhibitors. JCI Insight. 2018;3: pubmed publisher
	Zaiatz Bittencourt V, Finlay D, Gardiner C. Canonical TGF-β Signaling Pathway Represses Human NK Cell Metabolism. J Immunol. 2018;200:3934-3941 pubmed publisher
	Pyle C, Uwadiae F, Swieboda D, Harker J. Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology. PLoS Pathog. 2017;13:e1006640 pubmed publisher
	Komlósi Z, Kovacs N, Van De Veen W, Kirsch A, Fahrner H, Wawrzyniak M, et al. Human CD40 ligand-expressing type 3 innate lymphoid cells induce IL-10-producing immature transitional regulatory B cells. J Allergy Clin Immunol. 2018;142:178-194.e11 pubmed publisher
	Miosge L, Sontani Y, Chuah A, Horikawa K, Russell T, Mei Y, et al. Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion. Proc Natl Acad Sci U S A. 2017;114:E5216-E5225 pubmed publisher
	Marshall N, Vong A, Devarajan P, Brauner M, Kuang Y, Nayar R, et al. NKG2C/E Marks the Unique Cytotoxic CD4 T Cell Subset, ThCTL, Generated by Influenza Infection. J Immunol. 2017;198:1142-1155 pubmed publisher
	Smithey M, Li G, Venturi V, Davenport M, Nikolich Zugich J. Lifelong persistent viral infection alters the naive T cell pool, impairing CD8 T cell immunity in late life. J Immunol. 2012;189:5356-66 pubmed publisher
	Lepisto A, Moser A, Zeh H, Lee K, Bartlett D, McKolanis J, et al. A phase I/II study of a MUC1 peptide pulsed autologous dendritic cell vaccine as adjuvant therapy in patients with resected pancreatic and biliary tumors. Cancer Ther. 2008;6:955-964 pubmed

product information

Product Type :

Antibody

Product Name :

Granzyme B Monoclonal Antibody (GB11)

Catalog # :

MA1-80734

Quantity :

100 ug

Price :

US 532.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Non-human primate, Rhesus Monkey

Applications :

ELISA: 2-5 ug/mL, Flow Cytometry: 1:10-1:100, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Human, Non-human primate, Rhesus Monkey

Clone :

GB11

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

Immunogen :

Purified human Granzyme B.

Format :

Liquid

Applications w/Dilutions :

ELISA: 2-5 ug/mL, Flow Cytometry: 1:10-1:100, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E

more info or order :

Invitrogen product webpage