CD9 Monoclonal Antibody (MM2/57) | Invitrogen MA1-80307 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD9 Monoclonal Antibody (MM2/57)

catalog :

MA1-80307

quantity :

100 ug

price :

US 474.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MM2/57

reactivity :

human, mouse, dogs, bovine, pigs , cat, domestic rabbit, domestic horse, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 1c	Koba T, Takeda Y, Narumi R, Shiromizu T, Nojima Y, Ito M, et al. Proteomics of serum extracellular vesicles identifies a novel COPD biomarker, fibulin-3 from elastic fibres. ERJ Open Res. 2021;7: pubmed publisher
flow cytometry; human; fig s1	Zhou P, Erfani S, Liu Z, Jia C, Chen Y, Xu B, et al. CD151-Î±3Î²1 integrin complexes are prognostic markers of glioblastoma and cooperate with EGFR to drive tumor cell motility and invasion. Oncotarget. 2015;6:29675-93 pubmed publisher
western blot; mouse; fig 1	Jin Y, Tachibana I, Takeda Y, He P, Kang S, Suzuki M, et al. Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages. PLoS ONE. 2013;8:e73706 pubmed publisher
flow cytometry; human; fig 6 western blot; human; fig 6	Kohmo S, Kijima T, Otani Y, Mori M, Minami T, Takahashi R, et al. Cell surface tetraspanin CD9 mediates chemoresistance in small cell lung cancer. Cancer Res. 2010;70:8025-35 pubmed publisher
western blot; human; fig 2	Castro Sanchez L, Soto Guzman A, Navarro Tito N, Martinez Orozco R, Salazar E. Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. Eur J Cell Biol. 2010;89:843-52 pubmed publisher
immunocytochemistry; human; fig 2 western blot; human; fig 5	Lafleur M, Xu D, Hemler M. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009;20:2030-40 pubmed publisher
western blot; human; fig 1	Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97 pubmed publisher
immunocytochemistry; human western blot; human; fig 2	Yang X, Richardson A, Torres Arzayus M, Zhou P, Sharma C, Kazarov A, et al. CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization. Cancer Res. 2008;68:3204-13 pubmed publisher
flow cytometry; human; fig 2A immunoprecipitation; human; fig 2B western blot; human; fig 2B	Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, et al. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006;66:9557-65 pubmed
	Juul N, Willacy O, Mamand D, Andaloussi S, Eisfeldt J, Chamorro C, et al. Insights into cellular behavior and micromolecular communication in urothelial micrografts. Sci Rep. 2023;13:13589 pubmed publisher
	Bui S, Dancourt J, Lavieu G. Virus-Free Method to Control and Enhance Extracellular Vesicle Cargo Loading and Delivery. ACS Appl Bio Mater. 2023;6:1081-1091 pubmed publisher
	Bernea E, Suica V, Uyy E, Cerveanu Hogas A, Boteanu R, Ivan L, et al. Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus. Molecules. 2022;27: pubmed publisher
	P xe9 rez Macedonio C, Flores Alfaro E, Alarc xf3 n Romero L, Vences Vel xe1 zquez A, Castro Alarc xf3 n N, Mart xed nez Mart xed nez E, et al. CD14 and CD26 from serum exosomes are associated with type 2 diabetes, exosomal Cystatin C and CD14 are associated with metabolic syndrome and atherogenic index of plasma. Peerj. 2022;10:e13656 pubmed publisher
	Tonner H, Hunn S, Auler N, Schmelter C, Beutgen V, von Pein H, et al. A Monoclonal Anti-HMGB1 Antibody Attenuates Neurodegeneration in an Experimental Animal Model of Glaucoma. Int J Mol Sci. 2022;23: pubmed publisher
	Alem F, Olanrewaju A, Omole S, Hobbs H, Ahsan N, Matulis G, et al. Exosomes originating from infection with the cytoplasmic single-stranded RNA virus Rift Valley fever virus (RVFV) protect recipient cells by inducing RIG-I mediated IFN-B response that leads to activation of autophagy. Cell Biosci. 2021;11:220 pubmed publisher
	Karttunen J, Stewart S, Kalmar L, Grant A, Karet Frankl F, Williams T. Size-Exclusion Chromatography Separation Reveals That Vesicular and Non-Vesicular Small RNA Profiles Differ in Cell Free Urine. Int J Mol Sci. 2021;22: pubmed publisher
	Lee C, Edman M, Laurie G, Hamm Alvarez S, MacKay J. Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium. Int J Mol Sci. 2020;21: pubmed publisher
	Es Haghi M, Godakumara K, Häling A, Lättekivi F, Lavrits A, Viil J, et al. Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk. Cell Commun Signal. 2019;17:146 pubmed publisher
	Jin Y, Takeda Y, Kondo Y, Tripathi L, Kang S, Takeshita H, et al. Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging. Sci Rep. 2018;8:5145 pubmed publisher
	Takeda Y, Tachibana I, Miyado K, Kobayashi M, Miyazaki T, Funakoshi T, et al. Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes. J Cell Biol. 2003;161:945-56 pubmed

product information

Product Type :

Antibody

Product Name :

CD9 Monoclonal Antibody (MM2/57)

Catalog # :

MA1-80307

Quantity :

100 ug

Price :

US 474.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Canine, Equine, Feline, Human, Mink, Porcine, Rabbit, Rhesus Monkey

Applications :

Flow Cytometry: 1:100-1:200, Immunohistochemistry (Frozen): 1:500-1:1,000, Western Blot: Assay-dependent

Species :

Bovine, Canine, Equine, Feline, Human, Mink, Porcine, Rabbit, Rhesus Monkey

Clone :

MM2/57

Isotype :

IgG2b

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

Immunogen :

Human platelet membrane.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100-1:200, Immunohistochemistry (Frozen): 1:500-1:1,000, Western Blot: Assay-dependent

Aliases :

5H9; 5H9 antigen; antigen CD9; BA2; BA-2/p24 antigen; BTCC-1; CD9; CD9 antigen; CD9 antigen (p24); CD9 molecule; cell growth-inhibiting gene 2 protein; DRAP-27; GIG2; Leukocyte antigen MIC3; MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin29; Tetraspanin-29; transmembrane protein CD9; TSPAN29; Tspan-29

more info or order :

Invitrogen product webpage