CD45 Monoclonal Antibody (YW62.3) | Invitrogen MA1-80090 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45 Monoclonal Antibody (YW62.3)

catalog :

MA1-80090

quantity :

100 ug

price :

US 245.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

YW62.3

reactivity :

mouse

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:10,000	Bachstetter A, Webster S, Goulding D, Morton J, Watterson D, Van Eldik L. Attenuation of traumatic brain injury-induced cognitive impairment in mice by targeting increased cytokine levels with a small molecule experimental therapeutic. J Neuroinflammation. 2015;12:69 pubmed publisher
immunohistochemistry; mouse; 1:10,000	Bachstetter A, Rowe R, Kaneko M, Goulding D, Lifshitz J, Van Eldik L. The p38? MAPK regulates microglial responsiveness to diffuse traumatic brain injury. J Neurosci. 2013;33:6143-53 pubmed publisher
flow cytometry; mouse	Grisaru D, Pick M, Perry C, Sklan E, Almog R, Goldberg I, et al. Hydrolytic and nonenzymatic functions of acetylcholinesterase comodulate hemopoietic stress responses. J Immunol. 2006;176:27-35 pubmed
flow cytometry; mouse; fig 5	Gupta R, Karpatkin S, Basch R. Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase. Blood. 2006;107:1837-46 pubmed
flow cytometry; mouse; fig 1	Chen B, Cui X, Sempowski G, Domen J, Chao N. Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation. Blood. 2004;103:4344-52 pubmed
flow cytometry; mouse	Chen B, Cui X, Sempowski G, Gooding M, Liu C, Haynes B, et al. A comparison of murine T-cell-depleted adult bone marrow and full-term fetal blood cells in hematopoietic engraftment and immune reconstitution. Blood. 2002;99:364-71 pubmed
	Pyka Fo x15b ciak G, Litwin J, Lis G. Osteopontin expression and the effect of anti-VLA-4 mAb treatment in experimental autoimmune encephalomyelitis, a mouse model of multiple sclerosis. Folia Neuropathol. 2023;61:129-137 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD45 Monoclonal Antibody (YW62.3)

Catalog # :

MA1-80090

Quantity :

100 ug

Price :

US 245.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 1:50-1:200, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent

Species :

Mouse

Clone :

YW62.3

Isotype :

IgG2b

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Immunogen :

Murine spleen cells.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:50-1:200, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent

Aliases :

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

more info or order :

Invitrogen product webpage