p53 Monoclonal Antibody (BD53-12) | Invitrogen MA1-7629 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p53 Monoclonal Antibody (BD53-12)

catalog :

MA1-7629

quantity :

100 ug

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BD53-12

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat	Kucuk A, Yucel M, Erkasap N, Tosun M, Koken T, Ozkurt M, et al. The effects of PDE5 inhibitory drugs on renal ischemia/reperfusion injury in rats. Mol Biol Rep. 2012;39:9775-82 pubmed publisher
immunohistochemistry; rat; 3 ug/ml	El Meghawry El Kenawy A, Osman H, Daghestani M. The effect of vitamin C administration on monosodium glutamate induced liver injury. An experimental study. Exp Toxicol Pathol. 2013;65:513-21 pubmed publisher
immunohistochemistry; rat; 1:100	Fouad A, Jresat I. Hepatoprotective effect of coenzyme Q10 in rats with acetaminophen toxicity. Environ Toxicol Pharmacol. 2012;33:158-67 pubmed publisher
western blot; rat; 1:400	Wang Q, Xu Y, Xie M, Yu Z, Qin Y, Wang W, et al. X-irradiation reduces the proliferation of astrocytes by cell cycle arrest. Neurosci Lett. 2011;498:78-83 pubmed publisher
immunohistochemistry; rat	Kucuk A, Kabadere S, Tosun M, Koken T, Kinaci M, Isikli B, et al. Protective effects of doxycycline in ischemia/reperfusion injury on kidney. J Physiol Biochem. 2009;65:183-91 pubmed
immunohistochemistry; mouse; 1:50	Greenow K, Clarke A, Jones R. Chk1 deficiency in the mouse small intestine results in p53-independent crypt death and subsequent intestinal compensation. Oncogene. 2009;28:1443-53 pubmed publisher
immunohistochemistry; rat; 1:50	Pedrycz A, Czerny K. Immunohistochemical study of proteins linked to apoptosis in rat fetal kidney cells following prepregnancy adriamycin administration in the mother. Acta Histochem. 2008;110:519-23 pubmed publisher
immunohistochemistry; rat	Arikan Y, Tosun M, Saykol V, Kalkan S, Erdem S. p53 Expression and apoptosis in liver and spleen during CO2 pneumoperitoneum. Langenbecks Arch Surg. 2008;393:877-82 pubmed
	Kim H, Kang B, Park S, Park H, Kim C, Lee H, et al. Forkhead box protein D2 suppresses colorectal cancer by reprogramming enhancer interactions. Nucleic Acids Res. 2023;51:6143-6155 pubmed publisher

product information

Product Type :

Antibody

Product Name :

p53 Monoclonal Antibody (BD53-12)

Catalog # :

MA1-7629

Quantity :

100 ug

Price :

US 524.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ChIP Assay: 2 uL/10^6 cells, ELISA: 1:500, Flow Cytometry: Assay-dependent, Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent

Species :

Human, Mouse, Rat

Clone :

BD53-12

Isotype :

IgG2a

Storage :

4 C short term, -80 C long term

Description :

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

Immunogen :

p53.

Format :

Liquid

Applications w/Dilutions :

Aliases :

Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3

more info or order :

Invitrogen product webpage