SNTB2 Monoclonal Antibody (1351) | Invitrogen MA1-745 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SNTB2 Monoclonal Antibody (1351)

catalog :

MA1-745

quantity :

100 ug

price :

US 491.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1351

reactivity :

human, mouse, rat, dogs, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; fig 2	Sicca F, Ambrosini E, Marchese M, Sforna L, Servettini I, Valvo G, et al. Gain-of-function defects of astrocytic Kir4.1 channels in children with autism spectrum disorders and epilepsy. Sci Rep. 2016;6:34325 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig 3	Gonzalez J, Crassous P, Schneider J, Beuve A, Fraidenraich D. Neuronal nitric oxide synthase localizes to utrophin expressing intercalated discs and stabilizes their structural integrity. Neuromuscul Disord. 2015;25:964-76 pubmed publisher
western blot; human; 1:200	Ambrosini E, Sicca F, Brignone M, D Adamo M, Napolitano C, Servettini I, et al. Genetically induced dysfunctions of Kir2.1 channels: implications for short QT3 syndrome and autism-epilepsy phenotype. Hum Mol Genet. 2014;23:4875-86 pubmed publisher
western blot; human	Makokha G, Takahashi M, Higuchi M, Saito S, Tanaka Y, Fujii M. Human T-cell leukemia virus type 1 Tax protein interacts with and mislocalizes the PDZ domain protein MAGI-1. Cancer Sci. 2013;104:313-20 pubmed publisher
western blot; mouse; fig 7	Lien C, Mohanta S, Frontczak Baniewicz M, Swinny J, Zablocka B, Gorecki D. Absence of glial ?-dystrobrevin causes abnormalities of the blood-brain barrier and progressive brain edema. J Biol Chem. 2012;287:41374-85 pubmed publisher
western blot; human; 1:100	Beck A, Vitale J, Zhao Q, Schneider J, Chang C, Altaf A, et al. Differential requirement for utrophin in the induced pluripotent stem cell correction of muscle versus fat in muscular dystrophy mice. PLoS ONE. 2011;6:e20065 pubmed publisher
immunohistochemistry; rat; 1:100 immunocytochemistry; dogs; 1:100 western blot; dogs; 1:500	Tham D, Moukhles H. Regulation of Kir4.1 and AQP4 expression and stability at the basolateral domain of epithelial MDCK cells by the extracellular matrix. Am J Physiol Renal Physiol. 2011;301:F396-409 pubmed publisher
immunocytochemistry; mouse; 1:80	Duarri A, López de Heredia M, Capdevila Nortes X, Ridder M, Montolio M, López Hernández T, et al. Knockdown of MLC1 in primary astrocytes causes cell vacuolation: a MLC disease cell model. Neurobiol Dis. 2011;43:228-38 pubmed publisher
western blot; rat; 1:1000	Noel G, STEVENSON S, Moukhles H. A high throughput screen identifies chemical modulators of the laminin-induced clustering of dystroglycan and aquaporin-4 in primary astrocytes. PLoS ONE. 2011;6:e17559 pubmed publisher
immunohistochemistry; mouse	Bragg A, Das S, Froehner S. Dystrophin-associated protein scaffolding in brain requires alpha-dystrobrevin. Neuroreport. 2010;21:695-9 pubmed publisher
western blot; human	Yamashita K, Suzuki A, Satoh Y, Ide M, Amano Y, Masuda Hirata M, et al. The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b. Biochem Biophys Res Commun. 2010;391:812-7 pubmed publisher
western blot; rat; 1:2000	Lanciotti A, Brignone M, Camerini S, Serafini B, Macchia G, Raggi C, et al. MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation. Neurobiol Dis. 2010;37:581-95 pubmed publisher
immunohistochemistry; rat; 1:100	Lui L, Levinson J, Noel G, Handrigan G, Richman J, El Husseini A, et al. Synaptic localization of neuroligin 2 in the rodent retina: comparative study with the dystroglycan-containing complex. J Neurosci Res. 2010;88:837-49 pubmed publisher
immunoprecipitation; human immunocytochemistry; human; 5-10 ug/ml western blot; human	Costantini J, Cheung S, Hou S, Li H, Kung S, Johnston J, et al. TAPP2 links phosphoinositide 3-kinase signaling to B-cell adhesion through interaction with the cytoskeletal protein utrophin: expression of a novel cell adhesion-promoting complex in B-cell leukemia. Blood. 2009;114:4703-12 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Stillwell E, Vitale J, Zhao Q, Beck A, Schneider J, Khadim F, et al. Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice. PLoS ONE. 2009;4:e4759 pubmed publisher
western blot; mouse	Gavillet B, Rougier J, Domenighetti A, Behar R, Boixel C, Ruchat P, et al. Cardiac sodium channel Nav1.5 is regulated by a multiprotein complex composed of syntrophins and dystrophin. Circ Res. 2006;99:407-14 pubmed
immunoprecipitation; human western blot; human	Okuhira K, Fitzgerald M, Sarracino D, Manning J, Bell S, Goss J, et al. Purification of ATP-binding cassette transporter A1 and associated binding proteins reveals the importance of beta1-syntrophin in cholesterol efflux. J Biol Chem. 2005;280:39653-64 pubmed
	Campelj D, Timpani C, Cree T, Petersen A, Hayes A, Goodman C, et al. Metronomic 5-Fluorouracil Delivery Primes Skeletal Muscle for Myopathy but Does Not Cause Cachexia. Pharmaceuticals (Basel). 2021;14: pubmed publisher
	Ye R, Onodera T, Blanchard P, Kusminski C, Esser V, Brekken R, et al. β1 Syntrophin Supports Autophagy Initiation and Protects against Cerulein-Induced Acute Pancreatitis. Am J Pathol. 2019;189:813-825 pubmed publisher
	Shy D, Gillet L, Ogrodnik J, Albesa M, Verkerk A, Wolswinkel R, et al. PDZ domain-binding motif regulates cardiomyocyte compartment-specific NaV1.5 channel expression and function. Circulation. 2014;130:147-60 pubmed publisher
	Fratini F, Macchia G, Torreri P, Matteucci A, Salzano A, Crescenzi M, et al. Phosphorylation on threonine 11 of ?-dystrobrevin alters its interaction with kinesin heavy chain. FEBS J. 2012;279:4131-44 pubmed publisher
	Brignone M, Lanciotti A, Macioce P, Macchia G, Gaetani M, Aloisi F, et al. The beta1 subunit of the Na,K-ATPase pump interacts with megalencephalic leucoencephalopathy with subcortical cysts protein 1 (MLC1) in brain astrocytes: new insights into MLC pathogenesis. Hum Mol Genet. 2011;20:90-103 pubmed publisher
	De Arcangelis V, Serra F, Cogoni C, Vivarelli E, Monaco L, Naro F. ?1-syntrophin modulation by miR-222 in mdx mice. PLoS ONE. 2010;5: pubmed publisher
	Chen Z, Hague C, Hall R, Minneman K. Syntrophins regulate alpha1D-adrenergic receptors through a PDZ domain-mediated interaction. J Biol Chem. 2006;281:12414-20 pubmed
	Ort T, Voronov S, Guo J, Zawalich K, Froehner S, Zawalich W, et al. Dephosphorylation of beta2-syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion. EMBO J. 2001;20:4013-23 pubmed
	Ort T, Maksimova E, Dirkx R, Kachinsky A, Berghs S, Froehner S, et al. The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta-cells. Eur J Cell Biol. 2000;79:621-30 pubmed
	Adams M, Kramarcy N, Krall S, Rossi S, Rotundo R, Sealock R, et al. Absence of alpha-syntrophin leads to structurally aberrant neuromuscular synapses deficient in utrophin. J Cell Biol. 2000;150:1385-98 pubmed
	Dai Z, Peng H. A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation. J Cell Biol. 1998;141:1613-24 pubmed
	Gee S, Madhavan R, Levinson S, Caldwell J, Sealock R, Froehner S. Interaction of muscle and brain sodium channels with multiple members of the syntrophin family of dystrophin-associated proteins. J Neurosci. 1998;18:128-37 pubmed
	Peters M, Adams M, Froehner S. Differential association of syntrophin pairs with the dystrophin complex. J Cell Biol. 1997;138:81-93 pubmed
	Froehner S, Murnane A, Tobler M, Peng H, Sealock R. A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle. J Cell Biol. 1987;104:1633-46 pubmed

product information

Product Type :

Antibody

Product Name :

SNTB2 Monoclonal Antibody (1351)

Catalog # :

MA1-745

Quantity :

100 ug

Price :

US 491.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Amphibian, Canine, Chicken, Human, Mouse, Rat

Applications :

Flow Cytometry: 0.5 ug / 10^6 cells, Immunocytochemistry: 1-2 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 0.1-1 ug/mL

Species :

Amphibian, Canine, Chicken, Human, Mouse, Rat

Clone :

1351

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Syntrophins are structurally related proteins located at the neuromuscular synapse and play an important role in dystrophin associated protein (DAP) complex formation. To date there have been three distinct syntrophin isoforms discovered: alpha 1, beta 1, and beta 2. These proteins each contain a PDZ domain, a syntrophin-unique domain (SU), and three pleckstrin homology domains (PH1a,1b and 2). The PDZ domain has been shown to be responsible for binding syntrophin to nNOS and the DAP complex. This association results in localization of the nicotinic acetylcholine receptor to the cytoskeleton via the (S/T)XV C terminus.

Immunogen :

Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 ug / 10^6 cells, Immunocytochemistry: 1-2 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 0.1-1 ug/mL

Aliases :

59 kDa dystrophin-associated protein A1 basic component 2; beta-2-syntrophin; D16S2531E; dystrophin-associated protein A1, 59kD, basic component 2; EST25263; Snt2; SNT2B2; SNT3; SNTB2; SNTL; SYN; syntrophin beta 2; syntrophin, basic 2; syntrophin, beta 2; syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2); syntrophin-3; Syntrophin-like

more info or order :

Invitrogen product webpage