GCAP1 Monoclonal Antibody (G2) | Invitrogen MA1-724 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GCAP1 Monoclonal Antibody (G2)

catalog :

MA1-724

quantity :

100 ug

price :

US 414.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, bovine

application :

western blot

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human	Codega P, Della Santina L, Gargini C, Bedolla D, Subkhankulova T, Livesey F, et al. Prolonged illumination up-regulates arrestin and two guanylate cyclase activating proteins: a novel mechanism for light adaptation. J Physiol. 2009;587:2457-72 pubmed publisher
	Sokal I, Otto Bruc A, Surgucheva I, Verlinde C, Wang C, Baehr W, et al. Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration. J Biol Chem. 1999;274:19829-37 pubmed
	Otto Bruc A, Fariss R, Van Hooser J, Palczewski K. Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxin. Proc Natl Acad Sci U S A. 1998;95:15014-9 pubmed
	Otto Bruc A, Fariss R, Haeseleer F, Huang J, Buczyłko J, Surgucheva I, et al. Localization of guanylate cyclase-activating protein 2 in mammalian retinas. Proc Natl Acad Sci U S A. 1997;94:4727-32 pubmed
	Otto Bruc A, Buczylko J, Surgucheva I, Subbaraya I, Rudnicka Nawrot M, Crabb J, et al. Functional reconstitution of photoreceptor guanylate cyclase with native and mutant forms of guanylate cyclase-activating protein 1. Biochemistry. 1997;36:4295-302 pubmed
	Gorczyca W, Polans A, Surgucheva I, Subbaraya I, Baehr W, Palczewski K. Guanylyl cyclase activating protein. A calcium-sensitive regulator of phototransduction. J Biol Chem. 1995;270:22029-36 pubmed
	Palczewski K, Subbaraya I, Gorczyca W, Helekar B, Ruiz C, Ohguro H, et al. Molecular cloning and characterization of retinal photoreceptor guanylyl cyclase-activating protein. Neuron. 1994;13:395-404 pubmed

product information

Product Type :

Antibody

Product Name :

GCAP1 Monoclonal Antibody (G2)

Catalog # :

MA1-724

Quantity :

100 ug

Price :

US 414.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Human, Non-human primate

Applications :

Western Blot: 1 ug/mL

Species :

Bovine, Human, Non-human primate

Clone :

Isotype :

IgG2a

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

Immunogen :

Bacterial expressed full length GCAP-1.

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1 ug/mL

Aliases :

C6orf131; COD3; cone dystrophy 3; CORD14; GC-A; GCAP; GCAP 1; GCAP1; Guanylate cyclase activator 1A; guanylate cyclase activator 1a (retina); guanylate cyclase-activating protein, photoreceptor 1; guanylin 1, retina; guanylyl cyclase-activating protein; guanylyl cyclase-activating protein 1; GUCA; GUCA1; GUCA1A; mGCAP1

more info or order :

Invitrogen product webpage