Rhodopsin Monoclonal Antibody (1D4) | Invitrogen MA1-722 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Rhodopsin Monoclonal Antibody (1D4)

catalog :

MA1-722

quantity :

100 uL

price :

US 471.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D4

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s2	Biswas P, Berry A, Zawaydeh Q, Bartsch D, Raghavendra P, Hejtmancik J, et al. A Mouse Model with Ablated Asparaginase and Isoaspartyl Peptidase 1 (Asrgl1) Develops Early Onset Retinal Degeneration (RD) Recapitulating the Human Phenotype. Genes (Basel). 2022;13: pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 1c	Ebke L, Sinha S, Pauer G, Hagstrom S. Photoreceptor Compartment-Specific TULP1 Interactomes. Int J Mol Sci. 2021;22: pubmed publisher
immunocytochemistry; mouse; ; loading ...; fig 2a	Guzmán Mendoza N, Homma K, Osada H, Toda E, Ban N, Nagai N, et al. Neuroprotective Effect of 4-Phenylbutyric Acid against Photo-Stress in the Retina. Antioxidants (Basel). 2021;10: pubmed publisher
western blot; human; loading ...; fig 4a	Jastrzebska B, Comar W, Kaliszewski M, Skinner K, Torcasio M, Esway A, et al. A G Protein-Coupled Receptor Dimerization Interface in Human Cone Opsins. Biochemistry. 2017;56:61-72 pubmed publisher
immunocytochemistry; human; loading ...; fig s5a	Tao L, Zhang J, Meraner P, Tovaglieri A, Wu X, Gerhard R, et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature. 2016;538:350-355 pubmed publisher
immunocytochemistry; human; 1:1000 western blot; human; 1:5000	Molday L, Molday R. 1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins. Methods Mol Biol. 2014;1177:1-15 pubmed publisher
immunocytochemistry; human; 1:1000	Bailes H, Zhuang L, Lucas R. Reproducible and sustained regulation of G?s signalling using a metazoan opsin as an optogenetic tool. PLoS ONE. 2012;7:e30774 pubmed publisher
western blot; human	Junge H, Yang S, Burton J, Paes K, Shu X, French D, et al. TSPAN12 regulates retinal vascular development by promoting Norrin- but not Wnt-induced FZD4/beta-catenin signaling. Cell. 2009;139:299-311 pubmed publisher
western blot; rat; 1:2,000	Tanito M, Kaidzu S, Anderson R. Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats. Invest Ophthalmol Vis Sci. 2007;48:1864-72 pubmed
	Tian S, Xiong X, Zeng J, Wang S, Tremblay B, Chen P, et al. Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants. Nat Commun. 2022;13:6786 pubmed publisher
	Bloemeke N, Meighen Berger K, Hitzenberger M, Bach N, Parr M, Coelho J, et al. Intramembrane client recognition potentiates the chaperone functions of calnexin. EMBO J. 2022;41:e110959 pubmed publisher
	Harte J, Wakerlin S, Lindsay A, McCarthy J, Coleman Vaughan C. Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2. Viruses. 2022;14: pubmed publisher
	Ozawa Y, Toda E, Homma K, Osada H, Nagai N, Tsubota K, et al. Effects of Epigenetic Modification of PGC-1α by a Chemical Chaperon on Mitochondria Biogenesis and Visual Function in Retinitis Pigmentosa. Cells. 2022;11: pubmed publisher
	Zhou Y, Tian W, Jiang X, Yang H, Jiang Z, Li X, et al. Deletion of Asrgl1 Leads to Photoreceptor Degeneration in Mice. Front Cell Dev Biol. 2021;9:783547 pubmed publisher
	Pan Z, Zhang Y, Luo J, Li D, Zhou Y, He L, et al. Functional analyses of epidemic Clostridioides difficile toxin B variants reveal their divergence in utilizing receptors and inducing pathology. PLoS Pathog. 2021;17:e1009197 pubmed publisher
	Li Y, Wang H, Tang X, Fang S, Ma D, Du C, et al. SARS-CoV-2 and Three Related Coronaviruses Utilize Multiple ACE2 Orthologs and Are Potently Blocked by an Improved ACE2-Ig. J Virol. 2020;94: pubmed publisher
	Tian S, Liu Y, Wu H, Liu H, Zeng J, Choi M, et al. Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL. Cell Host Microbe. 2020;27:782-792.e7 pubmed publisher
	Xu H, Qu C, Gan L, Sun K, Tan J, Liu X, et al. Deletion of the Impg2 gene causes the degeneration of rod and cone cells in mice. Hum Mol Genet. 2020;29:1624-1634 pubmed publisher
	Starr C, Nyankerh C, Qi X, Hu Y, Gorbatyuk O, Sonenberg N, et al. Role of Translational Attenuation in Inherited Retinal Degeneration. Invest Ophthalmol Vis Sci. 2019;60:4849-4857 pubmed publisher
	Tian S, Wu Q, Zhou B, Choi M, Ding B, Yang W, et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep. 2019;28:2517-2526.e5 pubmed publisher
	Kanan Y, Khan M, Lorenc V, Long D, Chadha R, Sciamanna J, et al. Metipranolol promotes structure and function of retinal photoreceptors in the rd10 mouse model of human retinitis pigmentosa. J Neurochem. 2019;148:307-318 pubmed publisher
	Zhang L, Sun Z, Zhao P, Huang L, Xu M, Yang Y, et al. Whole-exome sequencing revealed HKDC1 as a candidate gene associated with autosomal-recessive retinitis pigmentosa. Hum Mol Genet. 2018;27:4157-4168 pubmed publisher
	Kang Y, Kuybeda O, de Waal P, Mukherjee S, Van Eps N, Dutka P, et al. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature. 2018;558:553-558 pubmed publisher
	Mishra A, Das B, Nath M, Iyer S, Kesarwani A, Bhattacharjee J, et al. A novel immunodeficient NOD.SCID-rd1 mouse model of retinitis pigmentosa to investigate potential therapeutics and pathogenesis of retinal degeneration. Biol Open. 2017;6:449-462 pubmed publisher
	Chalat M, Moleschi K, Molday R. C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity. Mol Biol Cell. 2017;28:452-462 pubmed publisher
	Bakondi B, Lv W, Lu B, Jones M, Tsai Y, Kim K, et al. In Vivo CRISPR/Cas9 Gene Editing Corrects Retinal Dystrophy in the S334ter-3 Rat Model of Autosomal Dominant Retinitis Pigmentosa. Mol Ther. 2016;24:556-63 pubmed publisher
	Zulliger R, Conley S, Mwoyosvi M, Stuck M, Azadi S, Naash M. SNAREs Interact with Retinal Degeneration Slow and Rod Outer Segment Membrane Protein-1 during Conventional and Unconventional Outer Segment Targeting. PLoS ONE. 2015;10:e0138508 pubmed publisher
	Becirovic E, Nguyen O, Paparizos C, Butz E, Stern Schneider G, Wolfrum U, et al. Peripherin-2 couples rhodopsin to the CNG channel in outer segments of rod photoreceptors. Hum Mol Genet. 2014;23:5989-97 pubmed publisher
	Michalakis S, Shaltiel L, Sothilingam V, Koch S, Schludi V, Krause S, et al. Mosaic synaptopathy and functional defects in Cav1.4 heterozygous mice and human carriers of CSNB2. Hum Mol Genet. 2014;23:1538-50 pubmed publisher
	Bailes H, Lucas R. Human melanopsin forms a pigment maximally sensitive to blue light (?max ? 479 nm) supporting activation of G(q/11) and G(i/o) signalling cascades. Proc Biol Sci. 2013;280:20122987 pubmed publisher
	Chen C, Woodruff M, Chen F, Chen Y, Cilluffo M, Tranchina D, et al. Modulation of mouse rod response decay by rhodopsin kinase and recoverin. J Neurosci. 2012;32:15998-6006 pubmed publisher
	Kono M. Assays for inverse agonists in the visual system. Methods Enzymol. 2010;485:213-24 pubmed publisher
	Kono M, Crouch R. In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists. Methods Mol Biol. 2010;652:85-94 pubmed publisher
	Tanito M, Brush R, Elliott M, Wicker L, Henry K, Anderson R. High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration. J Lipid Res. 2009;50:807-19 pubmed publisher
	Sakurai K, Onishi A, Imai H, Chisaka O, Ueda Y, Usukura J, et al. Physiological properties of rod photoreceptor cells in green-sensitive cone pigment knock-in mice. J Gen Physiol. 2007;130:21-40 pubmed
	Yoshida Y, Murakami A, Iwai K, Tanaka K. A neural-specific F-box protein Fbs1 functions as a chaperone suppressing glycoprotein aggregation. J Biol Chem. 2007;282:7137-44 pubmed
	Li F, Cao W, Anderson R. Alleviation of constant-light-induced photoreceptor degeneration by adaptation of adult albino rat to bright cyclic light. Invest Ophthalmol Vis Sci. 2003;44:4968-75 pubmed
	Castagnet P, Mavlyutov T, Cai Y, Zhong F, Ferreira P. RPGRIP1s with distinct neuronal localization and biochemical properties associate selectively with RanBP2 in amacrine neurons. Hum Mol Genet. 2003;12:1847-63 pubmed
	Moritz O, Tam B, Papermaster D, Nakayama T. A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern. J Biol Chem. 2001;276:28242-51 pubmed
	Farzan M, Schnitzler C, Vasilieva N, Leung D, Kuhn J, Gerard C, et al. Sulfated tyrosines contribute to the formation of the C5a docking site of the human C5a anaphylatoxin receptor. J Exp Med. 2001;193:1059-66 pubmed
	Redmond T, Yu S, Lee E, Bok D, Hamasaki D, Chen N, et al. Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle. Nat Genet. 1998;20:344-51 pubmed
	Cideciyan A, Hood D, Huang Y, Banin E, Li Z, Stone E, et al. Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man. Proc Natl Acad Sci U S A. 1998;95:7103-8 pubmed
	DeCaluwé G, Degrip W. Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin. Biochem J. 1996;320 ( Pt 3):807-15 pubmed
	Molday R, Mackenzie D. Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes. Biochemistry. 1983;22:653-60 pubmed
	Hodges R, Heaton R, Parker J, Molday L, Molday R. Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin. J Biol Chem. 1988;263:11768-75 pubmed

product information

Product Type :

Antibody

Product Name :

Rhodopsin Monoclonal Antibody (1D4)

Catalog # :

MA1-722

Quantity :

100 uL

Price :

US 471.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000

Species :

Bovine, Human, Mouse, Rat

Clone :

1D4

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

Immunogen :

Bovine rhodopsin.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000

Aliases :

CSNBAD1; L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; Noerg1; OPN 2; Opn2; Ops; opsin 2; opsin 2, rod pigment; opsin-2; Red Opsin; retinal rod opsin pigment rh1.1; Rh; RH1; rh1.1; rho; rho protein; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); rhodopsin-like-1.1 rod opsin; Rod Opsin; RP 4; RP4; seven transmembrane light receptor; unknown opsin-like protein; wu:fi06d11; zfo2; zfrho

more info or order :

Invitrogen product webpage