GRK1 Monoclonal Antibody (D11) | Invitrogen MA1-721 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GRK1 Monoclonal Antibody (D11)

catalog :

MA1-721

quantity :

100 ug

price :

US 440.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D11

reactivity :

human, mouse, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse western blot; mouse	Osawa S, Jo R, Xiong Y, Reidel B, Tserentsoodol N, Arshavsky V, et al. Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors. J Biol Chem. 2011;286:20923-9 pubmed publisher
	Chrispell J, Xiong Y, Weiss E. Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP. J Biol Chem. 2022;298:102636 pubmed publisher
	Sudharsan R, Simone K, Anderson N, Aguirre G, Beltran W. Acute and Protracted Cell Death in Light-Induced Retinal Degeneration in the Canine Model of Rhodopsin Autosomal Dominant Retinitis Pigmentosa. Invest Ophthalmol Vis Sci. 2017;58:270-281 pubmed publisher
	Tsang S, Woodruff M, Chen C, Yamashita C, Cilluffo M, Rao A, et al. GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase. J Neurosci. 2006;26:4472-80 pubmed
	Young J, Vogt T, Gross K, Khani S. A short, highly active photoreceptor-specific enhancer/promoter region upstream of the human rhodopsin kinase gene. Invest Ophthalmol Vis Sci. 2003;44:4076-85 pubmed
	Zhu X, Brown B, Li A, Mears A, Swaroop A, Craft C. GRK1-dependent phosphorylation of S and M opsins and their binding to cone arrestin during cone phototransduction in the mouse retina. J Neurosci. 2003;23:6152-60 pubmed

product information

Product Type :

Antibody

Product Name :

GRK1 Monoclonal Antibody (D11)

Catalog # :

MA1-721

Quantity :

100 ug

Price :

US 440.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Human, Mouse

Applications :

Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Frozen): 1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Bovine, Human, Mouse

Clone :

D11

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.

Immunogen :

Full length human GRK1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Frozen): 1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

G protein-coupled receptor kinase 1; G protein-coupled receptpr kinase 1; GPRK1; G-protein coupled receptor kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1; GRK1a; rhodopsin kinase; rhodopsin kinase GRK1; Rhok; RK

more info or order :

Invitrogen product webpage