GRK1 Monoclonal Antibody (G8) | Invitrogen MA1-720 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GRK1 Monoclonal Antibody (G8)

catalog :

MA1-720

quantity :

100 ug

price :

US 451.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000	Reish N, Maltare A, McKeown A, Laszczyk A, Kraft T, Gross A, et al. The age-regulating protein klotho is vital to sustain retinal function. Invest Ophthalmol Vis Sci. 2013;54:6675-85 pubmed publisher
immunohistochemistry; mouse	Sun X, Haley J, Bulgakov O, Cai X, McGinnis J, Li T. Tubby is required for trafficking G protein-coupled receptors to neuronal cilia. Cilia. 2012;1:21 pubmed publisher
western blot; mouse; 1:5000	Chen C, Woodruff M, Chen F, Chen Y, Cilluffo M, Tranchina D, et al. Modulation of mouse rod response decay by rhodopsin kinase and recoverin. J Neurosci. 2012;32:15998-6006 pubmed publisher
western blot; mouse; 1:5,000	Chen C, Woodruff M, Chen F, Shim H, Cilluffo M, Fain G. Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay. J Physiol. 2010;588:3231-41 pubmed publisher
immunohistochemistry; mouse; 1:200 western blot; mouse; 1:5,000	Chen C, Woodruff M, Chen F, Chen D, Fain G. Background light produces a recoverin-dependent modulation of activated-rhodopsin lifetime in mouse rods. J Neurosci. 2010;30:1213-20 pubmed publisher
western blot; human	Doan T, Azevedo A, Hurley J, Rieke F. Arrestin competition influences the kinetics and variability of the single-photon responses of mammalian rod photoreceptors. J Neurosci. 2009;29:11867-79 pubmed publisher
western blot; mouse; 1:500	Wen X, Shen L, Brush R, Michaud N, Al Ubaidi M, Gurevich V, et al. Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors. Biophys J. 2009;96:939-50 pubmed publisher
immunohistochemistry; rat; 1:1000	Fawcett R, Osborne N. Flupirtine attenuates sodium nitroprusside-induced damage to retinal photoreceptors, in situ. Brain Res Bull. 2007;73:278-88 pubmed
western blot; bovine western blot; mouse	Krispel C, Chen D, Melling N, Chen Y, Martemyanov K, Quillinan N, et al. RGS expression rate-limits recovery of rod photoresponses. Neuron. 2006;51:409-16 pubmed
immunocytochemistry; mouse; 1:500	Zhao X, Liu J, Ahmad I. Differentiation of embryonic stem cells to retinal cells in vitro. Methods Mol Biol. 2006;330:401-16 pubmed
western blot; mouse	Kerov V, Chen D, Moussaif M, Chen Y, Chen C, Artemyev N. Transducin activation state controls its light-dependent translocation in rod photoreceptors. J Biol Chem. 2005;280:41069-76 pubmed
	Chrispell J, Xiong Y, Weiss E. Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP. J Biol Chem. 2022;298:102636 pubmed publisher
	Dilan T, Moye A, Salido E, Saravanan T, Kolandaivelu S, Goldberg A, et al. ARL13B, a Joubert Syndrome-Associated Protein, Is Critical for Retinogenesis and Elaboration of Mouse Photoreceptor Outer Segments. J Neurosci. 2019;39:1347-1364 pubmed publisher
	Gross O, Burns M. Control of rhodopsin's active lifetime by arrestin-1 expression in mammalian rods. J Neurosci. 2010;30:3450-7 pubmed publisher
	Beltran W, Allore H, Johnson E, Towle V, Tao W, Acland G, et al. CREB1/ATF1 activation in photoreceptor degeneration and protection. Invest Ophthalmol Vis Sci. 2009;50:5355-63 pubmed publisher
	Tanito M, Brush R, Elliott M, Wicker L, Henry K, Anderson R. High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration. J Lipid Res. 2009;50:807-19 pubmed publisher
	Osborne N, Wood J. The beta-adrenergic receptor antagonist metipranolol blunts zinc-induced photoreceptor and RPE apoptosis. Invest Ophthalmol Vis Sci. 2006;47:3178-86 pubmed
	Kasus Jacobi A, Ou J, Birch D, Locke K, Shelton J, Richardson J, et al. Functional characterization of mouse RDH11 as a retinol dehydrogenase involved in dark adaptation in vivo. J Biol Chem. 2005;280:20413-20 pubmed
	Norton A, Hosier S, Terew J, Li N, Dhingra A, Vardi N, et al. Evaluation of the 17-kDa prenyl-binding protein as a regulatory protein for phototransduction in retinal photoreceptors. J Biol Chem. 2005;280:1248-56 pubmed
	Ghosh S, Salvador Silva M, Coca Prados M. The bovine iris-ciliary epithelium expresses components of rod phototransduction. Neurosci Lett. 2004;370:7-12 pubmed
	Ramamurthy V, Niemi G, Reh T, Hurley J. Leber congenital amaurosis linked to AIPL1: a mouse model reveals destabilization of cGMP phosphodiesterase. Proc Natl Acad Sci U S A. 2004;101:13897-902 pubmed
	Cideciyan A, Jacobson S, Gupta N, Osawa S, Locke K, Weiss E, et al. Cone deactivation kinetics and GRK1/GRK7 expression in enhanced S cone syndrome caused by mutations in NR2E3. Invest Ophthalmol Vis Sci. 2003;44:1268-74 pubmed
	Zhao X, Yokoyama K, Whitten M, Huang J, Gelb M, Palczewski K. A novel form of rhodopsin kinase from chicken retina and pineal gland. FEBS Lett. 1999;454:115-21 pubmed
	Otto Bruc A, Fariss R, Van Hooser J, Palczewski K. Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxin. Proc Natl Acad Sci U S A. 1998;95:15014-9 pubmed

product information

Product Type :

Antibody

Product Name :

GRK1 Monoclonal Antibody (G8)

Catalog # :

MA1-720

Quantity :

100 ug

Price :

US 451.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Chicken, Human, Mouse, Rat

Applications :

Immunocytochemistry: 2-3 ug/mL, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 ug/mL

Species :

Bovine, Chicken, Human, Mouse, Rat

Clone :

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.

Immunogen :

Full length human GRK1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2-3 ug/mL, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 ug/mL

Aliases :

G protein-coupled receptor kinase 1; G protein-coupled receptpr kinase 1; GPRK1; G-protein coupled receptor kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1; GRK1a; rhodopsin kinase; rhodopsin kinase GRK1; Rhok; RK

more info or order :

Invitrogen product webpage