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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
VDR Monoclonal Antibody (9A7)
catalog :
MA1-710
quantity :
100 µg
price :
US 430.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
9A7
reactivity :
hamsters, human, mouse, rat, dogs, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 116
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; loading ...; fig 1a
Al Azhri J, Zhang Y, Bshara W, Zirpoli G, McCann S, Khoury T, et al. Tumor Expression of Vitamin D Receptor and Breast Cancer Histopathological Characteristics and Prognosis. Clin Cancer Res. 2017;23:97-103 pubmed publisher
  • western blot; human; fig 3
Verone Boyle A, Shoemaker S, Attwood K, Morrison C, Makowski A, Battaglia S, et al. Diet-derived 25-hydroxyvitamin D3 activates vitamin D receptor target gene expression and suppresses EGFR mutant non-small cell lung cancer growth in vitro and in vivo. Oncotarget. 2016;7:995-1013 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250; fig 2d
Mazzilli S, Hershberger P, Reid M, Bogner P, Atwood K, Trump D, et al. Vitamin D Repletion Reduces the Progression of Premalignant Squamous Lesions in the NTCU Lung Squamous Cell Carcinoma Mouse Model. Cancer Prev Res (Phila). 2015;8:895-904 pubmed publisher
  • western blot; mouse; fig 6.a,b
Iwahori S, Hakki M, Chou S, Kalejta R. Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97. J Biol Chem. 2015;290:19666-80 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 3
  • western blot; mouse
Bothwell K, Shaurova T, Merzianu M, Suresh A, Kuriakose M, Johnson C, et al. Impact of Short-term 1,25-Dihydroxyvitamin D3 on the Chemopreventive Efficacy of Erlotinib against Oral Cancer. Cancer Prev Res (Phila). 2015;8:765-76 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse; 1:500
Bach F, Rutten K, Hendriks K, Riemers F, Cornelissen P, de Bruin A, et al. The paracrine feedback loop between vitamin D? (1,25(OH)?D?) and PTHrP in prehypertrophic chondrocytes. J Cell Physiol. 2014;229:1999-2014 pubmed publisher
  • western blot; human; 1:200
Wahler J, So J, Kim Y, Liu F, Maehr H, Uskokovic M, et al. Inhibition of the transition of ductal carcinoma in situ to invasive ductal carcinoma by a Gemini vitamin D analog. Cancer Prev Res (Phila). 2014;7:617-26 pubmed publisher
  • immunohistochemistry; hamsters; 1:150
Ford J, Carnes K, Hess R. Ductuli efferentes of the male Golden Syrian hamster reproductive tract. Andrology. 2014;2:510-20 pubmed publisher
  • immunohistochemistry; human
Yuan F, Valiyaparambil J, Woods M, Tran H, Pant R, Adams J, et al. Vitamin D signaling regulates oral keratinocyte proliferation in vitro and in vivo. Int J Oncol. 2014;44:1625-33 pubmed publisher
  • immunocytochemistry; human
Campos L, Brentani H, Roela R, Katayama M, Lima L, Rolim C, et al. Differences in transcriptional effects of 1?,25 dihydroxyvitamin D3 on fibroblasts associated to breast carcinomas and from paired normal breast tissues. J Steroid Biochem Mol Biol. 2013;133:12-24 pubmed publisher
  • western blot; human
Jiang Y, Fleet J. Phorbol esters enhance 1?,25-dihydroxyvitamin D3-regulated 25-hydroxyvitamin D-24-hydroxylase (CYP24A1) gene expression through ERK-mediated phosphorylation of specific protein 3 (Sp3) in Caco-2 cells. Mol Cell Endocrinol. 2012;361:31-9 pubmed publisher
  • immunohistochemistry; human; 1:500
Suetani R, Ho K, Jindal S, Manavis J, Neilsen P, Pishas K, et al. A comparison of vitamin D activity in paired non-malignant and malignant human breast tissues. Mol Cell Endocrinol. 2012;362:202-10 pubmed publisher
  • immunohistochemistry; mouse
  • western blot; mouse
Wang Y, Zhu J, DeLuca H. Where is the vitamin D receptor?. Arch Biochem Biophys. 2012;523:123-33 pubmed publisher
  • immunohistochemistry; human
Parker Autry C, Burgio K, Richter H. Vitamin D status: a review with implications for the pelvic floor. Int Urogynecol J. 2012;23:1517-26 pubmed publisher
  • western blot; human
Zhang Q, Kanterewicz B, Buch S, Petkovich M, Parise R, Beumer J, et al. CYP24 inhibition preserves 1?,25-dihydroxyvitamin D(3) anti-proliferative signaling in lung cancer cells. Mol Cell Endocrinol. 2012;355:153-61 pubmed publisher
  • immunohistochemistry; chicken; 1:100
  • western blot; chicken; 1:1000
Wojtusik J, Johnson P. Vitamin D regulates anti-Mullerian hormone expression in granulosa cells of the hen. Biol Reprod. 2012;86:91 pubmed publisher
  • immunocytochemistry; human
Ching S, Kashinkunti S, Niehaus M, Zinser G. Mammary adipocytes bioactivate 25-hydroxyvitamin D? and signal via vitamin D? receptor, modulating mammary epithelial cell growth. J Cell Biochem. 2011;112:3393-405 pubmed publisher
  • immunohistochemistry; mouse; 1:60
  • western blot; mouse
Welsh J, Zinser L, Mianecki Morton L, Martin J, Waltz S, James H, et al. Age-related changes in the epithelial and stromal compartments of the mammary gland in normocalcemic mice lacking the vitamin D3 receptor. PLoS ONE. 2011;6:e16479 pubmed publisher
  • western blot; human
Alimirah F, Peng X, Murillo G, Mehta R. Functional significance of vitamin D receptor FokI polymorphism in human breast cancer cells. PLoS ONE. 2011;6:e16024 pubmed publisher
  • immunohistochemistry; human
Wang Y, DeLuca H. Is the vitamin d receptor found in muscle?. Endocrinology. 2011;152:354-63 pubmed publisher
  • immunocytochemistry; human
Hertting O, Holm A, Lüthje P, Brauner H, Dyrdak R, Jonasson A, et al. Vitamin D induction of the human antimicrobial Peptide cathelicidin in the urinary bladder. PLoS ONE. 2010;5:e15580 pubmed publisher
  • immunohistochemistry; human
Srinivasan M, Parwani A, Hershberger P, Lenzner D, Weissfeld J. Nuclear vitamin D receptor expression is associated with improved survival in non-small cell lung cancer. J Steroid Biochem Mol Biol. 2011;123:30-6 pubmed publisher
  • immunohistochemistry; dogs
Malone E, Rassnick K, Wakshlag J, Russell D, Al Sarraf R, Ruslander D, et al. Calcitriol (1,25-dihydroxycholecalciferol) enhances mast cell tumour chemotherapy and receptor tyrosine kinase inhibitor activity in vitro and has single-agent activity against spontaneously occurring canine mast cell tumours. Vet Comp Oncol. 2010;8:209-20 pubmed publisher
  • western blot; human
Peleg S, Nguyen C. The importance of nuclear import in protection of the vitamin D receptor from polyubiquitination and proteasome-mediated degradation. J Cell Biochem. 2010;110:926-34 pubmed publisher
  • western blot; human; 1:2000
Zhang Z, Kovalenko P, Cui M, Desmet M, Clinton S, Fleet J. Constitutive activation of the mitogen-activated protein kinase pathway impairs vitamin D signaling in human prostate epithelial cells. J Cell Physiol. 2010;224:433-42 pubmed publisher
  • immunohistochemistry; human
Russell D, Rassnick K, Erb H, Vaughan M, McDonough S. An immunohistochemical study of vitamin D receptor expression in canine cutaneous mast cell tumours. J Comp Pathol. 2010;143:223-6 pubmed publisher
  • immunoprecipitation; rat; 1:200
Zhao G, Simpson R. Interaction between vitamin D receptor with caveolin-3 and regulation by 1,25-dihydroxyvitamin D3 in adult rat cardiomyocytes. J Steroid Biochem Mol Biol. 2010;121:159-63 pubmed publisher
  • western blot; mouse
Lee H, So J, DeCastro A, Smolarek A, Paul S, Maehr H, et al. Gemini vitamin D analog suppresses ErbB2-positive mammary tumor growth via inhibition of ErbB2/AKT/ERK signaling. J Steroid Biochem Mol Biol. 2010;121:408-12 pubmed publisher
  • western blot; human; 1:4,000
Washington M, Weigel N. 1{alpha},25-Dihydroxyvitamin D3 inhibits growth of VCaP prostate cancer cells despite inducing the growth-promoting TMPRSS2:ERG gene fusion. Endocrinology. 2010;151:1409-17 pubmed publisher
  • western blot; human
Silvagno F, De Vivo E, Attanasio A, Gallo V, Mazzucco G, Pescarmona G. Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. PLoS ONE. 2010;5:e8670 pubmed publisher
  • immunoprecipitation; rat
Zhao G, Simpson R. Membrane localization, Caveolin-3 association and rapid actions of vitamin D receptor in cardiac myocytes. Steroids. 2010;75:555-9 pubmed publisher
  • western blot; human
Chow E, Sun H, Khan A, Groothuis G, Pang K. Effects of 1alpha,25-dihydroxyvitamin D3 on transporters and enzymes of the rat intestine and kidney in vivo. Biopharm Drug Dispos. 2010;31:91-108 pubmed publisher
  • EMSA; human
Dwivedi P, Gao X, Tan J, Evdokiou A, Ferrante A, Morris H, et al. A role for the phosphatidylinositol 3-kinase--protein kinase C zeta--Sp1 pathway in the 1,25-dihydroxyvitamin D3 induction of the 25-hydroxyvitamin D3 24-hydroxylase gene in human kidney cells. Cell Signal. 2010;22:543-52 pubmed publisher
  • western blot; mouse; 1:1500
Gonzalez Pardo V, Martin D, Gutkind J, Verstuyf A, Bouillon R, de Boland A, et al. 1 Alpha,25-dihydroxyvitamin D3 and its TX527 analog inhibit the growth of endothelial cells transformed by Kaposi sarcoma-associated herpes virus G protein-coupled receptor in vitro and in vivo. Endocrinology. 2010;151:23-31 pubmed publisher
  • western blot; rat; 1:2000
Chow E, Maeng H, Liu S, Khan A, Groothuis G, Pang K. 1alpha,25-Dihydroxyvitamin D(3) triggered vitamin D receptor and farnesoid X receptor-like effects in rat intestine and liver in vivo. Biopharm Drug Dispos. 2009;30:457-75 pubmed publisher
  • western blot; chicken
Ellis B, Gattoni Celli S, Mancia A, Kindy M. The vitamin D3 transcriptomic response in skin cells derived from the Atlantic bottlenose dolphin. Dev Comp Immunol. 2009;33:901-12 pubmed publisher
  • immunohistochemistry; human; 1:400
Pospechova K, Rozehnal V, Stejskalova L, Vrzal R, Pospisilova N, Jamborova G, et al. Expression and activity of vitamin D receptor in the human placenta and in choriocarcinoma BeWo and JEG-3 cell lines. Mol Cell Endocrinol. 2009;299:178-87 pubmed publisher
  • western blot; human
Kemmis C, Welsh J. Mammary epithelial cell transformation is associated with deregulation of the vitamin D pathway. J Cell Biochem. 2008;105:980-8 pubmed publisher
  • immunocytochemistry; human; 10 ug/ml
Du W, Cui Z, Tsui Z, Chen Q, Willingham M. In situ immunocytochemical detection of altered membrane composition induced by cell-cell contact in cultured mammalian cells. Microsc Res Tech. 2008;71:749-59 pubmed publisher
  • western blot; rat; 1:1000
Gonzalez Pardo V, Boland R, de Boland A. Vitamin D receptor levels and binding are reduced in aged rat intestinal subcellular fractions. Biogerontology. 2008;9:109-18 pubmed
  • western blot; human
Rapuri P, Gallagher J, Nawaz Z. Caffeine decreases vitamin D receptor protein expression and 1,25(OH)2D3 stimulated alkaline phosphatase activity in human osteoblast cells. J Steroid Biochem Mol Biol. 2007;103:368-71 pubmed
  • western blot; human; 1:200
Bosse Y, Maghni K, Hudson T. 1alpha,25-dihydroxy-vitamin D3 stimulation of bronchial smooth muscle cells induces autocrine, contractility, and remodeling processes. Physiol Genomics. 2007;29:161-8 pubmed
  • immunocytochemistry; mouse
Baroni E, Biffi M, Benigni F, Monno A, Carlucci D, Carmeliet G, et al. VDR-dependent regulation of mast cell maturation mediated by 1,25-dihydroxyvitamin D3. J Leukoc Biol. 2007;81:250-62 pubmed
  • immunohistochemistry; human; 1:100
Yang A, Hsu C, Chen J, Tseng L, Won G, Lee C. Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands. Virchows Arch. 2006;449:62-8 pubmed
  • immunohistochemistry; mouse
Benigni F, Baroni E, Zecevic M, Zvara P, Streng T, Hedlund P, et al. Oral treatment with a vitamin D3 analogue (BXL628) has anti-inflammatory effects in rodent model of interstitial cystitis. BJU Int. 2006;97:617-24 pubmed
  • EMSA; human
Sepulveda V, Weigel N, Falzon M. Prostate cancer cell type-specific involvement of the VDR and RXR in regulation of the human PTHrP gene via a negative VDRE. Steroids. 2006;71:102-15 pubmed
  • immunocytochemistry; human; 1:100
Diaw L, Roth M, Schwinn D, d Alelio M, Green L, Tangrea J. Characteristics of a human prostate stromal cell line related to its use in a stromal-epithelial coculture model for the study of cancer chemoprevention. In Vitro Cell Dev Biol Anim. 2005;41:142-8 pubmed
  • immunohistochemistry; chicken
Milliken E, Zhang X, Flask C, Duerk J, MacDonald P, Keri R. EB1089, a vitamin D receptor agonist, reduces proliferation and decreases tumor growth rate in a mouse model of hormone-induced mammary cancer. Cancer Lett. 2005;229:205-15 pubmed
  • EMSA; human
McGaffin K, Chrysogelos S. Identification and characterization of a response element in the EGFR promoter that mediates transcriptional repression by 1,25-dihydroxyvitamin D3 in breast cancer cells. J Mol Endocrinol. 2005;35:117-33 pubmed
  • western blot; mouse
Nakagawa K, Kawaura A, Kato S, Takeda E, Okano T. 1 alpha,25-Dihydroxyvitamin D(3) is a preventive factor in the metastasis of lung cancer. Carcinogenesis. 2005;26:429-40 pubmed
Lee D, Kim S, Koo Y, Chae Y, Wang J, Kim S, et al. Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases. Front Vet Sci. 2023;10:1265203 pubmed publisher
Zhang Q, Jia R, Chen M, Wang J, Huang F, Shi M, et al. Antagonizing EZH2 combined with vitamin D3 exerts a synergistic role in anti-fibrosis through bidirectional effects on hepatocytes and hepatic stellate cells. J Gastroenterol Hepatol. 2023;38:441-450 pubmed publisher
Zheng Z, Xie J, Ma L, Hao Z, Zhang W, Li L. Vitamin D Receptor Activation Targets ROS-Mediated Crosstalk Between Autophagy and Apoptosis in Hepatocytes in Cholestasic Mice. Cell Mol Gastroenterol Hepatol. 2023;15:887-901 pubmed publisher
Slominski A, Bro x17c yna A, Kim T, Elsayed M, Janjetovic Z, Qayyum S, et al. CYP11A1‑derived vitamin D hydroxyderivatives as candidates for therapy of basal and squamous cell carcinomas. Int J Oncol. 2022;61: pubmed publisher
Holah N. The Clinical Value of VDR and CTLA 4 in Evaluating the Prognosis of Invasive Duct Carcinoma of Egyptian Patients and their Benefit as a Target Therapy. Asian Pac J Cancer Prev. 2021;22:1183-1194 pubmed publisher
Hui Q, Zhao X, Lu P, Liu S, Nyachoti M, O K, et al. Molecular distribution and localization of extracellular calcium-sensing receptor (CaSR) and vitamin D receptor (VDR) at three different laying stages in laying hens (Gallus gallus domesticus). Poult Sci. 2021;100:101060 pubmed publisher
Grzesiak M, Burzawa G, Kurowska P, Blaszczyk K, Szlaga A, Blasiak A, et al. Altered vitamin D3 metabolism in the ovary and periovarian adipose tissue of rats with letrozole-induced PCOS. Histochem Cell Biol. 2021;155:101-116 pubmed publisher
Slominski A, Brozyna A, Zmijewski M, Janjetovic Z, Kim T, Slominski R, et al. The Role of Classical and Novel Forms of Vitamin D in the Pathogenesis and Progression of Nonmelanoma Skin Cancers. Adv Exp Med Biol. 2020;1268:257-283 pubmed publisher
Verma A, Vincent Chong V, Dejong H, Hershberger P, Seshadri M. Impact of dietary vitamin D on initiation and progression of oral cancer. J Steroid Biochem Mol Biol. 2020;199:105603 pubmed publisher
Gooding S, Olechnowicz S, Morris E, Armitage A, Arezes J, Frost J, et al. Transcriptomic profiling of the myeloma bone-lining niche reveals BMP signalling inhibition to improve bone disease. Nat Commun. 2019;10:4533 pubmed publisher
Zhu X, Wu S, Guo H. Active Vitamin D and Vitamin D Receptor Help Prevent High Glucose Induced Oxidative Stress of Renal Tubular Cells via AKT/UCP2 Signaling Pathway. Biomed Res Int. 2019;2019:9013904 pubmed publisher
Vincent Chong V, Dejong H, Attwood K, Hershberger P, Seshadri M. Preclinical Prevention Trial of Calcitriol: Impact of Stage of Intervention and Duration of Treatment on Oral Carcinogenesis. Neoplasia. 2019;21:376-388 pubmed publisher
Lin Y, Sun H, Chiu W, Su H, Chien Y, Chong L, et al. Calcitriol Inhibits HCV Infection via Blockade of Activation of PPAR and Interference with Endoplasmic Reticulum-Associated Degradation. Viruses. 2018;10: pubmed publisher
Battaglia S, Karasik E, Gillard B, Williams J, Winchester T, Moser M, et al. LSD1 dual function in mediating epigenetic corruption of the vitamin D signaling in prostate cancer. Clin Epigenetics. 2017;9:82 pubmed publisher
Janmaat V, van de Winkel A, Peppelenbosch M, Spaander M, Uitterlinden A, Pourfarzad F, et al. Vitamin D Receptor Polymorphisms Are Associated with Reduced Esophageal Vitamin D Receptor Expression and Reduced Esophageal Adenocarcinoma Risk. Mol Med. 2015;21:346-54 pubmed publisher
Blumberg J, Tzameli I, Astapova I, Lam F, Flier J, Hollenberg A. Complex role of the vitamin D receptor and its ligand in adipogenesis in 3T3-L1 cells. J Biol Chem. 2006;281:11205-13 pubmed
Miyauchi Y, Michigami T, Sakaguchi N, Sekimoto T, Yoneda Y, Pike J, et al. Importin 4 is responsible for ligand-independent nuclear translocation of vitamin D receptor. J Biol Chem. 2005;280:40901-8 pubmed
Mallya S, Gallagher J, Wild Y, Kifor O, Costa Guda J, Saucier K, et al. Abnormal parathyroid cell proliferation precedes biochemical abnormalities in a mouse model of primary hyperparathyroidism. Mol Endocrinol. 2005;19:2603-9 pubmed
Beer T, Myrthue A, Garzotto M, O Hara M, Chin R, Lowe B, et al. Randomized study of high-dose pulse calcitriol or placebo prior to radical prostatectomy. Cancer Epidemiol Biomarkers Prev. 2004;13:2225-32 pubmed
Capiati D, Rossi A, Picotto G, Benassati S, Boland R. Inhibition of serum-stimulated mitogen activated protein kinase by 1alpha,25(OH)2-vitamin D3 in MCF-7 breast cancer cells. J Cell Biochem. 2004;93:384-97 pubmed
Zinser G, Welsh J. Vitamin D receptor status alters mammary gland morphology and tumorigenesis in MMTV-neu mice. Carcinogenesis. 2004;25:2361-72 pubmed
Narayanan R, Sepulveda V, Falzon M, Weigel N. The functional consequences of cross-talk between the vitamin D receptor and ERK signaling pathways are cell-specific. J Biol Chem. 2004;279:47298-310 pubmed
Zinser G, Welsh J. Accelerated mammary gland development during pregnancy and delayed postlactational involution in vitamin D3 receptor null mice. Mol Endocrinol. 2004;18:2208-23 pubmed
Christensen G, Jepsen J, Fog C, Christensen I, Lykkesfeldt A. Sequential versus combined treatment of human breast cancer cells with antiestrogens and the vitamin D analogue EB1089 and evaluation of predictive markers for vitamin D treatment. Breast Cancer Res Treat. 2004;85:53-63 pubmed
Krishan A, Arya P, Ganjei Azar P, Shirley S, Escoffery C, Nadji M. Androgen and vitamin D receptor expression in archival human breast tumors. Cytometry B Clin Cytom. 2004;58:53-60 pubmed
Ismail A, Nguyen C, Ahene A, Fleet J, Uskokovic M, Peleg S. Effect of cellular environment on the selective activation of the vitamin D receptor by 1alpha,25-dihydroxyvitamin D3 and its analog 1alpha-fluoro-16-ene-20-epi-23-ene-26,27-bishomo-25-hydroxyvitamin D3 (Ro-26-9228). Mol Endocrinol. 2004;18:874-87 pubmed
Barbosa E, Nonogaki S, Katayama M, Folgueira M, Alves V, Brentani M. Vitamin D3 modulation of plasminogen activator inhibitor type-1 in human breast carcinomas under organ culture. Virchows Arch. 2004;444:175-82 pubmed
Zhang C, Dowd D, Staal A, Gu C, Lian J, Van Wijnen A, et al. Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing. J Biol Chem. 2003;278:35325-36 pubmed
Flanagan L, Packman K, Juba B, O Neill S, Tenniswood M, Welsh J. Efficacy of Vitamin D compounds to modulate estrogen receptor negative breast cancer growth and invasion. J Steroid Biochem Mol Biol. 2003;84:181-92 pubmed
Fleet J, Eksir F, Hance K, Wood R. Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines. Am J Physiol Gastrointest Liver Physiol. 2002;283:G618-25 pubmed
Erben R, Soegiarto D, Weber K, Zeitz U, Lieberherr M, Gniadecki R, et al. Deletion of deoxyribonucleic acid binding domain of the vitamin D receptor abrogates genomic and nongenomic functions of vitamin D. Mol Endocrinol. 2002;16:1524-37 pubmed
Barletta F, Freedman L, Christakos S. Enhancement of VDR-mediated transcription by phosphorylation: correlation with increased interaction between the VDR and DRIP205, a subunit of the VDR-interacting protein coactivator complex. Mol Endocrinol. 2002;16:301-14 pubmed
Sunn K, Cock T, Crofts L, Eisman J, Gardiner E. Novel N-terminal variant of human VDR. Mol Endocrinol. 2001;15:1599-609 pubmed
David J, Rincon M, Neff L, Horne W, Baron R. Carbonic anhydrase II is an AP-1 target gene in osteoclasts. J Cell Physiol. 2001;188:89-97 pubmed
Issa L, Leong G, Barry J, Sutherland R, Eisman J. Glucocorticoid receptor-interacting protein-1 and receptor-associated coactivator-3 differentially interact with the vitamin D receptor (VDR) and regulate VDR-retinoid X receptor transcriptional cross-talk. Endocrinology. 2001;142:1606-15 pubmed
Shang Y, Hu X, DiRenzo J, Lazar M, Brown M. Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription. Cell. 2000;103:843-52 pubmed
Liu Y, Nguyen C, Peleg S. Regulation of ligand-induced heterodimerization and coactivator interaction by the activation function-2 domain of the vitamin D receptor. Mol Endocrinol. 2000;14:1776-87 pubmed
Nemere I, Campbell K. Immunochemical studies on the putative plasmalemmal receptor for 1, 25-dihydroxyvitamin D(3). III. Vitamin D status. Steroids. 2000;65:451-7 pubmed
Nemere I, Ray R, McManus W. Immunochemical studies on the putative plasmalemmal receptor for 1, 25(OH)(2)D(3). I. Chick intestine. Am J Physiol Endocrinol Metab. 2000;278:E1104-14 pubmed
Dwivedi P, Omdahl J, Kola I, Hume D, May B. Regulation of rat cytochrome P450C24 (CYP24) gene expression. Evidence for functional cooperation of Ras-activated Ets transcription factors with the vitamin D receptor in 1,25-dihydroxyvitamin D(3)-mediated induction. J Biol Chem. 2000;275:47-55 pubmed
Michigami T, Suga A, Yamazaki M, Shimizu C, Cai G, Okada S, et al. Identification of amino acid sequence in the hinge region of human vitamin D receptor that transfers a cytosolic protein to the nucleus. J Biol Chem. 1999;274:33531-8 pubmed
Li X, Boudjelal M, Xiao J, Peng Z, Asuru A, Kang S, et al. 1,25-Dihydroxyvitamin D3 increases nuclear vitamin D3 receptors by blocking ubiquitin/proteasome-mediated degradation in human skin. Mol Endocrinol. 1999;13:1686-94 pubmed
Manfredini R, Trevisan F, Grande A, Tagliafico E, Montanari M, Lemoli R, et al. Induction of a functional vitamin D receptor in all-trans-retinoic acid-induced monocytic differentiation of M2-type leukemic blast cells. Cancer Res. 1999;59:3803-11 pubmed
Amizuka N, Kwan M, Goltzman D, Ozawa H, White J. Vitamin D3 differentially regulates parathyroid hormone/parathyroid hormone-related peptide receptor expression in bone and cartilage. J Clin Invest. 1999;103:373-81 pubmed
Morgan J, Sliney D, Morgan D, Maizel A. Differential regulation of gene transcription in subpopulations of human B lymphocytes by vitamin D3. Endocrinology. 1999;140:381-91 pubmed
Nakajima S, Yamagata M, Sakai N, Ozono K. Characterization of the activation function-2 domain of the human 1,25-dihydroxyvitamin D3 receptor. Mol Cell Endocrinol. 1998;139:15-24 pubmed
Thompson P, Jurutka P, Haussler C, Whitfield G, Haussler M. Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions. J Biol Chem. 1998;273:8483-91 pubmed
Barsony J, Renyi I, McKoy W. Subcellular distribution of normal and mutant vitamin D receptors in living cells. Studies with a novel fluorescent ligand. J Biol Chem. 1997;272:5774-82 pubmed
Mackey S, Heymont J, Kronenberg H, Demay M. Vitamin D receptor binding to the negative human parathyroid hormone vitamin D response element does not require the retinoid x receptor. Mol Endocrinol. 1996;10:298-305 pubmed
Liu S, Koszewski N, Lupez M, Malluche H, Olivera A, Russell J. Characterization of a response element in the 5'-flanking region of the avian (chicken) PTH gene that mediates negative regulation of gene transcription by 1,25-dihydroxyvitamin D3 and binds the vitamin D3 receptor. Mol Endocrinol. 1996;10:206-15 pubmed
Kremer R, Sebag M, Champigny C, Meerovitch K, Hendy G, White J, et al. Identification and characterization of 1,25-dihydroxyvitamin D3-responsive repressor sequences in the rat parathyroid hormone-related peptide gene. J Biol Chem. 1996;271:16310-6 pubmed
Ch ng J, Ibrahim B. Transcriptional and posttranscriptional mechanisms modulate creatine kinase expression during differentiation of osteoblastic cells. J Biol Chem. 1994;269:2336-41 pubmed
Morgan J, Reddy G, Uskokovic M, May B, Omdahl J, Maizel A, et al. Functional block for 1 alpha,25-dihydroxyvitamin D3-mediated gene regulation in human B lymphocytes. J Biol Chem. 1994;269:13437-43 pubmed
Ferrari S, Battini R, Molinari S. Specific binding to vitamin D response elements of chicken intestinal DNA-binding activity is not related to the vitamin D receptor. Mol Endocrinol. 1994;8:173-81 pubmed
Lasky S, Posner M, Iwata K, Santos Moore A, Yen A, Samuel V, et al. Characterization of a vitamin D3-resistant human chronic myelogenous leukemia cell line. Blood. 1994;84:4283-94 pubmed
Liu L, Ng M, Iacopino A, Dunn S, Hughes M, Bourdeau J. Vitamin D receptor gene expression in mammalian kidney. J Am Soc Nephrol. 1994;5:1251-8 pubmed
Elstner E, Linker Israeli M, Said J, Umiel T, De Vos S, Shintaku I, et al. 20-epi-vitamin D3 analogues: a novel class of potent inhibitors of proliferation and inducers of differentiation of human breast cancer cell lines. Cancer Res. 1995;55:2822-30 pubmed
Bhatia M, Kirkland J, Meckling Gill K. Monocytic differentiation of acute promyelocytic leukemia cells in response to 1,25-dihydroxyvitamin D3 is independent of nuclear receptor binding. J Biol Chem. 1995;270:15962-5 pubmed
Alroy I, Towers T, Freedman L. Transcriptional repression of the interleukin-2 gene by vitamin D3: direct inhibition of NFATp/AP-1 complex formation by a nuclear hormone receptor. Mol Cell Biol. 1995;15:5789-99 pubmed
Pike J, Donaldson C, Marion S, Haussler M. Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor. Proc Natl Acad Sci U S A. 1982;79:7719-23 pubmed
Pike J, Marion S, Donaldson C, Haussler M. Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein. J Biol Chem. 1983;258:1289-96 pubmed
Pike J. Monoclonal antibodies to chick intestinal receptors for 1,25-dihydroxyvitamin D3. Interaction and effects of binding on receptor function. J Biol Chem. 1984;259:1167-73 pubmed
Milde P, Merke J, Ritz E, Haussler M, Rauterberg E. Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods. J Histochem Cytochem. 1989;37:1609-17 pubmed
Mangelsdorf D, Pike J, Haussler M. Avian and mammalian receptors for 1,25-dihydroxyvitamin D3: in vitro translation to characterize size and hormone-dependent regulation. Proc Natl Acad Sci U S A. 1987;84:354-8 pubmed
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product information
Product Type :
Antibody
Product Name :
VDR Monoclonal Antibody (9A7)
Catalog # :
MA1-710
Quantity :
100 µg
Price :
US 430.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Rat
Reactivity :
Chicken, Human, Many, Mouse, Rat
Applications :
ChIP Assay: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL
Species :
Chicken, Human, Many, Mouse, Rat
Clone :
9A7
Isotype :
IgG2b
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.
Immunogen :
Partially purified chicken intestinal cytoplasmic 1,25-(OH)2D3 receptor protein.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL
Aliases :
1,25-dihydroxyvitamin D3 receptor; HGNC:12679; I79_025795; Nr1i1; Nuclear receptor subfamily 1 group I member 1; PPP1R163; protein phosphatase 1, regulatory subunit 163; vdr; vdr.S; vdr-A; vitamin D (1,25- dihydroxyvitamin D3) receptor; vitamin D (1,25- dihydroxyvitamin D3) receptor S homeolog; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; vitamin D receptor protein; vitamin D receptor S homeolog; Vitamin D3 receptor; XELAEV_18015993mg; xVDR
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA