Ly-6A/E Monoclonal Antibody (D7) | Invitrogen MA1-70082 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Ly-6A/E Monoclonal Antibody (D7)

catalog :

MA1-70082

quantity :

200 ug

price :

US 464.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

reactivity :

mouse

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s1b	Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher
flow cytometry; mouse; fig 6g	Stephens J, Bailey J, Hang H, Rittell V, Dietrich M, Mynatt R, et al. Adipose Tissue Dysfunction Occurs Independently of Obesity in Adipocyte-Specific Oncostatin Receptor Knockout Mice. Obesity (Silver Spring). 2018;26:1439-1447 pubmed publisher
flow cytometry; mouse; loading ...; fig s1e	Baumgartner C, Toifl S, Farlik M, Halbritter F, Scheicher R, Fischer I, et al. An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion. Cell Stem Cell. 2018;22:879-892.e6 pubmed publisher
flow cytometry; mouse; loading ...; fig 4g	Huang Y, Rajappa P, Hu W, Hoffman C, CISSE B, Kim J, et al. A proangiogenic signaling axis in myeloid cells promotes malignant progression of glioma. J Clin Invest. 2017;127:1826-1838 pubmed publisher
flow cytometry; mouse; fig 1a	Bruce D, Stefanski H, Vincent B, Dant T, Reisdorf S, Bommiasamy H, et al. Type 2 innate lymphoid cells treat and prevent acute gastrointestinal graft-versus-host disease. J Clin Invest. 2017;127:1813-1825 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c,d	Guidi N, Sacma M, Ständker L, Soller K, Marka G, Eiwen K, et al. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells. EMBO J. 2017;36:840-853 pubmed publisher
flow cytometry; mouse; loading ...; fig 4a	Gopinath S. Inhibition of Stat3 signaling ameliorates atrophy of the soleus muscles in mice lacking the vitamin D receptor. Skelet Muscle. 2017;7:2 pubmed publisher
flow cytometry; mouse; loading ...; fig 2c	Hattori A, McSkimming D, Kannan N, Ito T. RNA binding protein MSI2 positively regulates FLT3 expression in myeloid leukemia. Leuk Res. 2017;54:47-54 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Cañete A, Carmona R, Ariza L, Sanchez M, Rojas A, Muñoz Chápuli R. A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice. Haematologica. 2017;102:647-655 pubmed publisher
flow cytometry; mouse; loading ...	Le Q, Yao W, Chen Y, Yan B, Liu C, Yuan M, et al. GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal. Cell Death Dis. 2016;7:e2478 pubmed publisher
flow cytometry; mouse; loading ...	Ramirez Carrozzi V, Sambandam A, Zhou M, Yan D, Kang J, Wu X, et al. Combined blockade of the IL-13 and IL-33 pathways leads to a greater inhibition of type 2 inflammation over inhibition of either pathway alone. J Allergy Clin Immunol. 2017;139:705-708.e6 pubmed publisher
flow cytometry; mouse; loading ...; fig st1	Johnston L, Hsu C, Krier Burris R, Chhiba K, Chien K, McKenzie A, et al. IL-33 Precedes IL-5 in Regulating Eosinophil Commitment and Is Required for Eosinophil Homeostasis. J Immunol. 2016;197:3445-3453 pubmed
flow cytometry; mouse	Henry E, Sy C, Inclan Rico J, Espinosa V, Ghanny S, Dwyer D, et al. Carbonic anhydrase enzymes regulate mast cell-mediated inflammation. J Exp Med. 2016;213:1663-73 pubmed publisher
flow cytometry; mouse; loading ...; fig ex1b	Hoppe P, Schwarzfischer M, Loeffler D, Kokkaliaris K, Hilsenbeck O, Moritz N, et al. Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios. Nature. 2016;535:299-302 pubmed publisher
flow cytometry; mouse; fig s1e	Yu Q, Song W, Wang D, Zeng Y. Identification of blood vascular endothelial stem cells by the expression of protein C receptor. Cell Res. 2016;26:1079-1098 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile. J Immunol. 2016;196:4760-70 pubmed publisher
flow cytometry; mouse; loading ...	Leiva M, Quintana J, Ligos J, Hidalgo A. Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGFβ availability. Nat Commun. 2016;7:10222 pubmed publisher
flow cytometry; mouse; fig 2	Kaplan J, Marshall M, C McSkimming C, Harmon D, Garmey J, Oldham S, et al. Adipocyte progenitor cells initiate monocyte chemoattractant protein-1-mediated macrophage accumulation in visceral adipose tissue. Mol Metab. 2015;4:779-94 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Zhong C, Cui K, Wilhelm C, Hu G, Mao K, Belkaid Y, et al. Group 3 innate lymphoid cells continuously require the transcription factor GATA-3 after commitment. Nat Immunol. 2016;17:169-78 pubmed publisher
flow cytometry; mouse; fig 1	Xu G, Wu H, Zhang J, Li D, Wang Y, Wang Y, et al. Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice. Free Radic Biol Med. 2015;87:15-25 pubmed publisher
flow cytometry; mouse; 1:500; loading ...	Khaled W, Choon Lee S, Stingl J, Chen X, Raza Ali H, Rueda O, et al. BCL11A is a triple-negative breast cancer gene with critical functions in stem and progenitor cells. Nat Commun. 2015;6:5987 pubmed publisher
flow cytometry; mouse	Powell N, Walker A, Stolarczyk E, Canavan J, Gökmen M, Marks E, et al. The transcription factor T-bet regulates intestinal inflammation mediated by interleukin-7 receptor+ innate lymphoid cells. Immunity. 2012;37:674-84 pubmed publisher
western blot; mouse; fig 3	Palfree R, Dumont F, Hammerling U. Ly-6A.2 and Ly-6E.1 molecules are antithetical and identical to MALA-1. Immunogenetics. 1986;23:197-207 pubmed
flow cytometry; mouse; fig 1	Codias E, Cray C, Baler R, Levy R, Malek T. Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes. Immunogenetics. 1989;29:98-107 pubmed
	Sokal I, Hu G, Liang Y, Mao M, Wensel T, Palczewski K. Identification of protein kinase C isozymes responsible for the phosphorylation of photoreceptor-specific RGS9-1 at Ser475. J Biol Chem. 2003;278:8316-25 pubmed
	He W, Melia T, Cowan C, Wensel T. Dependence of RGS9-1 membrane attachment on its C-terminal tail. J Biol Chem. 2001;276:48961-6 pubmed
	Hu G, Jang G, Cowan C, Wensel T, Palczewski K. Phosphorylation of RGS9-1 by an endogenous protein kinase in rod outer segments. J Biol Chem. 2001;276:22287-95 pubmed

product information

Product Type :

Antibody

Product Name :

Ly-6A/E Monoclonal Antibody (D7)

Catalog # :

MA1-70082

Quantity :

200 ug

Price :

US 464.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 1 ug/1 million cells, Immunocytochemistry: 5 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse

Clone :

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.

Immunogen :

IL-2-dependent mouse T-cell line CTL-L.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 ug/1 million cells, Immunocytochemistry: 5 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1

more info or order :

Invitrogen product webpage