I-Ab Monoclonal Antibody (28-16-8S) | Invitrogen MA1-70064 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

I-Ab Monoclonal Antibody (28-16-8S)

catalog :

MA1-70064

quantity :

250 µg

price :

US 376

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

28-16-8s

reactivity :

mouse

application :

immunohistochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Ferret Bernard S, Castro Borges W, Dowle A, Sanin D, Cook P, Turner J, et al. Plasma membrane proteomes of differentially matured dendritic cells identified by LC-MS/MS combined with iTRAQ labelling. J Proteomics. 2012;75:938-48 pubmed publisher
flow cytometry; mouse; fig 4	Wells J, Cowled C, Farzaneh F, Noble A. Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity. J Immunol. 2008;181:3422-31 pubmed
flow cytometry; mouse; fig 6	Hewitson J, Jenkins G, Hamblin P, Mountford A. CD40/CD154 interactions are required for the optimal maturation of skin-derived APCs and the induction of helminth-specific IFN-gamma but not IL-4. J Immunol. 2006;177:3209-17 pubmed
flow cytometry; mouse	Jenkins S, Mountford A. Dendritic cells activated with products released by schistosome larvae drive Th2-type immune responses, which can be inhibited by manipulation of CD40 costimulation. Infect Immun. 2005;73:395-402 pubmed
flow cytometry; mouse	Morin J, Faideau B, Gagnerault M, Lepault F, Boitard C, Boudaly S. Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice. Clin Exp Immunol. 2003;134:388-95 pubmed
flow cytometry; mouse; fig 1 immunohistochemistry; mouse; fig 2	Morin J, Chimènes A, Boitard C, Berthier R, Boudaly S. Granulocyte-dendritic cell unbalance in the non-obese diabetic mice. Cell Immunol. 2003;223:13-25 pubmed

product information

Product Type :

Antibody

Product Name :

I-Ab Monoclonal Antibody (28-16-8S)

Catalog # :

MA1-70064

Quantity :

250 µg

Price :

US 376

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Mouse

Applications :

Flow Cytometry: 1 µg/1 million cells

Species :

Mouse

Clone :

28-16-8S

Isotype :

IgM

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

I-Ab (H2-Ab1, histocompatibility 2, class II antigen A, beta 1) is an MHC class II heterodimer molecule of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. H2-Ab1 heterodimer chains alpha (DQA) and beta (DQB) are both anchored in the membrane. H2-Ab1 plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for polymorphisms is routinely done for bone marrow transplantation. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Immunogen :

C3H.SW spleen.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/1 million cells

Aliases :

Abeta; AI845868; CELIAC1; DADB-249P12.2; H-2 class II histocompatibility antigen, A beta chain; H-2Ab; H2-Ab; H2-Ab1; H2Eb; H-2Eb; H2-iabeta; histocompatibility 2, class II antigen A, beta 1; HLA-DQB; Ia2; Ia-2; Ia4; Ia-4; IAb; I-Abeta; IDDM1; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1; Rmcs1

more info or order :

Invitrogen product webpage