Prolactin Receptor Monoclonal Antibody (U5) | Invitrogen MA1-610 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Prolactin Receptor Monoclonal Antibody (U5)

catalog :

MA1-610

quantity :

100 ug

price :

US 471.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

African green monkey, hamsters, human, mouse, rat, bovine, pigs , domestic rabbit, domestic horse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 49

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; bovine; 1:50; fig 5	Lebedeva I, Singina G, Lopukhov A, Shedova E, Zinovieva N. Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways. Front Genet. 2015;6:274 pubmed publisher
immunocytochemistry; bovine; 1:50	Lebedeva I, Singina G, Volkova N, Vejlsted M, Zinovieva N, Schmidt M. Prolactin affects bovine oocytes through direct and cumulus-mediated pathways. Theriogenology. 2014;82:1154-64 pubmed publisher
western blot; hamsters; 1:1000 immunohistochemistry; human; 1:33	Belugin S, Diogenes A, Patil M, Ginsburg E, Henry M, Akopian A. Mechanisms of transient signaling via short and long prolactin receptor isoforms in female and male sensory neurons. J Biol Chem. 2013;288:34943-55 pubmed publisher
immunohistochemistry; domestic rabbit; 1:75	Sakr O, Garcia Garcia R, Arias Alvarez M, Millan P, Lorenzo P, Rebollar P. Body reserves and ovarian performance in primiparous lactating rabbit does submitted to early weaning as a strategy to decrease energy deficit. Anim Reprod Sci. 2010;121:294-300 pubmed publisher
immunohistochemistry; rat; 1:1000	Tejadilla D, Cerbon M, Morales T. Prolactin reduces the damaging effects of excitotoxicity in the dorsal hippocampus of the female rat independently of ovarian hormones. Neuroscience. 2010;169:1178-85 pubmed publisher
western blot; human	Reuwer A, Nieuwland R, Fernandez I, Goffin V, Van Tiel C, Schaap M, et al. Prolactin does not affect human platelet aggregation or secretion. Thromb Haemost. 2009;101:1119-27 pubmed
immunohistochemistry; human; 3 ug/ml western blot; human	Galsgaard E, Rasmussen B, Folkesson C, Rasmussen L, Berchtold M, Christensen L, et al. Re-evaluation of the prolactin receptor expression in human breast cancer. J Endocrinol. 2009;201:115-28 pubmed publisher
immunohistochemistry; rat; 10 ug/ml	Asai S, Ohta R, Fujikawa T, Sakai R, Shirota M, Ogata M, et al. Gastric ulceration and expression of prolactin receptor in the brain in Hatano high- and low-avoidance rats. Endocrine. 2006;30:161-6 pubmed
immunohistochemistry; rat; 1:200	Möderscheim T, Gorba T, Pathipati P, Kokay I, Grattan D, Williams C, et al. Prolactin is involved in glial responses following a focal injury to the juvenile rat brain. Neuroscience. 2007;145:963-73 pubmed
western blot; African green monkey	Nien W, Dauphinee S, Moffat L, Too C. Overexpression of the mTOR alpha4 phosphoprotein activates protein phosphatase 2A and increases Stat1alpha binding to PIAS1. Mol Cell Endocrinol. 2007;263:10-7 pubmed
western blot; rat; 1:2000	Anhê G, Torrão A, Nogueira T, Caperuto L, Amaral M, Medina M, et al. ERK3 associates with MAP2 and is involved in glucose-induced insulin secretion. Mol Cell Endocrinol. 2006;251:33-41 pubmed
immunohistochemistry; rat; 20 ug/ml	Kolonin M, Sun J, Do K, Vidal C, Ji Y, Baggerly K, et al. Synchronous selection of homing peptides for multiple tissues by in vivo phage display. FASEB J. 2006;20:979-81 pubmed
	Yao D, Lu Y, Li L, Wang S, Mu Y, Ding C, et al. Prolactin and glucocorticoid receptors in the prefrontal cortex are associated with anxiety-like behavior in prenatally stressed adolescent offspring rats. J Neuroendocrinol. 2023;35:e13231 pubmed publisher
	Garza Rodr xed guez M, Rodr xed guez Sanchez I, Gonz xe1 lez xc1 lvarez R, Luna M, Burciaga Flores C, Alcorta Nu xf1 ez F, et al. Prolactin Expression in the Baboon (Papio hamadryas) Eye. Animals (Basel). 2022;12: pubmed publisher
	Roach C, Bidne K, Romoser M, Ross J, Baumgard L, Keating A. Impact of heat stress on prolactin-mediated ovarian JAK-STAT signaling in postpubertal gilts. J Anim Sci. 2022;100: pubmed publisher
	Mellado S, Moreno Ruiz B, Exp xf3 sito S, Fern xe1 ndez M, Mart xed n E. Prolactin Reduces Hippocampal Parvalbumin and GABAA Receptor Expression in Female Mice. Neuroendocrinology. 2022;112:796-806 pubmed publisher
	Torner L, Karg S, Blume A, Kandasamy M, Kuhn H, Winkler J, et al. Prolactin prevents chronic stress-induced decrease of adult hippocampal neurogenesis and promotes neuronal fate. J Neurosci. 2009;29:1826-33 pubmed publisher
	Blume A, Torner L, Liu Y, Subburaju S, Aguilera G, Neumann I. Prolactin activates mitogen-activated protein kinase signaling and corticotropin releasing hormone transcription in rat hypothalamic neurons. Endocrinology. 2009;150:1841-9 pubmed publisher
	Diogenes A, Patwardhan A, Jeske N, Ruparel N, Goffin V, Akopian A, et al. Prolactin modulates TRPV1 in female rat trigeminal sensory neurons. J Neurosci. 2006;26:8126-36 pubmed
	Dong J, Tsai Morris C, Dufau M. A novel estradiol/estrogen receptor alpha-dependent transcriptional mechanism controls expression of the human prolactin receptor. J Biol Chem. 2006;281:18825-36 pubmed
	Fujikawa T, Tamura K, Kawase T, Mori Y, Sakai R, Sakuma K, et al. Prolactin receptor knockdown in the rat paraventricular nucleus by a morpholino-antisense oligonucleotide causes hypocalcemia and stress gastric erosion. Endocrinology. 2005;146:3471-80 pubmed
	Ma F, Grattan D, Goffin V, Bunn S. Prolactin-regulated tyrosine hydroxylase activity and messenger ribonucleic acid expression in mediobasal hypothalamic cultures: the differential role of specific protein kinases. Endocrinology. 2005;146:93-102 pubmed
	Corbacho A, Valacchi G, Kubala L, Olano Martin E, Schock B, Kenny T, et al. Tissue-specific gene expression of prolactin receptor in the acute-phase response induced by lipopolysaccharides. Am J Physiol Endocrinol Metab. 2004;287:E750-7 pubmed
	Li Y, Kumar K, Tang W, Spiegelman V, Fuchs S. Negative regulation of prolactin receptor stability and signaling mediated by SCF(beta-TrCP) E3 ubiquitin ligase. Mol Cell Biol. 2004;24:4038-48 pubmed
	Fujikawa T, Soya H, Tamashiro K, Sakai R, McEwen B, Nakai N, et al. Prolactin prevents acute stress-induced hypocalcemia and ulcerogenesis by acting in the brain of rat. Endocrinology. 2004;145:2006-13 pubmed
	Augustine R, Kokay I, Andrews Z, Ladyman S, Grattan D. Quantitation of prolactin receptor mRNA in the maternal rat brain during pregnancy and lactation. J Mol Endocrinol. 2003;31:221-32 pubmed
	Abkowitz J, Schaison G, Boulad F, Brown D, Buchanan G, Johnson C, et al. Response of Diamond-Blackfan anemia to metoclopramide: evidence for a role for prolactin in erythropoiesis. Blood. 2002;100:2687-91 pubmed
	Fresno Vara J, Caceres M, Silva A, Martin Perez J. Src family kinases are required for prolactin induction of cell proliferation. Mol Biol Cell. 2001;12:2171-83 pubmed
	Pi X, Voogt J. Mechanisms for suckling-induced changes in expression of prolactin receptor in the hypothalamus of the lactating rat. Brain Res. 2001;891:197-205 pubmed
	Ling C, Hellgren G, Gebre Medhin M, Dillner K, Wennbo H, Carlsson B, et al. Prolactin (PRL) receptor gene expression in mouse adipose tissue: increases during lactation and in PRL-transgenic mice. Endocrinology. 2000;141:3564-72 pubmed
	Yamauchi T, Yamauchi N, Ueki K, Sugiyama T, Waki H, Miki H, et al. Constitutive tyrosine phosphorylation of ErbB-2 via Jak2 by autocrine secretion of prolactin in human breast cancer. J Biol Chem. 2000;275:33937-44 pubmed
	Pi X, Voogt J. Effect of suckling on prolactin receptor immunoreactivity in the hypothalamus of the rat. Neuroendocrinology. 2000;71:308-17 pubmed
	Cheng Y, Zhizhin I, Perlman R, Mangoura D. Prolactin-induced cell proliferation in PC12 cells depends on JNK but not ERK activation. J Biol Chem. 2000;275:23326-32 pubmed
	Melck D, De Petrocellis L, Orlando P, Bisogno T, Laezza C, Bifulco M, et al. Suppression of nerve growth factor Trk receptors and prolactin receptors by endocannabinoids leads to inhibition of human breast and prostate cancer cell proliferation. Endocrinology. 2000;141:118-26 pubmed
	Morales P, Carretero M, Geronimo H, Copin S, Gaspar M, Marcos M, et al. Influence of prolactin on the differentiation of mouse B-lymphoid precursors. Cell Growth Differ. 1999;10:583-90 pubmed
	Pi X, Grattan D. Increased prolactin receptor immunoreactivity in the hypothalamus of lactating rats. J Neuroendocrinol. 1999;11:693-705 pubmed
	De Petrocellis L, Melck D, Palmisano A, Bisogno T, Laezza C, Bifulco M, et al. The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation. Proc Natl Acad Sci U S A. 1998;95:8375-80 pubmed
	Perrot Applanat M, Gualillo O, Buteau H, Edery M, Kelly P. Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation. J Cell Sci. 1997;110 ( Pt 9):1123-32 pubmed
	Ormandy C, Camus A, Barra J, Damotte D, Lucas B, Buteau H, et al. Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouse. Genes Dev. 1997;11:167-78 pubmed
	Gagnerault M, Touraine P, Savino W, Kelly P, Dardenne M. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol. 1993;150:5673-81 pubmed
	Rui H, Lebrun J, Kirken R, Kelly P, Farrar W. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology. 1994;135:1299-306 pubmed
	Clevenger C, Ngo W, Sokol D, Luger S, Gewirtz A. Vav is necessary for prolactin-stimulated proliferation and is translocated into the nucleus of a T-cell line. J Biol Chem. 1995;270:13246-53 pubmed
	Royster M, Driscoll P, Kelly P, Freemark M. The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation. Endocrinology. 1995;136:3892-900 pubmed
	Waters M, Daniel N, Bignon C, Djiane J. The rabbit mammary gland prolactin receptor is tyrosine-phosphorylated in response to prolactin in vivo and in vitro. J Biol Chem. 1995;270:5136-43 pubmed
	Okamura H, Raguet S, Bell A, Gagnon J, Kelly P. Purification and protein sequence analysis of rat liver prolactin receptor. J Biol Chem. 1989;264:5904-11 pubmed
	Okamura H, Zachwieja J, Raguet S, Kelly P. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endocrinology. 1989;124:2499-508 pubmed
	Elberg G, Kelly P, Djiane J, Binder L, Gertler A. Mitogenic and binding properties of monoclonal antibodies to the prolactin receptor in Nb2 rat lymphoma cells. Selective enhancement by anti-mouse IgG. J Biol Chem. 1990;265:14770-6 pubmed
	Dardenne M, Kelly P, Bach J, Savino W. Identification and functional activity of prolactin receptors in thymic epithelial cells. Proc Natl Acad Sci U S A. 1991;88:9700-4 pubmed
	Rozakis Adcock M, Kelly P. Mutational analysis of the ligand-binding domain of the prolactin receptor. J Biol Chem. 1991;266:16472-7 pubmed

product information

Product Type :

Antibody

Product Name :

Prolactin Receptor Monoclonal Antibody (U5)

Catalog # :

MA1-610

Quantity :

100 ug

Price :

US 471.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Equine, Human, Mouse, Porcine, Rabbit, Rat

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 ug/mL

Species :

Equine, Human, Mouse, Porcine, Rabbit, Rat

Clone :

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Immunogen :

Purified rat liver PRL receptor.

Format :

Lyophilized

Applications w/Dilutions :

Aliases :

AI987712; cytokine receptor; HPRL; hPRL receptor; hPRLrI; Lactogen receptor; MFAB; pPRLR-SF; Pr-1; Pr-3; Prl R; PRLR; PRL-R; Prlr-rs1; Prolactin receptor; prolactin receptor 2 precursor; prolactin receptor related sequence 1; prolactin receptor short isoform; RATPRLR; secreted prolactin binding protein

more info or order :

Invitrogen product webpage