HEF1 Monoclonal Antibody (2G9) | Invitrogen MA1-5785 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HEF1 Monoclonal Antibody (2G9)

catalog :

MA1-5785

quantity :

100 uL

price :

US 738.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G9

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000	Bosch R, Dieguez Gonzalez R, Céspedes M, Parreño M, Pavon M, Granena A, et al. A novel inhibitor of focal adhesion signaling induces caspase-independent cell death in diffuse large B-cell lymphoma. Blood. 2011;118:4411-20 pubmed publisher
	Zeinieh M, Salehi A, Rajkumar V, Barker P. p75NTR-dependent Rac1 activation requires receptor cleavage and activation of an NRAGE and NEDD9 signaling cascade. J Cell Sci. 2015;128:447-59 pubmed

product information

Product Type :

Antibody

Product Name :

HEF1 Monoclonal Antibody (2G9)

Catalog # :

MA1-5785

Quantity :

100 uL

Price :

US 738.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1:2,000-1:6,000

Species :

Human, Mouse, Rat

Clone :

2G9

Isotype :

IgG1, kappa

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.

Immunogen :

Fusion protein, corresponding to amino acids 82-398 of Human HEF1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1:2,000-1:6,000

Aliases :

Cas scaffolding protein family member 2; CAS2; CASL; CAS-L; cas-like docking; CASS2; Crk-associated substrate lymphocyte-specific protein; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF1; mEF1; Nedd9; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; neural precursor cell expressed, developmentally down-regulated gene 9; p105; p130Cas-related protein; renal carcinoma antigen NY-REN-12

more info or order :

Invitrogen product webpage