HIF1A Monoclonal Antibody (mgc3) | Invitrogen MA1-516 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HIF1A Monoclonal Antibody (mgc3)

catalog :

MA1-516

quantity :

100 uL

price :

US 562.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mgc3

reactivity :

human, mouse, rat, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, immunohistochemistry - paraffin section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 44

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:200; fig s1a	Omatsu Kanbe M, Nozuchi N, Nishino Y, Mukaisho K, Sugihara H, Matsuura H. Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death. Sci Rep. 2017;7:41318 pubmed publisher
western blot; human; loading ...; fig 1a	Higashimura Y, Kitakaze T, Harada N, Inui H, Nakano Y, Yamaji R. pVHL-mediated degradation of HIF-2? regulates estrogen receptor ? expression in normoxic breast cancer cells. FEBS Lett. 2016;590:2690-9 pubmed publisher
EMSA; mouse EMSA; rat	Kulzer J, Stitzel M, Morken M, Huyghe J, Fuchsberger C, Kuusisto J, et al. A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell. Am J Hum Genet. 2014;94:186-97 pubmed publisher
immunohistochemistry; human; 1:50 western blot; human; 1:2000	Mandl M, Kapeller B, Lieber R, Macfelda K. Hypoxia-inducible factor-1? (HIF-1?) is upregulated in a HIF-1?-dependent manner in 518A2 human melanoma cells under hypoxic conditions. Biochem Biophys Res Commun. 2013;434:166-72 pubmed publisher
western blot; rat; 1:1500	Ma Y, Lovekamp Swan T, Bekele W, Dohi A, Schreihofer D. Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats. Endocrinology. 2013;154:1589-97 pubmed publisher
blocking or activating experiments; mouse	Jin J, Sison K, Li C, Tian R, Wnuk M, Sung H, et al. Soluble FLT1 binds lipid microdomains in podocytes to control cell morphology and glomerular barrier function. Cell. 2012;151:384-99 pubmed publisher
western blot; mouse; 1:500	Croci D, Salatino M, Rubinstein N, Cerliani J, Cavallin L, Leung H, et al. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma. J Exp Med. 2012;209:1985-2000 pubmed publisher
immunoprecipitation; human immunohistochemistry; human western blot; human	Mitani T, Harada N, Nakano Y, Inui H, Yamaji R. Coordinated action of hypoxia-inducible factor-1? and ?-catenin in androgen receptor signaling. J Biol Chem. 2012;287:33594-606 pubmed
western blot; human	Higashimura Y, Nakajima Y, Yamaji R, Harada N, Shibasaki F, Nakano Y, et al. Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells. Arch Biochem Biophys. 2011;509:1-8 pubmed publisher
immunocytochemistry; human; 1:500 western blot; human; 1:2000	Mitani T, Yamaji R, Higashimura Y, Harada N, Nakano Y, Inui H. Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1? in a low androgen environment. J Steroid Biochem Mol Biol. 2011;123:58-64 pubmed publisher
western blot; bovine; 1:2,000	Xing D, Bonanno J. Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF. Mol Vis. 2009;15:1020-7 pubmed
western blot; human	Kokkonen N, Ulibarri I, Kauppila A, Luosujärvi H, Rivinoja A, Pospiech H, et al. Hypoxia upregulates carcinoembryonic antigen expression in cancer cells. Int J Cancer. 2007;121:2443-50 pubmed
immunoprecipitation; human immunohistochemistry; human; 1:50 western blot; human; 1:250	Ietta F, Wu Y, Winter J, Xu J, Wang J, Post M, et al. Dynamic HIF1A regulation during human placental development. Biol Reprod. 2006;75:112-21 pubmed
western blot; bovine	Xing D, Sun X, Li J, Cui M, Tan Allen K, Bonanno J. Hypoxia preconditioning protects corneal stromal cells against induced apoptosis. Exp Eye Res. 2006;82:780-7 pubmed
western blot; mouse; 1:100	van Tuyl M, Liu J, Wang J, Kuliszewski M, Tibboel D, Post M. Role of oxygen and vascular development in epithelial branching morphogenesis of the developing mouse lung. Am J Physiol Lung Cell Mol Physiol. 2005;288:L167-78 pubmed
	Yang Y, Li Z, Wang Y, Gao J, Meng Y, Wang S, et al. The regulatory relationship between NAMPT and PD-L1 in cancer and identification of a dual-targeting inhibitor. EMBO Mol Med. 2024;16:885-903 pubmed publisher
	Wittig F, Koch F, Pannenberg L, Bekeschus S, Ramer R, Hinz B. β-Caryophyllene Inhibits Endothelial Tube Formation by Modulating the Secretome of Hypoxic Lung Cancer Cells-Possible Role of VEGF Downregulation. Int J Mol Sci. 2024;25: pubmed publisher
	DeFrates K, Tong E, Cheng J, Heber Katz E, Messersmith P. A Pro-Regenerative Supramolecular Prodrug Protects Against and Repairs Colon Damage in Experimental Colitis. Adv Sci (Weinh). 2024;11:e2304716 pubmed publisher
	Wittig F, Pannenberg L, Schwarz R, Bekeschus S, Ramer R, Hinz B. Antiangiogenic Action of JZL184 on Endothelial Cells via Inhibition of VEGF Expression in Hypoxic Lung Cancer Cells. Cells. 2023;12: pubmed publisher
	Lee A, Lee S, Choi J, Um I, Na H, Lee J, et al. Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model. Exp Mol Med. 2023;55:1713-1719 pubmed publisher
	Lin Y, Lee S, Cheng Y. Low-Level Laser Therapy Induces Melanoma Tumor Growth by Promoting Angiogenesis. Life (Basel). 2023;13: pubmed publisher
	Chee N, Carriere C, Miller Z, Welford S, Brothers S. Activating transcription factor 4 regulates hypoxia inducible factor 1α in chronic hypoxia in pancreatic cancer cells. Oncol Rep. 2023;49: pubmed publisher
	Lee S, Lee A, Choi J, Lee C, Cho K, Lee J, et al. IL-17 Induces Autophagy Dysfunction to Promote Inflammatory Cell Death and Fibrosis in Keloid Fibroblasts via the STAT3 and HIF-1α Dependent Signaling Pathways. Front Immunol. 2022;13:888719 pubmed publisher
	Gao K, Zong H, Hou K, Zhang Y, Zhang R, Zhao D, et al. p53N236S Activates Autophagy in Response to Hypoxic Stress Induced by DFO. Genes (Basel). 2022;13: pubmed publisher
	Bannoud N, Garc xed a P, Gambarte Tudela J, Sundblad V, Cagnoni A, Bach C, et al. Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis. Methods Mol Biol. 2022;2442:635-653 pubmed publisher
	Santocildes G, Viscor G, Pag xe8 s T, Ramos Romero S, Torres J, Torrella J. Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats. Front Physiol. 2021;12:673095 pubmed publisher
	Gauthier L, Saati M, Bensalah Pigeon H, Ben M Barek K, Gitton Quent O, Bertrand R, et al. The HIF1α/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation. Sci Rep. 2020;10:18742 pubmed publisher
	Chen S, Pan M, Tang J, Cheng J, Zhao D, Zhang Y, et al. Arginine is neuroprotective through suppressing HIF-1α/LDHA-mediated inflammatory response after cerebral ischemia/reperfusion injury. Mol Brain. 2020;13:63 pubmed publisher
	Yao M, Rabbani Z, Sattler T, Nguyen K, Zaharoff D, Walker G, et al. Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia. Anal Chem. 2019;91:15032-15039 pubmed publisher
	Marrocco I, Altieri F, Rubini E, Paglia G, Chichiarelli S, Giamogante F, et al. Shmt2: A Stat3 Signaling New Player in Prostate Cancer Energy Metabolism. Cells. 2019;8: pubmed publisher
	Laws M, Bonomi R, Kamal S, Gelovani D, Llaniguez J, Potukutchi S, et al. Molecular imaging HDACs class IIa expression-activity and pharmacologic inhibition in intracerebral glioma models in rats using PET/CT/(MRI) with [18F]TFAHA. Sci Rep. 2019;9:3595 pubmed publisher
	Wu J, Xu W, Wei Y, Qu Y, Zhang H, Ye D. An Integrated Score and Nomogram Combining Clinical and Immunohistochemistry Factors to Predict High ISUP Grade Clear Cell Renal Cell Carcinoma. Front Oncol. 2018;8:634 pubmed publisher
	Yao M, Sattler T, Rabbani Z, Pulliam T, Walker G, Gamcsik M. Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate. Am J Physiol Cell Physiol. 2018;315:C766-C775 pubmed publisher
	Rubini E, Altieri F, Chichiarelli S, Giamogante F, Carissimi S, Paglia G, et al. STAT3, a Hub Protein of Cellular Signaling Pathways, Is Triggered by β-Hexaclorocyclohexane. Int J Mol Sci. 2018;19: pubmed publisher
	Wendeln A, Degenhardt K, Kaurani L, Gertig M, Ulas T, Jain G, et al. Innate immune memory in the brain shapes neurological disease hallmarks. Nature. 2018;556:332-338 pubmed publisher
	Ferreira J, Fôfo H, Bejarano E, Bento C, Ramalho J, Girao H, et al. STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy. Autophagy. 2013;9:1349-66 pubmed publisher
	Rathman Josserand M, Genty G, Lecardonnel J, Chabane S, Cousson A, François Michelet J, et al. Human hair follicle stem/progenitor cells express hypoxia markers. J Invest Dermatol. 2013;133:2094-7 pubmed publisher
	Higashimura Y, Terai T, Yamaji R, Mitani T, Ogawa M, Harada N, et al. Kelch-like 20 up-regulates the expression of hypoxia-inducible factor-2? through hypoxia- and von Hippel-Lindau tumor suppressor protein-independent regulatory mechanisms. Biochem Biophys Res Commun. 2011;413:201-5 pubmed publisher
	Zamudio S, Wu Y, Ietta F, Rolfo A, Cross A, Wheeler T, et al. Human placental hypoxia-inducible factor-1alpha expression correlates with clinical outcomes in chronic hypoxia in vivo. Am J Pathol. 2007;170:2171-9 pubmed
	Bayer I, Caniggia I, Adamson S, Langille B. Experimental angiogenesis of arterial vasa vasorum. Cell Tissue Res. 2002;307:303-13 pubmed
	Fernandez A, Udagawa T, Schwesinger C, Beecken W, Achilles Gerte E, McDonnell T, et al. Angiogenic potential of prostate carcinoma cells overexpressing bcl-2. J Natl Cancer Inst. 2001;93:208-13 pubmed
	Wanner R, Spielmann P, Stroka D, Camenisch G, Camenisch I, Scheid A, et al. Epolones induce erythropoietin expression via hypoxia-inducible factor-1 alpha activation. Blood. 2000;96:1558-65 pubmed
	Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, et al. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha. FASEB J. 1999;13:81-8 pubmed
	Wenger R, Kvietikova I, Rolfs A, Camenisch G, Gassmann M. Oxygen-regulated erythropoietin gene expression is dependent on a CpG methylation-free hypoxia-inducible factor-1 DNA-binding site. Eur J Biochem. 1998;253:771-7 pubmed

product information

Product Type :

Antibody

Product Name :

HIF1A Monoclonal Antibody (mgc3)

Catalog # :

MA1-516

Quantity :

100 uL

Price :

US 562.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Non-human primate, Porcine

Applications :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:20-1:200, Immunoelectrophoresis: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:2,000

Species :

Bovine, Human, Mouse, Non-human primate, Porcine

Clone :

mgc3

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Immunogen :

Human HIF-1 alpha amino acids 530-826.

Format :

Liquid

Applications w/Dilutions :

Aliases :

AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

more info or order :

Invitrogen product webpage