HIF-1 beta Monoclonal Antibody (2B10) | Invitrogen MA1-515 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HIF-1 beta Monoclonal Antibody (2B10)

catalog :

MA1-515

quantity :

100 uL

price :

US 451.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B10

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig s6a	Brigidi G, Hayes M, Delos Santos N, Hartzell A, Texari L, Lin P, et al. Genomic Decoding of Neuronal Depolarization by Stimulus-Specific NPAS4 Heterodimers. Cell. 2019;179:373-391.e27 pubmed publisher
EMSA; rat EMSA; mouse	Kulzer J, Stitzel M, Morken M, Huyghe J, Fuchsberger C, Kuusisto J, et al. A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell. Am J Hum Genet. 2014;94:186-97 pubmed publisher
western blot; mouse	Hao N, Bhakti V, Peet D, Whitelaw M. Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation. Nucleic Acids Res. 2013;41:5626-38 pubmed publisher
western blot; rat; 1:2000	Ma Y, Lovekamp Swan T, Bekele W, Dohi A, Schreihofer D. Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats. Endocrinology. 2013;154:1589-97 pubmed publisher
western blot; human	Fukushima K, Tsukimori K, Li D, Takao T, Morokuma S, Kato K, et al. Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines. Hum Exp Toxicol. 2012;31:550-6 pubmed publisher
western blot; human	Hao N, Whitelaw M, Shearwin K, Dodd I, Chapman Smith A. Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR. Nucleic Acids Res. 2011;39:3695-709 pubmed publisher
immunohistochemistry; human	Céspedes M, Galindo M, Couso J. Dioxin toxicity in vivo results from an increase in the dioxin-independent transcriptional activity of the aryl hydrocarbon receptor. PLoS ONE. 2010;5:e15382 pubmed publisher
western blot; mouse	Whelan F, Hao N, Furness S, Whitelaw M, Chapman Smith A. Amino acid substitutions in the aryl hydrocarbon receptor ligand binding domain reveal YH439 as an atypical AhR activator. Mol Pharmacol. 2010;77:1037-46 pubmed publisher
western blot; human	Murray I, Morales J, Flaveny C, Dinatale B, Chiaro C, Gowdahalli K, et al. Evidence for ligand-mediated selective modulation of aryl hydrocarbon receptor activity. Mol Pharmacol. 2010;77:247-54 pubmed publisher
immunoprecipitation; zebrafish western blot; zebrafish ; 1:4,000 immunoprecipitation; human western blot; human; 1:4,000	Evans B, Karchner S, Allan L, Pollenz R, Tanguay R, Jenny M, et al. Repression of aryl hydrocarbon receptor (AHR) signaling by AHR repressor: role of DNA binding and competition for AHR nuclear translocator. Mol Pharmacol. 2008;73:387-98 pubmed
	Karchner S, Jenny M, Tarrant A, Evans B, Kang H, Bae I, et al. The active form of human aryl hydrocarbon receptor (AHR) repressor lacks exon 8, and its Pro 185 and Ala 185 variants repress both AHR and hypoxia-inducible factor. Mol Cell Biol. 2009;29:3465-77 pubmed publisher
	Rowatt A, DePowell J, Powell W. ARNT gene multiplicity in amphibians: characterization of ARNT2 from the frog Xenopus laevis. J Exp Zool B Mol Dev Evol. 2003;300:48-57 pubmed
	Elbi C, Misteli T, Hager G. Recruitment of dioxin receptor to active transcription sites. Mol Biol Cell. 2002;13:2001-15 pubmed
	Höpfl G, Wenger R, Ziegler U, Stallmach T, Gardelle O, Achermann R, et al. Rescue of hypoxia-inducible factor-1alpha-deficient tumor growth by wild-type cells is independent of vascular endothelial growth factor. Cancer Res. 2002;62:2962-70 pubmed
	Powell W, Hahn M. Identification and functional characterization of hypoxia-inducible factor 2alpha from the estuarine teleost, Fundulus heteroclitus: interaction of HIF-2alpha with two ARNT2 splice variants. J Exp Zool. 2002;294:17-29 pubmed publisher
	Marti H, Katschinski D, Wagner K, Schaffer L, Stier B, Wenger R. Isoform-specific expression of hypoxia-inducible factor-1alpha during the late stages of mouse spermiogenesis. Mol Endocrinol. 2002;16:234-43 pubmed
	Komura K, Hayashi S, Makino I, Poellinger L, Tanaka H. Aryl hydrocarbon receptor/dioxin receptor in human monocytes and macrophages. Mol Cell Biochem. 2001;226:107-18 pubmed
	Chun Y, Choi E, Yeo E, Lee J, Kim M, Park J. A new HIF-1 alpha variant induced by zinc ion suppresses HIF-1-mediated hypoxic responses. J Cell Sci. 2001;114:4051-61 pubmed
	Yin H, Blanchard K. DNA methylation represses the expression of the human erythropoietin gene by two different mechanisms. Blood. 2000;95:111-9 pubmed
	Zaman K, Ryu H, Hall D, O Donovan K, Lin K, Miller M, et al. Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1), an. J Neurosci. 1999;19:9821-30 pubmed
	Wenger R, Camenisch G, Desbaillets I, Chilov D, Gassmann M. Up-regulation of hypoxia-inducible factor-1alpha is not sufficient for hypoxic/anoxic p53 induction. Cancer Res. 1998;58:5678-80 pubmed
	Hord N, Perdew G. Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator. Mol Pharmacol. 1994;46:618-26 pubmed

product information

Product Type :

Antibody

Product Name :

HIF-1 beta Monoclonal Antibody (2B10)

Catalog # :

MA1-515

Quantity :

100 uL

Price :

US 451.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 5 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

2B10

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.

Immunogen :

Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.

Format :

Liquid

Applications w/Dilutions :

Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 5 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:3,000

Aliases :

AHA-1; ARNT; ARNT protein; Arnt1; ARNT1a; ARNT1b; aryl hydrocarbon receptor nuclear translocater; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; aryl hydrocarbon receptor nuclear translocator type 1a; aryl hydrocarbon receptor nuclear translocator type 1b; bHLHe2; Class E basic helix-loop-helix protein 2; D3Ertd557e; dioxin receptor, nuclear translocator; Drnt; ESTM42; hif 1; HIF1 beta; HIF-1 beta; Hif1b; HIF1BETA; HIF-1beta; HIF-1-beta; HIF1-beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; hypoxia-inducible factor 1-beta; mKIAA4051; TANGO; W08714; zgc:136664

more info or order :

Invitrogen product webpage