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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
AHR Monoclonal Antibody (RPT9)
catalog :
MA1-513
quantity :
100 uL
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RPT9
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human
Tsuji N, Fukuda K, Nagata Y, Okada H, Haga A, Hatakeyama S, et al. The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90. FEBS Open Bio. 2014;4:796-803 pubmed publisher
  • immunocytochemistry; human; 1:100
Nakamura M, Ueda Y, Hayashi M, Kato H, Furuhashi T, Morita A. Tobacco smoke-induced skin pigmentation is mediated by the aryl hydrocarbon receptor. Exp Dermatol. 2013;22:556-8 pubmed publisher
  • western blot; human
Fukushima K, Tsukimori K, Li D, Takao T, Morokuma S, Kato K, et al. Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines. Hum Exp Toxicol. 2012;31:550-6 pubmed publisher
  • immunoprecipitation; human
Colombo F, Falvella F, Galvan A, Frullanti E, Kunitoh H, Ushijima T, et al. A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A. Mol Cancer. 2011;10:81 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Furumatsu K, Nishiumi S, Kawano Y, Ooi M, Yoshie T, Shiomi Y, et al. A role of the aryl hydrocarbon receptor in attenuation of colitis. Dig Dis Sci. 2011;56:2532-44 pubmed publisher
  • EMSA; rat
Wang X, Hawkins B, Miller D. Aryl hydrocarbon receptor-mediated up-regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier. FASEB J. 2011;25:644-52 pubmed publisher
  • chromatin immunoprecipitation; mouse
Reyes Hernández O, Mejía García A, Sánchez Ocampo E, Cabañas Cortés M, Ramirez P, Chávez González L, et al. Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination. Biochem Pharmacol. 2010;80:932-40 pubmed publisher
  • chromatin immunoprecipitation; human
Tan K, Wang B, Yang M, Boutros P, Macaulay J, Xu H, et al. Aryl hydrocarbon receptor is a transcriptional activator of the human breast cancer resistance protein (BCRP/ABCG2). Mol Pharmacol. 2010;78:175-85 pubmed publisher
  • western blot; human
Iba M, Shin M, Caccavale R. Cytochromes P4501 (CYP1): catalytic activities and inducibility by diesel exhaust particle extract and benzo[a]pyrene in intact human lung ex vivo. Toxicology. 2010;273:35-44 pubmed publisher
  • western blot; human
Watabe Y, Nazuka N, Tezuka M, Shimba S. Aryl hydrocarbon receptor functions as a potent coactivator of E2F1-dependent trascription activity. Biol Pharm Bull. 2010;33:389-97 pubmed
  • western blot; mouse
Nishiumi S, Yoshida M, Azuma T, Yoshida K, Ashida H. 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs an insulin signaling pathway through the induction of tumor necrosis factor-alpha in adipocytes. Toxicol Sci. 2010;115:482-91 pubmed publisher
  • EMSA; human
Marchand A, Tomkiewicz C, Marchandeau J, Boitier E, Barouki R, Garlatti M. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces insulin-like growth factor binding protein-1 gene expression and counteracts the negative effect of insulin. Mol Pharmacol. 2005;67:444-52 pubmed
Chen W, Mi C, Zhang Y, Yang Y, Huang W, Xu Z, et al. Defective Homologous Recombination Repair By Up-Regulating Lnc-HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage. Adv Sci (Weinh). 2024;11:e2207435 pubmed publisher
Heo M, Suh J, Lee S, Poulsen K, An Y, Moorthy B, et al. Aryl hydrocarbon receptor maintains hepatic mitochondrial homeostasis in mice. Mol Metab. 2023;72:101717 pubmed publisher
Wang M, Li L, Qian J, Wang N, Bao J, Lu J, et al. Periodontitis salivary microbiota exacerbates nonalcoholic fatty liver disease in high-fat diet-induced obese mice. iScience. 2023;26:106346 pubmed publisher
Wang Y, Sun J, Zhu K, Wang D, Zhao X, Zhang H, et al. Microglial aryl hydrocarbon receptor enhances phagocytic function via SYK and promotes remyelination in the cuprizone mouse model of demyelination. J Neuroinflammation. 2023;20:83 pubmed publisher
Cano S xe1 nchez J, Murillo Gonz xe1 lez F, de Jes xfa s Aguilar J, Caba xf1 as Cort xe9 s M, Tirado Garibay A, Elizondo G. The Aryl Hydrocarbon Receptor Ligand 6-Formylindolo(3,2-b)carbazole Promotes Estrogen Receptor Alpha and c-Fos Protein Degradation and Inhibits MCF-7 Cell Proliferation and Migration. Pharmacology. 2023;108:157-165 pubmed publisher
Liu L, Xu J, Xu X, Mao T, Niu W, Wu X, et al. Intestinal Stem Cells Damaged by Deoxycholic Acid via AHR Pathway Contributes to Mucosal Barrier Dysfunction in High-Fat Feeding Mice. Int J Mol Sci. 2022;23: pubmed publisher
Sun H, Han W, Wen J, Ma X. IL4I1 and tryptophan metabolites enhance AHR signals to facilitate colorectal cancer progression and immunosuppression. Am J Transl Res. 2022;14:7758-7770 pubmed
Kim Y, Ko B, Kim D, Tak J, Han C, Cho J, et al. Induction of the hepatic aryl hydrocarbon receptor by alcohol dysregulates autophagy and phospholipid metabolism via PPP2R2D. Nat Commun. 2022;13:6080 pubmed publisher
Xu L, Yuan Y, Yu H, Wang S, Wang P. LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis. J Transl Med. 2022;20:350 pubmed publisher
van de Veerdonk F, Renga G, Pariano M, Bellet M, Servillo G, Fallarino F, et al. Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy. J Clin Invest. 2021;: pubmed publisher
Desmet N, Dhusia K, Qi W, Doseff A, Bhattacharya S, Gilad A. Bioengineering of Genetically Encoded Gene Promoter Repressed by the Flavonoid Apigenin for Constructing Intracellular Sensor for Molecular Events. Biosensors (Basel). 2021;11: pubmed publisher
Yang X, Liu H, Ye T, Duan C, Lv P, Wu X, et al. AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization. Theranostics. 2020;10:12011-12025 pubmed publisher
Mohammadi S, Memarian A, Sedighi S, Behnampour N, Yazdani Y. Immunoregulatory effects of indole-3-carbinol on monocyte-derived macrophages in systemic lupus erythematosus: A crucial role for aryl hydrocarbon receptor. Autoimmunity. 2018;51:199-209 pubmed publisher
Wang L, Zhai D, Ruan B, Xu C, Ye Z, Lu H, et al. Quaking Deficiency Amplifies Inflammation in Experimental Endotoxemia via the Aryl Hydrocarbon Receptor/Signal Transducer and Activator of Transcription 1-NF-κB Pathway. Front Immunol. 2017;8:1754 pubmed publisher
Neuschäfer Rube F, Schraplau A, Schewe B, Lieske S, Krützfeldt J, Ringel S, et al. Arylhydrocarbon receptor-dependent mIndy (Slc13a5) induction as possible contributor to benzo[a]pyrene-induced lipid accumulation in hepatocytes. Toxicology. 2015;337:1-9 pubmed publisher
Chen Y, Beischlag T, Kim J, Perdew G, Stallcup M. Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor. J Biol Chem. 2006;281:12242-7 pubmed
Kim J, Stallcup M. Role of the coiled-coil coactivator (CoCoA) in aryl hydrocarbon receptor-mediated transcription. J Biol Chem. 2004;279:49842-8 pubmed
Gouédard C, Barouki R, Morel Y. Dietary polyphenols increase paraoxonase 1 gene expression by an aryl hydrocarbon receptor-dependent mechanism. Mol Cell Biol. 2004;24:5209-22 pubmed
Fukuda I, Nishiumi S, Yabushita Y, Mukai R, Kodoi R, Hashizume K, et al. A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists. J Immunol Methods. 2004;287:187-201 pubmed
Savouret J, Antenos M, Quesne M, Xu J, Milgrom E, Casper R. 7-ketocholesterol is an endogenous modulator for the arylhydrocarbon receptor. J Biol Chem. 2001;276:3054-9 pubmed
Meyer B, Perdew G. Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization. Biochemistry. 1999;38:8907-17 pubmed
product information
Product Type :
Antibody
Product Name :
AHR Monoclonal Antibody (RPT9)
Catalog # :
MA1-513
Quantity :
100 uL
Price :
US 471.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
ChIP Assay: 1:100, Flow Cytometry: 1:50, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
Species :
Human, Mouse, Rat
Clone :
RPT9
Isotype :
IgG3, kappa
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.
Immunogen :
Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 1:100, Flow Cytometry: 1:50, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
Aliases :
Ah; Ah receptor; Ahh; Ahr; Ahre; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; aryl-hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor; In
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA