Glucocorticoid Receptor Monoclonal Antibody (BuGR2) | Invitrogen MA1-510 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Glucocorticoid Receptor Monoclonal Antibody (BuGR2)

catalog :

MA1-510

quantity :

100 µg

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BuGR2

reactivity :

African green monkey, fission yeast, guinea pig, human, mouse, rat, domestic sheep, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 177

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig s1d	Marchetta P, Eckert P, Lukowski R, Ruth P, Singer W, R xfc ttiger L, et al. Loss of central mineralocorticoid or glucocorticoid receptors impacts auditory nerve processing in the cochlea. iScience. 2022;25:103981 pubmed publisher
immunohistochemistry - frozen section; rat; loading ...; fig 4a	Yasuda M, Shinoda M, Honda K, Fujita M, Kawata A, Nagashima H, et al. Maternal Separation Induces Orofacial Mechanical Allodynia in Adulthood. J Dent Res. 2016;95:1191-7 pubmed publisher
western blot; mouse; 1:6000; fig 3	Demuyser T, Bentea E, Deneyer L, Albertini G, Massie A, Smolders I. Disruption of the HPA-axis through corticosterone-release pellets induces robust depressive-like behavior and reduced BDNF levels in mice. Neurosci Lett. 2016;626:119-25 pubmed publisher
chromatin immunoprecipitation; mouse; fig 1d western blot; mouse; fig s3c	Ma X, Xu L, Mueller E. Forkhead box A3 mediates glucocorticoid receptor function in adipose tissue. Proc Natl Acad Sci U S A. 2016;113:3377-82 pubmed publisher
immunohistochemistry - paraffin section; human	Baker G, Murphy T, Block T, Nguyen D, Lynch F. Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancer. Cancer Manag Res. 2015;7:361-8 pubmed publisher
chromatin immunoprecipitation; rat; fig 2	Arango Lievano M, Lambert W, Bath K, Garabedian M, Chao M, Jeanneteau F. Neurotrophic-priming of glucocorticoid receptor signaling is essential for neuronal plasticity to stress and antidepressant treatment. Proc Natl Acad Sci U S A. 2015;112:15737-42 pubmed publisher
chromatin immunoprecipitation; mouse	Stavreva D, Coulon A, Baek S, Sung M, John S, Stixova L, et al. Dynamics of chromatin accessibility and long-range interactions in response to glucocorticoid pulsing. Genome Res. 2015;25:845-57 pubmed publisher
immunohistochemistry; domestic sheep; 1:50 western blot; domestic sheep; 1:1000	Shang H, Meng W, Sloboda D, Li S, Ehrlich L, Plagemann A, et al. Effects of maternal dexamethasone treatment early in pregnancy on glucocorticoid receptors in the ovine placenta. Reprod Sci. 2015;22:534-44 pubmed publisher
western blot; rat; 1:1000	Connors E, Shaik A, Migliore M, Kentner A. Environmental enrichment mitigates the sex-specific effects of gestational inflammation on social engagement and the hypothalamic pituitary adrenal axis-feedback system. Brain Behav Immun. 2014;42:178-90 pubmed publisher
western blot; rat	Zhu R, Lu X, Pradhan M, Armstrong S, Storchan G, Chow C, et al. A kinase-independent activity of Cdk9 modulates glucocorticoid receptor-mediated gene induction. Biochemistry. 2014;53:1753-67 pubmed publisher
immunohistochemistry; mouse	Sultana R, Theodoraki M, Caplan A. Specificity in the actions of the UBR1 ubiquitin ligase in the degradation of nuclear receptors. FEBS Open Bio. 2013;3:394-7 pubmed publisher
immunoprecipitation; African green monkey	Luo M, Lu X, Zhu R, Zhang Z, Chow C, Li R, et al. A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties. J Biol Chem. 2013;288:34055-72 pubmed publisher
chromatin immunoprecipitation; rat; 3 ug/time	Evans A, Liu Y, MACGREGOR R, Huang V, Aguilera G. Regulation of hypothalamic corticotropin-releasing hormone transcription by elevated glucocorticoids. Mol Endocrinol. 2013;27:1796-807 pubmed publisher
western blot; rat	Adzic M, Lukic I, Mitic M, Djordjevic J, Elaković I, Djordjevic A, et al. Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: effects on energy metabolism. Psychoneuroendocrinology. 2013;38:2914-24 pubmed publisher
chromatin immunoprecipitation; human chromatin immunoprecipitation; rat	Lambert W, Xu C, Neubert T, Chao M, Garabedian M, Jeanneteau F. Brain-derived neurotrophic factor signaling rewrites the glucocorticoid transcriptome via glucocorticoid receptor phosphorylation. Mol Cell Biol. 2013;33:3700-14 pubmed publisher
chromatin immunoprecipitation; mouse	Zhang L, Prak L, Rayon Estrada V, Thiru P, Flygare J, Lim B, et al. ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors. Nature. 2013;499:92-6 pubmed publisher
immunohistochemistry; rat; 1:1000	Wisłowska Stanek A, Lehner M, Skorzewska A, Maciejak P, Szyndler J, Turzynska D, et al. Corticosterone modulates fear responses and the expression of glucocorticoid receptors in the brain of high-anxiety rats. Neurosci Lett. 2013;533:17-22 pubmed publisher
chromatin immunoprecipitation; rat; 2 ug/time	Zhang Z, Sun Y, Cho Y, Chow C, Simons S. PA1 protein, a new competitive decelerator acting at more than one step to impede glucocorticoid receptor-mediated transactivation. J Biol Chem. 2013;288:42-58 pubmed publisher
western blot; rat western blot; human; fig 1d	Blackford J, Guo C, Zhu R, Dougherty E, Chow C, Simons S. Identification of location and kinetically defined mechanism of cofactors and reporter genes in the cascade of steroid-regulated transactivation. J Biol Chem. 2012;287:40982-95 pubmed publisher
western blot; rat; 1:6000	Cory Slechta D, Virgolini M, Liu S, Weston D. Enhanced stimulus sequence-dependent repeated learning in male offspring after prenatal stress alone or in conjunction with lead exposure. Neurotoxicology. 2012;33:1188-202 pubmed publisher
chromatin immunoprecipitation; human	Awasthi S, Simons S. Separate regions of glucocorticoid receptor, coactivator TIF2, and comodulator STAMP modify different parameters of glucocorticoid-mediated gene induction. Mol Cell Endocrinol. 2012;355:121-34 pubmed publisher
immunoprecipitation; mouse	Pang X, Cheng J, Kim J, Matsubara T, Krausz K, Gonzalez F. Expression and regulation of human fetal-specific CYP3A7 in mice. Endocrinology. 2012;153:1453-63 pubmed publisher
immunoprecipitation; rat	Jeanneteau F, Lambert W, Ismaili N, Bath K, Lee F, Garabedian M, et al. BDNF and glucocorticoids regulate corticotrophin-releasing hormone (CRH) homeostasis in the hypothalamus. Proc Natl Acad Sci U S A. 2012;109:1305-10 pubmed publisher
immunoprecipitation; mouse	Voss T, Schiltz R, Sung M, Yen P, Stamatoyannopoulos J, Biddie S, et al. Dynamic exchange at regulatory elements during chromatin remodeling underlies assisted loading mechanism. Cell. 2011;146:544-54 pubmed publisher
western blot; mouse immunoprecipitation; rat western blot; rat; 0.2 ug/ml	Gallo L, Lagadari M, Piwien Pilipuk G, Galigniana M. The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress. J Biol Chem. 2011;286:30152-60 pubmed publisher
chromatin immunoprecipitation; mouse	Wiench M, John S, Baek S, Johnson T, Sung M, Escobar T, et al. DNA methylation status predicts cell type-specific enhancer activity. EMBO J. 2011;30:3028-39 pubmed publisher
chromatin immunoprecipitation; mouse	Siersbæk R, Nielsen R, John S, Sung M, Baek S, Loft A, et al. Extensive chromatin remodelling and establishment of transcription factor 'hotspots' during early adipogenesis. EMBO J. 2011;30:1459-72 pubmed publisher
flow cytometry; human	Gao L, Wang J, Xiang M, Fan Y, Zhang Z, Wang K. Expression of human glucocorticoid receptor in T lymphocytes in acute-on-chronic hepatitis B liver failure. Dig Dis Sci. 2011;56:2605-12 pubmed publisher
immunoprecipitation; human	John S, Sabo P, Thurman R, Sung M, Biddie S, Johnson T, et al. Chromatin accessibility pre-determines glucocorticoid receptor binding patterns. Nat Genet. 2011;43:264-8 pubmed publisher
immunohistochemistry; rat; 1:100 western blot; rat; 1:200	Prager E, Brielmaier J, Bergstrom H, McGuire J, Johnson L. Localization of mineralocorticoid receptors at mammalian synapses. PLoS ONE. 2010;5:e14344 pubmed publisher
western blot; mouse	Bertucci P, Quaglino A, Pozzi A, Kordon E, Pecci A. Glucocorticoid-induced impairment of mammary gland involution is associated with STAT5 and STAT3 signaling modulation. Endocrinology. 2010;151:5730-40 pubmed publisher
western blot; human	Rossi George A, Virgolini M, Weston D, Thiruchelvam M, Cory Slechta D. Interactions of lifetime lead exposure and stress: behavioral, neurochemical and HPA axis effects. Neurotoxicology. 2011;32:83-99 pubmed publisher
western blot; mouse	Quintá H, Maschi D, Gomez Sanchez C, Piwien Pilipuk G, Galigniana M. Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth. J Neurochem. 2010;115:716-34 pubmed publisher
western blot; mouse; 0.25 ug/ml	Wang A, Morishima Y, Clapp K, Peng H, Pratt W, Gestwicki J, et al. Inhibition of hsp70 by methylene blue affects signaling protein function and ubiquitination and modulates polyglutamine protein degradation. J Biol Chem. 2010;285:15714-23 pubmed publisher
immunoprecipitation; human	Mandal A, Gibney P, Nillegoda N, Theodoraki M, Caplan A, Morano K. Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system. Mol Biol Cell. 2010;21:1439-48 pubmed publisher
immunoprecipitation; rat	Arambasić J, Poznanovic G, Ivanovic Matic S, Bogojevic D, Mihailovic M, Uskoković A, et al. Association of the glucocorticoid receptor with STAT3, C/EBPbeta, and the hormone-responsive element within the rat haptoglobin gene promoter during the acute phase response. IUBMB Life. 2010;62:227-36 pubmed publisher
immunoprecipitation; rat western blot; rat	Elaković I, Vasiljević D, Adzic M, Djordjevic A, Djordjevic J, Radojcic M, et al. Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine. Eur J Pharmacol. 2010;632:79-85 pubmed publisher
western blot; mouse	Galigniana M, Erlejman A, Monte M, Gomez Sanchez C, Piwien Pilipuk G. The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events. Mol Cell Biol. 2010;30:1285-98 pubmed publisher
western blot; rat	Adzic M, Djordjevic A, Demonacos C, Krstic Demonacos M, Radojcic M. The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats. Int J Biochem Cell Biol. 2009;41:2181-8 pubmed publisher
immunohistochemistry; fission yeast; 5 ug/ml	Simmons R, Satterfield M, Welsh T, Bazer F, Spencer T. HSD11B1, HSD11B2, PTGS2, and NR3C1 expression in the peri-implantation ovine uterus: effects of pregnancy, progesterone, and interferon tau. Biol Reprod. 2010;82:35-43 pubmed publisher
immunoprecipitation; human immunocytochemistry; human	Echeverria P, Mazaira G, Erlejman A, Gomez Sanchez C, Piwien Pilipuk G, Galigniana M. Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin beta. Mol Cell Biol. 2009;29:4788-97 pubmed publisher
western blot; rat	Kawabata K, Kawai Y, Terao J. Suppressive effect of quercetin on acute stress-induced hypothalamic-pituitary-adrenal axis response in Wistar rats. J Nutr Biochem. 2010;21:374-80 pubmed publisher
immunohistochemistry; mouse	Dong H, Yuede C, Yoo H, Martin M, Deal C, Mace A, et al. Corticosterone and related receptor expression are associated with increased beta-amyloid plaques in isolated Tg2576 mice. Neuroscience. 2008;155:154-63 pubmed publisher
immunocytochemistry; African green monkey; 1:350	Haffner M, Jurgeit A, Berlato C, Geley S, Parajuli N, Yoshimura A, et al. Interaction and functional interference of glucocorticoid receptor and SOCS1. J Biol Chem. 2008;283:22089-96 pubmed publisher
EMSA; rat immunoprecipitation; rat western blot; rat; 1:5000	Vujcić M, Velickovic N, Ruzdijic S. Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. Comp Biochem Physiol B Biochem Mol Biol. 2007;148:463-9 pubmed
western blot; rat	Wang D, Wang Q, Awasthi S, Simons S. Amino-terminal domain of TIF2 is involved in competing for corepressor binding to glucocorticoid and progesterone receptors. Biochemistry. 2007;46:8036-49 pubmed
immunohistochemistry; domestic sheep; 1:60	Antonow Schlorke I, Muller T, Brodhun M, Wicher C, Schubert H, Nathanielsz P, et al. Betamethasone-related acute alterations of microtubule-associated proteins in the fetal sheep brain are reversible and independent of age during the last one-third of gestation. Am J Obstet Gynecol. 2007;196:553.e1-6 pubmed
western blot; rat; 1:250	Morsink M, Joels M, Sarabdjitsingh R, Meijer O, de Kloet E, Datson N. The dynamic pattern of glucocorticoid receptor-mediated transcriptional responses in neuronal PC12 cells. J Neurochem. 2006;99:1282-98 pubmed
immunoprecipitation; mouse immunocytochemistry; mouse; 1:50 western blot; mouse; 1:500	Presman D, Hoijman E, Ceballos N, Galigniana M, Pecci A. Melatonin inhibits glucocorticoid receptor nuclear translocation in mouse thymocytes. Endocrinology. 2006;147:5452-9 pubmed
flow cytometry; mouse	Erlacher M, Knoflach M, Stec I, Böck G, Wick G, Wiegers G. TCR signaling inhibits glucocorticoid-induced apoptosis in murine thymocytes depending on the stage of development. Eur J Immunol. 2005;35:3287-96 pubmed
western blot; rat; 1:6000	Virgolini M, Chen K, Weston D, Bauter M, Cory Slechta D. Interactions of chronic lead exposure and intermittent stress: consequences for brain catecholamine systems and associated behaviors and HPA axis function. Toxicol Sci. 2005;87:469-82 pubmed
immunohistochemistry; mouse	Webster J, Sternberg E. Anthrax lethal toxin represses glucocorticoid receptor (GR) transactivation by inhibiting GR-DNA binding in vivo. Mol Cell Endocrinol. 2005;241:21-31 pubmed
	Qiao X, Yang Y, Zhao Y, Wu X, Zhang L, Cai X, et al. Aurora Kinase A Regulates Cell Transitions in Glucocorticoid-Induced Bone Loss. Cells. 2023;12: pubmed publisher
	Toh P, Seale L, Berry M, Torres D. Prolonged maternal exposure to glucocorticoids alters selenoprotein expression in the developing brain. Front Mol Neurosci. 2023;16:1115993 pubmed publisher
	Yao D, Lu Y, Li L, Wang S, Mu Y, Ding C, et al. Prolactin and glucocorticoid receptors in the prefrontal cortex are associated with anxiety-like behavior in prenatally stressed adolescent offspring rats. J Neuroendocrinol. 2023;35:e13231 pubmed publisher
	Præstholm S, Correia C, Goitea V, Siersbæk M, Jørgensen M, Havelund J, et al. Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage. Cell Rep. 2021;37:109938 pubmed publisher
	Kang J, Kim D, Lee J, Hwang S, Kim G, Hyun S, et al. Korean Red Ginseng Ameliorates Fatigue via Modulation of 5-HT and Corticosterone in a Sleep-Deprived Mouse Model. Nutrients. 2021;13: pubmed publisher
	Yao J, Leng L, Fu W, Li J, Bronner C, Bucala R. ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus. Arthritis Rheumatol. 2021;73:1931-1942 pubmed publisher
	Johnson T, Paakinaho V, Kim S, Hager G, Presman D. Genome-wide binding potential and regulatory activity of the glucocorticoid receptor's monomeric and dimeric forms. Nat Commun. 2021;12:1987 pubmed publisher
	Yu P, Liu M, Zhang B, Yu Y, Su E, Xie S, et al. Cardiomyocyte-restricted high-mobility group box 1 (HMGB1) deletion leads to small heart and glycolipid metabolic disorder through GR/PGC-1α signalling. Cell Death Discov. 2020;6:106 pubmed publisher
	Adrienne McGinn M, Edwards K, Edwards S. Chronic inflammatory pain alters alcohol-regulated frontocortical signaling and associations between alcohol drinking and thermal sensitivity. Neurobiol Pain. 2020;8:100052 pubmed publisher
	Mazaira G, Echeverria P, Galigniana M. Nucleocytoplasmic shuttling of the glucocorticoid receptor is influenced by tetratricopeptide repeat-containing proteins. J Cell Sci. 2020;133: pubmed publisher
	Palumbo M, Dominguez S, Dong H. Sex differences in hypothalamic-pituitary-adrenal axis regulation after chronic unpredictable stress. Brain Behav. 2020;10:e01586 pubmed publisher
	Ogara M, Rodríguez Seguí S, Marini M, Nacht A, Stortz M, Levi V, et al. The glucocorticoid receptor interferes with progesterone receptor-dependent genomic regulation in breast cancer cells. Nucleic Acids Res. 2019;47:10645-10661 pubmed publisher
	Stavreva D, Garcia D, Fettweis G, Gudla P, Zaki G, Soni V, et al. Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility. Mol Cell. 2019;75:1161-1177.e11 pubmed publisher
	Kalvisa A, Siersbæk M, Præstholm S, Christensen L, Nielsen R, Stöhr O, et al. Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver. PLoS Biol. 2018;16:e2006249 pubmed publisher
	Sobolewski M, Varma G, Adams B, Anderson D, Schneider J, Cory Slechta D. Developmental Lead Exposure and Prenatal Stress Result in Sex-Specific Reprograming of Adult Stress Physiology and Epigenetic Profiles in Brain. Toxicol Sci. 2018;163:478-489 pubmed publisher
	Jubb A, Boyle S, Hume D, Bickmore W. Glucocorticoid Receptor Binding Induces Rapid and Prolonged Large-Scale Chromatin Decompaction at Multiple Target Loci. Cell Rep. 2017;21:3022-3031 pubmed publisher
	Oh K, Patel H, Gottschalk R, Lee W, Baek S, Fraser I, et al. Anti-Inflammatory Chromatinscape Suggests Alternative Mechanisms of Glucocorticoid Receptor Action. Immunity. 2017;47:298-309.e5 pubmed publisher
	Gauthier M, Chakraborty K, Oriss T, Raundhal M, Das S, Chen J, et al. Severe asthma in humans and mouse model suggests a CXCL10 signature underlies corticosteroid-resistant Th1 bias. JCI Insight. 2017;2: pubmed publisher
	Flom G, Lemieszek M, Fortunato E, Johnson J. Farnesylation of Ydj1 is required for in vivo interaction with Hsp90 client proteins. Mol Biol Cell. 2008;19:5249-58 pubmed publisher
	Hawle P, Siepmann M, Harst A, Siderius M, Reusch H, Obermann W. The middle domain of Hsp90 acts as a discriminator between different types of client proteins. Mol Cell Biol. 2006;26:8385-95 pubmed
	Heitzer M, DeFranco D. Mechanism of action of Hic-5/androgen receptor activator 55, a LIM domain-containing nuclear receptor coactivator. Mol Endocrinol. 2006;20:56-64 pubmed
	Barish G, Downes M, Alaynick W, Yu R, Ocampo C, Bookout A, et al. A Nuclear Receptor Atlas: macrophage activation. Mol Endocrinol. 2005;19:2466-77 pubmed
	Carrigan P, Riggs D, Chinkers M, Smith D. Functional comparison of human and Drosophila Hop reveals novel role in steroid receptor maturation. J Biol Chem. 2005;280:8906-11 pubmed
	Brkljacic J, Milutinović D, Dundjerski J, Matić G. Mercury stimulates rat liver glucocorticoid receptor association with Hsp90 and Hsp70. J Biochem Mol Toxicol. 2004;18:257-60 pubmed
	Cho S, Kagan B, Blackford J, Szapary D, Simons S. Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and Ubc9. Mol Endocrinol. 2005;19:290-311 pubmed
	Harrell J, Murphy P, Morishima Y, Chen H, Mansfield J, Galigniana M, et al. Evidence for glucocorticoid receptor transport on microtubules by dynein. J Biol Chem. 2004;279:54647-54 pubmed
	Wei Q, Lu X, Liu L, Schafer G, Shieh K, Burke S, et al. Glucocorticoid receptor overexpression in forebrain: a mouse model of increased emotional lability. Proc Natl Acad Sci U S A. 2004;101:11851-6 pubmed
	Billecke S, Draganov D, Morishima Y, Murphy P, Dunbar A, Pratt W, et al. The role of hsp90 in heme-dependent activation of apo-neuronal nitric-oxide synthase. J Biol Chem. 2004;279:30252-8 pubmed
	Hoetzenecker W, Meingassner J, Ecker R, Stingl G, Stuetz A, Elbe Bürger A. Corticosteroids but not pimecrolimus affect viability, maturation and immune function of murine epidermal Langerhans cells. J Invest Dermatol. 2004;122:673-84 pubmed
	Nelson G, Prapapanich V, Carrigan P, Roberts P, Riggs D, Smith D. The heat shock protein 70 cochaperone hip enhances functional maturation of glucocorticoid receptor. Mol Endocrinol. 2004;18:1620-30 pubmed
	Wang Q, Blackford J, Song L, Huang Y, Cho S, Simons S. Equilibrium interactions of corepressors and coactivators with agonist and antagonist complexes of glucocorticoid receptors. Mol Endocrinol. 2004;18:1376-95 pubmed
	Kutzler M, Ruane E, Coksaygan T, Vincent S, Nathanielsz P. Effects of three courses of maternally administered dexamethasone at 0.7, 0.75, and 0.8 of gestation on prenatal and postnatal growth in sheep. Pediatrics. 2004;113:313-9 pubmed
	Viegas L, Vicent G, Barañao J, Beato M, Pecci A. Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4. J Biol Chem. 2004;279:9831-9 pubmed
	Morishima Y, Kanelakis K, Murphy P, Lowe E, Jenkins G, Osawa Y, et al. The hsp90 cochaperone p23 is the limiting component of the multiprotein hsp90/hsp70-based chaperone system in vivo where it acts to stabilize the client protein: hsp90 complex. J Biol Chem. 2003;278:48754-63 pubmed
	Hoijman E, Rocha Viegas L, Keller Sarmiento M, Rosenstein R, Pecci A. Involvement of Bax protein in the prevention of glucocorticoid-induced thymocytes apoptosis by melatonin. Endocrinology. 2004;145:418-25 pubmed
	Kasai M, Guerrero Santoro J, Friedman R, Leman E, Getzenberg R, DeFranco D. The Group 3 LIM domain protein paxillin potentiates androgen receptor transactivation in prostate cancer cell lines. Cancer Res. 2003;63:4927-35 pubmed
	Tjandra K, Le T, Swain M. Glucocorticoid receptors are downregulated in hepatic T lymphocytes in rats with experimental cholangitis. Gut. 2003;52:1363-70 pubmed
	Walther R, Lamprecht C, Ridsdale A, Groulx I, Lee S, Lefebvre Y, et al. Nuclear export of the glucocorticoid receptor is accelerated by cell fusion-dependent release of calreticulin. J Biol Chem. 2003;278:37858-64 pubmed
	Murphy P, Morishima Y, Chen H, Galigniana M, Mansfield J, Simons S, et al. Visualization and mechanism of assembly of a glucocorticoid receptor.Hsp70 complex that is primed for subsequent Hsp90-dependent opening of the steroid binding cleft. J Biol Chem. 2003;278:34764-73 pubmed
	Lambert J, Nordeen S. CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins. Mol Endocrinol. 2003;17:1085-94 pubmed
	Patel A, Bulloch K. Type II glucocorticoid receptor immunoreactivity in the mossy cells of the rat and the mouse hippocampus. Hippocampus. 2003;13:59-66 pubmed
	Riggs D, Roberts P, Chirillo S, Cheung Flynn J, Prapapanich V, Ratajczak T, et al. The Hsp90-binding peptidylprolyl isomerase FKBP52 potentiates glucocorticoid signaling in vivo. EMBO J. 2003;22:1158-67 pubmed
	Li Q, Su A, Chen J, Lefebvre Y, Haché R. Attenuation of glucocorticoid signaling through targeted degradation of p300 via the 26S proteasome pathway. Mol Endocrinol. 2002;16:2819-27 pubmed
	Brewer J, Kanagawa O, Sleckman B, Muglia L. Thymocyte apoptosis induced by T cell activation is mediated by glucocorticoids in vivo. J Immunol. 2002;169:1837-43 pubmed
	Kanelakis K, Shewach D, Pratt W. Nucleotide binding states of hsp70 and hsp90 during sequential steps in the process of glucocorticoid receptor.hsp90 heterocomplex assembly. J Biol Chem. 2002;277:33698-703 pubmed
	Harrell J, Kurek I, Breiman A, Radanyi C, Renoir J, Pratt W, et al. All of the protein interactions that link steroid receptor.hsp90.immunophilin heterocomplexes to cytoplasmic dynein are common to plant and animal cells. Biochemistry. 2002;41:5581-7 pubmed
	Piwien Pilipuk G, Kanelakis K, Ghini A, Lantos C, Litwack G, Burton G, et al. Modification of an essential amino group in the mineralocorticoid receptor evidences a differential conformational change of the receptor protein upon binding of antagonists, natural agonists and the synthetic agonist 11,19-oxidoprogesterone. Biochim Biophys Acta. 2002;1589:31-48 pubmed
	Murphy E, Spencer R, Sipe K, Herman J. Decrements in nuclear glucocorticoid receptor (GR) protein levels and DNA binding in aged rat hippocampus. Endocrinology. 2002;143:1362-70 pubmed
	Davies T, Ning Y, SANCHEZ E. A new first step in activation of steroid receptors: hormone-induced switching of FKBP51 and FKBP52 immunophilins. J Biol Chem. 2002;277:4597-600 pubmed
	Proulx K, Clavel S, Nault G, Richard D, Walker C. High neonatal leptin exposure enhances brain GR expression and feedback efficacy on the adrenocortical axis of developing rats. Endocrinology. 2001;142:4607-16 pubmed
	Mukhopadhyay S, Wyszomierski S, Gronostajski R, Rosen J. Differential interactions of specific nuclear factor I isoforms with the glucocorticoid receptor and STAT5 in the cooperative regulation of WAP gene transcription. Mol Cell Biol. 2001;21:6859-69 pubmed
	Soubeyrand S, Torrance H, Giffin W, Gong W, Schild Poulter C, Hache R. Activation and autoregulation of DNA-PK from structured single-stranded DNA and coding end hairpins. Proc Natl Acad Sci U S A. 2001;98:9605-10 pubmed
	Biola A, Lefebvre P, Perrin Wolff M, Sturm M, Bertoglio J, Pallardy M. Interleukin-2 inhibits glucocorticoid receptor transcriptional activity through a mechanism involving STAT5 (signal transducer and activator of transcription 5) but not AP-1. Mol Endocrinol. 2001;15:1062-76 pubmed
	Murphy P, Kanelakis K, Galigniana M, Morishima Y, Pratt W. Stoichiometry, abundance, and functional significance of the hsp90/hsp70-based multiprotein chaperone machinery in reticulocyte lysate. J Biol Chem. 2001;276:30092-8 pubmed
	Belikov S, Gelius B, Wrange O. Hormone-induced nucleosome positioning in the MMTV promoter is reversible. EMBO J. 2001;20:2802-11 pubmed
	Mullick J, Anandatheerthavarada H, Amuthan G, Bhagwat S, Biswas G, Camasamudram V, et al. Physical interaction and functional synergy between glucocorticoid receptor and Ets2 proteins for transcription activation of the rat cytochrome P-450c27 promoter. J Biol Chem. 2001;276:18007-17 pubmed
	Morin B, Woodcock G, Nichols L, Holland L. Heterodimerization between the glucocorticoid receptor and the unrelated DNA-binding protein, Xenopus glucocorticoid receptor accessory factor. Mol Endocrinol. 2001;15:458-66 pubmed
	Wyszomierski S, Rosen J. Cooperative effects of STAT5 (signal transducer and activator of transcription 5) and C/EBPbeta (CCAAT/enhancer-binding protein-beta) on beta-casein gene transcription are mediated by the glucocorticoid receptor. Mol Endocrinol. 2001;15:228-40 pubmed
	Savory J, Préfontaine G, Lamprecht C, Liao M, Walther R, Lefebvre Y, et al. Glucocorticoid receptor homodimers and glucocorticoid-mineralocorticoid receptor heterodimers form in the cytoplasm through alternative dimerization interfaces. Mol Cell Biol. 2001;21:781-93 pubmed
	Kanelakis K, Murphy P, Galigniana M, Morishima Y, Takayama S, Reed J, et al. hsp70 interacting protein Hip does not affect glucocorticoid receptor folding by the hsp90-based chaperone machinery except to oppose the effect of BAG-1. Biochemistry. 2000;39:14314-21 pubmed
	Rajapandi T, Greene L, Eisenberg E. The molecular chaperones Hsp90 and Hsc70 are both necessary and sufficient to activate hormone binding by glucocorticoid receptor. J Biol Chem. 2000;275:22597-604 pubmed
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	Giannoukos G, Silverstein A, Pratt W, Simons S. The seven amino acids (547-553) of rat glucocorticoid receptor required for steroid and hsp90 binding contain a functionally independent LXXLL motif that is critical for steroid binding. J Biol Chem. 1999;274:36527-36 pubmed
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	Nogami H, Inoue K, Moriya H, Ishida A, Kobayashi S, Hisano S, et al. Regulation of growth hormone-releasing hormone receptor messenger ribonucleic acid expression by glucocorticoids in MtT-S cells and in the pituitary gland of fetal rats. Endocrinology. 1999;140:2763-70 pubmed
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	Wyszomierski S, Yeh J, Rosen J. Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation. Mol Endocrinol. 1999;13:330-43 pubmed
	Jun S, Chen Z, Pace M, Shaul P. Glucocorticoids downregulate cyclooxygenase-1 gene expression and prostacyclin synthesis in fetal pulmonary artery endothelium. Circ Res. 1999;84:193-200 pubmed
	Savory J, Hsu B, Laquian I, Giffin W, Reich T, Hache R, et al. Discrimination between NL1- and NL2-mediated nuclear localization of the glucocorticoid receptor. Mol Cell Biol. 1999;19:1025-37 pubmed
	Chandran U, Warren B, Baumann C, Hager G, DeFranco D. The glucocorticoid receptor is tethered to DNA-bound Oct-1 at the mouse gonadotropin-releasing hormone distal negative glucocorticoid response element. J Biol Chem. 1999;274:2372-8 pubmed
	Bender A, Silverstein A, Demady D, Kanelakis K, Noguchi S, Pratt W, et al. Neuronal nitric-oxide synthase is regulated by the Hsp90-based chaperone system in vivo. J Biol Chem. 1999;274:1472-8 pubmed
	Hache R, Tse R, Reich T, Savory J, Lefebvre Y. Nucleocytoplasmic trafficking of steroid-free glucocorticoid receptor. J Biol Chem. 1999;274:1432-9 pubmed
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	Caamaño C, Morano M, Dalman F, Pratt W, Akil H. A conserved proline in the hsp90 binding region of the glucocorticoid receptor is required for hsp90 heterocomplex stabilization and receptor signaling. J Biol Chem. 1998;273:20473-80 pubmed
	Dittmar K, Banach M, Galigniana M, Pratt W. The role of DnaJ-like proteins in glucocorticoid receptor.hsp90 heterocomplex assembly by the reconstituted hsp90.p60.hsp70 foldosome complex. J Biol Chem. 1998;273:7358-66 pubmed
	Barent R, Nair S, Carr D, Ruan Y, Rimerman R, Fulton J, et al. Analysis of FKBP51/FKBP52 chimeras and mutants for Hsp90 binding and association with progesterone receptor complexes. Mol Endocrinol. 1998;12:342-54 pubmed
	Dittmar K, Demady D, Stancato L, Krishna P, Pratt W. Folding of the glucocorticoid receptor by the heat shock protein (hsp) 90-based chaperone machinery. The role of p23 is to stabilize receptor.hsp90 heterocomplexes formed by hsp90.p60.hsp70. J Biol Chem. 1997;272:21213-20 pubmed
	Silverstein A, Galigniana M, Chen M, Owens Grillo J, Chinkers M, Pratt W. Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin. J Biol Chem. 1997;272:16224-30 pubmed
	Dittmar K, Pratt W. Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation. J Biol Chem. 1997;272:13047-54 pubmed
	Sackey F, Hache R, Reich T, Kwast Welfeld J, Lefebvre Y. Determinants of subcellular distribution of the glucocorticoid receptor. Mol Endocrinol. 1996;10:1191-205 pubmed
	Hong H, Kohli K, Garabedian M, Stallcup M. GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors. Mol Cell Biol. 1997;17:2735-44 pubmed
	Chang H, Nathan D, Lindquist S. In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Mol Cell Biol. 1997;17:318-25 pubmed
	Chen M, Silverstein A, Pratt W, Chinkers M. The tetratricopeptide repeat domain of protein phosphatase 5 mediates binding to glucocorticoid receptor heterocomplexes and acts as a dominant negative mutant. J Biol Chem. 1996;271:32315-20 pubmed
	Dittmar K, Hutchison K, Owens Grillo J, Pratt W. Reconstitution of the steroid receptor.hsp90 heterocomplex assembly system of rabbit reticulocyte lysate. J Biol Chem. 1996;271:12833-9 pubmed
	Curtis L, Rarey K. Effect of stress on cochlear glucocorticoid protein. II. Restraint. Hear Res. 1995;92:120-5 pubmed
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	Stancato L, Silverstein A, Gitler C, Groner B, Pratt W. Use of the thiol-specific derivatizing agent N-iodoacetyl-3-[125I]iodotyrosine to demonstrate conformational differences between the unbound and hsp90-bound glucocorticoid receptor hormone binding domain. J Biol Chem. 1996;271:8831-6 pubmed
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	Stancato L, Chow Y, Hutchison K, Perdew G, Jove R, Pratt W. Raf exists in a native heterocomplex with hsp90 and p50 that can be reconstituted in a cell-free system. J Biol Chem. 1993;268:21711-6 pubmed
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	Smets L, van Den Berg J, Acton D, Top B, Van Rooij H, Verwijs Janssen M. BCL-2 expression and mitochondrial activity in leukemic cells with different sensitivity to glucocorticoid-induced apoptosis. Blood. 1994;84:1613-9 pubmed
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image

image 1 :

Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) (Product# MA1-510 ) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor (Product# MA1-510 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) (Product# MA1-510 ) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor (Product# MA1-510 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 3 :

product information

Product Type :

Antibody

Product Name :

Glucocorticoid Receptor Monoclonal Antibody (BuGR2)

Catalog # :

MA1-510

Quantity :

100 µg

Price :

US 446.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Guinea Pig, Human, Mouse, Ovine, Rabbit, Rat, Yeast

Applications :

Flow Cytometry: 1-2 µg/test, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 5 µg/mL, Immunohistochemistry (PFA fixed): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 5 µg/mL

Species :

Guinea Pig, Human, Mouse, Ovine, Rabbit, Rat, Yeast

Clone :

BuGR2

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

Immunogen :

Partially purified rat GR.

Format :

Lyophilized

Applications w/Dilutions :

Aliases :

cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038

more info or order :

Invitrogen product webpage