This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
6x-His Tag Monoclonal Antibody (13/45/31-2)
catalog :
MA1-4806
quantity :
200 µg
price :
US 530
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13/45/31-2
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
product information
Product Type :
Antibody
Product Name :
6x-His Tag Monoclonal Antibody (13/45/31-2)
Catalog # :
MA1-4806
Quantity :
200 µg
Price :
US 530
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Tag
Applications :
ELISA: 0.1-0.4 µg/mL, Flow Cytometry: 5-10 µL/1x10^6 cells, Immunocytochemistry: 1-4 µg/mL, Immunofluorescence: 1-4 µg/mL, Immunohistochemistry (Frozen): 1-4 µg/mL, Immunoprecipitation: 4 µg/mL, Western Blot: 1-4 µg/mL
Species :
Tag
Clone :
13/45/31-2
Isotype :
IgG1, kappa
Storage :
4° C, do not freeze
Description :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Immunogen :
Recombinant (His) 6-p53 protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-0.4 µg/mL, Flow Cytometry: 5-10 µL/1x10^6 cells, Immunocytochemistry: 1-4 µg/mL, Immunofluorescence: 1-4 µg/mL, Immunohistochemistry (Frozen): 1-4 µg/mL, Immunoprecipitation: 4 µg/mL, Western Blot: 1-4 µg/mL
Aliases :
6His tag; 6X His; His Tag; Histidine Tag
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA