Diphtheria Toxin A Monoclonal Antibody (8A4) | Invitrogen MA1-46398 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Diphtheria Toxin A Monoclonal Antibody (8A4)

catalog :

MA1-46398

quantity :

100 µg

price :

US 395.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8A4

product information

Product Type :

Antibody

Product Name :

Diphtheria Toxin A Monoclonal Antibody (8A4)

Catalog # :

MA1-46398

Quantity :

100 µg

Price :

US 395.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bacteria

Applications :

ELISA: 1:100-1:2,000

Species :

Bacteria

Clone :

8A4

Isotype :

IgG2a

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.

Immunogen :

Full length Diphtheria toxin A-subunit protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:100-1:2,000

Aliases :

DT; DTA