Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8) | Invitrogen MA1-46093 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

catalog :

MA1-46093

quantity :

100 µg

price :

US 466

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4H8

reactivity :

Saccharomyces cerevisiae S288C, human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human	Yang Z, Broz D, Noderer W, Ferreira J, Overton K, Spencer S, et al. p53 suppresses muscle differentiation at the myogenin step in response to genotoxic stress. Cell Death Differ. 2015;22:560-73 pubmed publisher
chromatin immunoprecipitation; human; 1:500	Li Q, Pène V, Krishnamurthy S, Cha H, Liang T. Hepatitis C virus infection activates an innate pathway involving IKK-α in lipogenesis and viral assembly. Nat Med. 2013;19:722-9 pubmed publisher
western blot; human	Zhu Q, Wani G, Sharma N, Wani A. Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair. DNA Repair (Amst). 2012;11:942-50 pubmed publisher
	Monteagudo S, Cornelis F, Wang X, De Roover A, Peeters T, Quintiens J, et al. ANP32A represses Wnt signaling across tissues thereby protecting against osteoarthritis and heart disease. Osteoarthritis Cartilage. 2022;30:724-734 pubmed publisher
	Shim H, Horner J, Wu C, Li J, Lan Z, Jiang S, et al. Telomerase Reverse Transcriptase Preserves Neuron Survival and Cognition in Alzheimer's Disease Models. Nat Aging. 2021;1:1162-1174 pubmed publisher
	Bothe M, Buschow R, Meijsing S. Glucocorticoid signaling induces transcriptional memory and universally reversible chromatin changes. Life Sci Alliance. 2021;4: pubmed publisher
	Mert U, Adawy A, Scharff E, Teichmann P, Willms A, Haselmann V, et al. TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors. Cancers (Basel). 2019;11: pubmed publisher
	Zhang P, Chen Z, Li R, Guo Y, Shi H, Bai J, et al. Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates. Cell Discov. 2018;4:4 pubmed publisher
	Di Blasi C, Bellafiore E, Salih M, Manzini M, Moore S, Seidahmed M, et al. Variable disease severity in Saudi Arabian and Sudanese families with c.3924 + 2 T > C mutation of LAMA2. BMC Res Notes. 2011;4:534 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

Catalog # :

MA1-46093

Quantity :

100 µg

Price :

US 466

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Yeast

Applications :

ChIP Assay: 1-10 µL, CUT&RUN: Assay-dependent, ELISA: 1:100-1:2,000, Immunocytochemistry: 1:50 - 1:100, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 1-5 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:20,000

Species :

Human, Mouse, Yeast

Clone :

4H8

Isotype :

IgG1

Storage :

-20°C

Description :

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

Immunogen :

Chemically synthesized phospho-ser 5 YSPTSpPS (Human).

Format :

Liquid

Applications w/Dilutions :

Aliases :

220kDa; AW321876; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha 1, 180 kDa; DNA polymerase alpha 1, catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DNA polymerase alpha subunit I; DNA polymerase epsilon catalytic subunit; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DUN2; EC 2.7.7.6; G7a/Bat6Hom; hRPB220; hsRPB1; I79_002751; I79_016081; MGC75453; N0825; NSX; p180; Pol II S2p; POL2; Pola; Pola1; POLR2; POLR2A; POLRA; polymerase (DNA directed), alpha 1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; polymerase, alpha 1; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II carboxy-terminal domain; RNA polymerase II C-terminal domain; RNA polymerase II largest subunit; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; Rpb1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8; YNL262W

more info or order :

Invitrogen product webpage