WT1 Monoclonal Antibody (6F-H2) | Invitrogen MA1-46028 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

WT1 Monoclonal Antibody (6F-H2)

catalog :

MA1-46028

quantity :

100 µL

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F-H2

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig 1	Atik Y, Cetinkaya Demir B, Ozan H, Baykara S, Usubutun A, Yılmaz Erturk F. Wilms' tumor 1 protein expression in endometrial adenocarcinoma and endometrial intra-epithelial neoplasia. J Obstet Gynaecol Res. 2016;42:870-5 pubmed publisher
immunocytochemistry; mouse; fig 2	Trembley M, Velasquez L, Small E. Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration. J Vis Exp. 2016;: pubmed publisher
immunohistochemistry - paraffin section; human	Abdelzaher E, Abdallah D. Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study. Biomed Res Int. 2014;2014:968794 pubmed publisher
immunohistochemistry; human; 1:50	Al Salam S, Balalaa N, Faour I, Akhter S, Alashari M. HIF-1?, VEGF and WT-1 are protagonists in bilateral primary angiosarcoma of breast: a case report and review of literature. Int J Clin Exp Pathol. 2012;5:247-53 pubmed
immunohistochemistry; human; 1:50	Hara N, Kawaguchi M, Murayama S, Maruyama R, Tanikawa T, Takahashi K. Mixed epithelial and stromal tumor of the kidney in a 12-year-old girl. Pathol Int. 2005;55:670-6 pubmed
	Rajagopalan A, Venkatesh I, Aslam R, Kirchenbuechler D, Khanna S, Cimbaluk D, et al. SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues. Cell Rep Methods. 2021;1: pubmed publisher
	Anobile D, Bironzo P, Picca F, Lingua M, Morena D, Righi L, et al. Evaluation of the Preclinical Efficacy of Lurbinectedin in Malignant Pleural Mesothelioma. Cancers (Basel). 2021;13: pubmed publisher
	Peng D, Gleyzer R, Tai W, Kumar P, Bian Q, Isaacs B, et al. Evaluating the transcriptional fidelity of cancer models. Genome Med. 2021;13:73 pubmed publisher
	Minocha E, Sinha R, Jain M, Chaturvedi C, Nityanand S. Amniotic fluid stem cells ameliorate cisplatin-induced acute renal failure through induction of autophagy and inhibition of apoptosis. Stem Cell Res Ther. 2019;10:370 pubmed publisher
	Saeed M, Mertens R, Handgretinger R, Efferth T. Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling. Int J Oncol. 2018;53:1721-1731 pubmed publisher

image

image 1 :

image 2 :

Western Blot of WT1 in 293 cell lysate using MA1-46028.

product information

Product Type :

Antibody

Product Name :

WT1 Monoclonal Antibody (6F-H2)

Catalog # :

MA1-46028

Quantity :

100 µL

Price :

US 450.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): 1:400, Immunohistochemistry (Paraffin): 1:400, Immunoprecipitation: 2-10 µg/mL lysate, Western Blot: 2 µg/mL

Species :

Human

Clone :

6F-H2

Isotype :

IgG1, kappa

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

Immunogen :

Recombinant protein corresponding to residues 1-181 of human WT1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): 1:400, Immunohistochemistry (Paraffin): 1:400, Immunoprecipitation: 2-10 µg/mL lysate, Western Blot: 2 µg/mL

Aliases :

AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms tumor 1 homolog; Wilms tumor protein; Wilms tumor protein homolog; Wilms tumor protein isoform Ex4a(+); WIT1; WIT-1; WIT-2; WT; Wt1; Wt-1; WT1 antisense RNA; WT1AS; WT1-AS; WT1-AS1; WT33

more info or order :

Invitrogen product webpage