APE1 Monoclonal Antibody (13B 8E5C2) | Invitrogen MA1-440 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

APE1 Monoclonal Antibody (13B 8E5C2)

catalog :

MA1-440

quantity :

100 uL

price :

US 491.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B 8E5C2

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, RNA immunoprecipitation

citations: 8

Reference
Schwarz S, Xu J, Gunasekera K, Sch xfc rmann D, V xe5 gb xf8 C, Ferrari E, et al. Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation. Nat Commun. 2024;15:184 pubmed publisher
Ballout F, Lu H, Chen L, Sriramajayam K, Que J, Meng Z, et al. APE1 redox function is required for activation of Yes-associated protein 1 under reflux conditions in Barrett's-associated esophageal adenocarcinomas. J Exp Clin Cancer Res. 2022;41:264 pubmed publisher
lvarez Quil n A, Wojtaszek J, Mathieu M, Patel T, Appel C, Hustedt N, et al. Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease. Mol Cell. 2020;78:1152-1165.e8 pubmed publisher
Lu H, Bhat A, Peng D, Chen Z, Zhu S, Hong J, et al. APE1 Upregulates MMP-14 via Redox-Sensitive ARF6-Mediated Recycling to Promote Cell Invasion of Esophageal Adenocarcinoma. Cancer Res. 2019;79:4426-4438 pubmed publisher
Bhat A, Lu H, Soutto M, Capobianco A, Rai P, Zaika A, et al. Exposure of Barrett's and esophageal adenocarcinoma cells to bile acids activates EGFR-STAT3 signaling axis via induction of APE1. Oncogene. 2018;37:6011-6024 pubmed publisher
Freitas S, Moore D, Michael H, Kelley M. Studies of apurinic/apyrimidinic endonuclease/ref-1 expression in epithelial ovarian cancer: correlations with tumor progression and platinum resistance. Clin Cancer Res. 2003;9:4689-94 pubmed
Robertson K, Hill D, Xu Y, Liu L, Van Epps S, Hockenbery D, et al. Down-regulation of apurinic/apyrimidinic endonuclease expression is associated with the induction of apoptosis in differentiating myeloid leukemia cells. Cell Growth Differ. 1997;8:443-9 pubmed
Duguid J, Eble J, Wilson T, Kelley M. Differential cellular and subcellular expression of the human multifunctional apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme. Cancer Res. 1995;55:6097-102 pubmed

product information

Product Type :

Antibody

Product Name :

APE1 Monoclonal Antibody (13B 8E5C2)

Catalog # :

MA1-440

Quantity :

100 uL

Price :

US 491.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Non-human primate, Rat

Applications :

ChIP Assay: 1.5 ug/10^6 cells, Flow Cytometry: 2-5 ug/mL, Immunocytochemistry: 1:50-1:500, Immunohistochemistry (Frozen): 1/5000, Immunohistochemistry (Paraffin): 1:50-1:1,000, Immunoprecipitation: 5 ug, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Canine, Human, Mouse, Non-human primate, Rat

Clone :

13B 8E5C2

Isotype :

IgG2a

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

Immunogen :

Full-length recombinant human APE protein.

Format :

Liquid

Applications w/Dilutions :

Aliases :

AP endonuclease 1; AP endonuclease class I; AP lyase; Ape; APE1; APEN; Apex; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apex1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endodeoxyribonuclease 1; apurinic/apyrimidinic endonuclease; apurinic/apyrimidinic endonuclease 1; Apurinic-apyrimidinic endonuclease 1; APX; BAP 1; BAP1; deoxyribonuclease (apurinic or apyrimidinic); DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase, mitochondrial; HAP1; protein REF-1; redox factor 1; redox factor-1; REF1; REF-1