Phospho-Progesterone Receptor (Ser294) Monoclonal Antibody (608)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Progesterone Receptor (Ser294) Monoclonal Antibody (608)

catalog :

MA1-414

quantity :

200 µg

price :

US 420.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

608

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	Hagan C, Knutson T, Lange C. A Common Docking Domain in Progesterone Receptor-B links DUSP6 and CK2 signaling to proliferative transcriptional programs in breast cancer cells. Nucleic Acids Res. 2013;41:8926-42 pubmed publisher
immunocytochemistry; mouse; 1:100	Bottino M, Cerliani J, Rojas P, Giulianelli S, Soldati R, Mondillo C, et al. Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects. Breast Cancer Res Treat. 2011;126:621-36 pubmed publisher
immunohistochemistry; rat; 1:300	Gordon A, Aguilar R, Garrido Gracia J, Guil Luna S, Sánchez Cespedes R, Millan Y, et al. Immunoneutralization of inhibin in cycling rats increases follicle-stimulating hormone secretion, stimulates the ovary and attenuates progesterone receptor-dependent preovulatory luteinizing hormone secretion. Neuroendocrinology. 2010;91:291-301 pubmed publisher
western blot; human	Daniel A, Lange C. Protein kinases mediate ligand-independent derepression of sumoylated progesterone receptors in breast cancer cells. Proc Natl Acad Sci U S A. 2009;106:14287-92 pubmed publisher
western blot; human	Proietti C, Rosemblit C, Beguelin W, Rivas M, Díaz Flaqué M, Charreau E, et al. Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth. Mol Cell Biol. 2009;29:1249-65 pubmed publisher
western blot; mouse; 1:50	Giulianelli S, Cerliani J, Lamb C, Fabris V, Bottino M, Gorostiaga M, et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int J Cancer. 2008;123:2518-31 pubmed publisher
western blot; human	Daniel A, Qiu M, Faivre E, Ostrander J, Skildum A, Lange C. Linkage of progestin and epidermal growth factor signaling: phosphorylation of progesterone receptors mediates transcriptional hypersensitivity and increased ligand-independent breast cancer cell growth. Steroids. 2007;72:188-201 pubmed
western blot; human	Skildum A, Faivre E, Lange C. Progesterone receptors induce cell cycle progression via activation of mitogen-activated protein kinases. Mol Endocrinol. 2005;19:327-39 pubmed
	Salatino M, Schillaci R, Proietti C, Carnevale R, Frahm I, Molinolo A, et al. Inhibition of in vivo breast cancer growth by antisense oligodeoxynucleotides to type I insulin-like growth factor receptor mRNA involves inactivation of ErbBs, PI-3K/Akt and p42/p44 MAPK signaling pathways but not modulation of progesterone receptor. Oncogene. 2004;23:5161-74 pubmed
	Qiu M, Olsen A, Faivre E, Horwitz K, Lange C. Mitogen-activated protein kinase regulates nuclear association of human progesterone receptors. Mol Endocrinol. 2003;17:628-42 pubmed
	Clemm D, Sherman L, Boonyaratanakornkit V, Schrader W, Weigel N, Edwards D. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol. 2000;14:52-65 pubmed

image

image 1 :

Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in U251 Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in Hela Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 3 :

Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in MCF-7 Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Phospho-Progesterone Receptor (Ser294) Monoclonal Antibody (608)

Catalog # :

MA1-414

Quantity :

200 µg

Price :

US 420.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 10 µg/mL

Species :

Human

Clone :

608

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

Immunogen :

Synthetic phosphopeptide corresponding to residues P(288) M A P G R S(p) P L A T T V(300) of human Progesterone Receptor.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 10 µg/mL

Aliases :

9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product

more info or order :

Invitrogen product webpage