Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)

catalog :

MA1-413

quantity :

100 µg

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

1154

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:100	Bottino M, Cerliani J, Rojas P, Giulianelli S, Soldati R, Mondillo C, et al. Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects. Breast Cancer Res Treat. 2011;126:621-36 pubmed publisher
western blot; human	Quiles I, Millán Ariño L, Subtil Rodríguez A, Minana B, Spinedi N, Ballaré C, et al. Mutational analysis of progesterone receptor functional domains in stable cell lines delineates sets of genes regulated by different mechanisms. Mol Endocrinol. 2009;23:809-26 pubmed publisher
western blot; mouse; 1:50	Giulianelli S, Cerliani J, Lamb C, Fabris V, Bottino M, Gorostiaga M, et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int J Cancer. 2008;123:2518-31 pubmed publisher
immunoprecipitation; human immunocytochemistry; human	Yang F, Camp D, Gritsenko M, Luo Q, Kelly R, Clauss T, et al. Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. J Cell Sci. 2007;120:4060-70 pubmed
	Clemm D, Sherman L, Boonyaratanakornkit V, Schrader W, Weigel N, Edwards D. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol. 2000;14:52-65 pubmed

image

image 1 :

Immunofluorescent analysis of Phospho-Progesterone Receptor pSer190 in untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-Progesterone Receptor pSer190 Monclonal Antibody (1154) (Product # MA1-413) at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

product information

Product Type :

Antibody

Product Name :

Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)

Catalog # :

MA1-413

Quantity :

100 µg

Price :

US 450.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 4 µg/mL

Species :

Human

Clone :

1154

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

Immunogen :

Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 4 µg/mL

Aliases :

9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product

more info or order :

Invitrogen product webpage