Caspase 8 Monoclonal Antibody (90A992) | Invitrogen MA1-41280 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Caspase 8 Monoclonal Antibody (90A992)

catalog :

MA1-41280

quantity :

100 µg

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

90A992

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 5b	Yu G, Dou Z, Jia Z. 5?bromo?3?(3?hydroxyprop?1?ynyl)?2H?pyran?2?one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways. Mol Med Rep. 2017;15:153-159 pubmed publisher
western blot; human; fig 2	Arora R, Sawney S, Saini V, Steffi C, Tiwari M, Saluja D. Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1. Mol Cancer. 2016;15:64 pubmed
immunohistochemistry; human; fig 3a	Malherbe J, Fuller K, Mirzai B, Kavanagh S, So C, Ip H, et al. Dysregulation of the intrinsic apoptotic pathway mediates megakaryocytic hyperplasia in myeloproliferative neoplasms. J Clin Pathol. 2016;: pubmed publisher
	Mlyczy x144 ska E, Myszka M, Kurowska P, Dawid M, Milewicz T, Ba x142 ajewicz Nowak M, et al. Anti-Apoptotic Effect of Apelin in Human Placenta: Studies on BeWo Cells and Villous Explants from Third-Trimester Human Pregnancy. Int J Mol Sci. 2021;22: pubmed publisher
	Noonan A, Cousins A, Anderson D, Zeligs K, Bunch K, Hernandez L, et al. Matrix Drug Screen Identifies Synergistic Drug Combinations to Augment SMAC Mimetic Activity in Ovarian Cancer. Cancers (Basel). 2020;12: pubmed publisher
	Kurowska P, Mlyczynska E, Dawid M, Opydo Chanek M, Dupont J, Rak A. In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3. Int J Mol Sci. 2019;20: pubmed publisher
	Zhao X, Zhang C, Le Z, Zeng S, Pan C, Shi J, et al. Telomerase reverse transcriptase interference synergistically promotes tumor necrosis factor‑related apoptosis‑inducing ligand‑induced oral squamous cell carcinoma apoptosis and suppresses proliferation in vitro and in vivo. Int J Mol Med. 2018;42:1283-1294 pubmed publisher

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product information

Product Type :

Antibody

Product Name :

Caspase 8 Monoclonal Antibody (90A992)

Catalog # :

MA1-41280

Quantity :

100 µg

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Non-human primate

Applications :

Flow Cytometry: 0.1-0.5 µg/mL, Immunohistochemistry (Paraffin): 4 µg/mL, Western Blot: 0.5-2 µg/mL

Species :

Human, Non-human primate

Clone :

90A992

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

Immunogen :

Amino acids 360-385 of the human Caspase 8 protein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.1-0.5 µg/mL, Immunohistochemistry (Paraffin): 4 µg/mL, Western Blot: 0.5-2 µg/mL

Aliases :

ALPS2B; apoptosis-related cysteine peptidase; apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP8; CASP-8; caspase 8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase8; caspase-8; Caspase-8 precursor; Caspase-8 subunit p10; Caspase-8 subunit p18; EC 3.4.22.61; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; FLICE; FLJ17672; ICE8; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581

more info or order :

Invitrogen product webpage