This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ROCK1 (Cleaved Asp1113) Monoclonal Antibody (154C1465)
catalog :
MA1-41031
quantity :
100 ug
price :
US 480.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
154C1465

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, domestic rabbit
application :
western blot, immunocytochemistry
citations: 1
Reference
Ferrer B, Prince L, Tinkov A, Santamaria A, Farina M, Rocha J, et al. Chronic exposure to methylmercury enhances the anorexigenic effects of leptin in C57BL/6J male mice. Food Chem Toxicol. 2021;147:111924 pubmed publisher
product information
Product Type :
Antibody
Product Name :
ROCK1 (Cleaved Asp1113) Monoclonal Antibody (154C1465)
Catalog # :
MA1-41031
Quantity :
100 ug
Price :
US 480.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
Applications :
Immunocytochemistry: Assay-Dependent, Western Blot: 1-2 ug/mL
Species :
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
Clone :
154C1465
Isotype :
IgG
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
Immunogen :
Synthetic peptide corresponding to residues G(1114) N L P E S R I E G W L S(1126) of human ROCK-1.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: Assay-Dependent, Western Blot: 1-2 ug/mL
Aliases :
1110055K06Rik; Ac2-154; cAMP-dependent protein kinase ROCK-I; cePKA; corneal epithelial Rho-associated-ser/thr kinase; corneal epithelial Rho-associated-Ser/Thr kinase 1; HEBM1; liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160ROCK; PRO0435; renal carcinoma antigen NY-REN-35; Rho associated coiled-coil containing protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK1; Rock-I
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA