NOXA Monoclonal Antibody (114C307.1) | Invitrogen MA1-41000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NOXA Monoclonal Antibody (114C307.1)

catalog :

MA1-41000

quantity :

100µg

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

114C307.1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2c	Suzuki J, Nakajima W, Suzuki H, Asano Y, Tanaka N. Chaperone-mediated autophagy promotes lung cancer cell survival through selective stabilization of the pro-survival protein, MCL1. Biochem Biophys Res Commun. 2017;482:1334-1340 pubmed publisher
western blot; human; loading ...; fig 1b	Nakajima W, Sharma K, Lee J, Maxim N, Hicks M, Vu T, et al. DNA damaging agent-induced apoptosis is regulated by MCL-1 phosphorylation and degradation mediated by the Noxa/MCL-1/CDK2 complex. Oncotarget. 2016;7:36353-36365 pubmed publisher
western blot; human; loading ...; fig 1a	Matsumoto M, Nakajima W, Seike M, Gemma A, Tanaka N. Cisplatin-induced apoptosis in non-small-cell lung cancer cells is dependent on Bax- and Bak-induction pathway and synergistically activated by BH3-mimetic ABT-263 in p53 wild-type and mutant cells. Biochem Biophys Res Commun. 2016;473:490-6 pubmed publisher
western blot; human; loading ...; fig 1	Nakajima W, Sharma K, Hicks M, Le N, Brown R, Krystal G, et al. Combination with vorinostat overcomes ABT-263 (navitoclax) resistance of small cell lung cancer. Cancer Biol Ther. 2016;17:27-35 pubmed publisher
western blot; human	Nakajima W, Hicks M, Tanaka N, Krystal G, Harada H. Noxa determines localization and stability of MCL-1 and consequently ABT-737 sensitivity in small cell lung cancer. Cell Death Dis. 2014;5:e1052 pubmed publisher
western blot; human	Pandey M, Gowda K, Doi K, Sharma A, Wang H, Amin S. Proteasomal degradation of Mcl-1 by maritoclax induces apoptosis and enhances the efficacy of ABT-737 in melanoma cells. PLoS ONE. 2013;8:e78570 pubmed publisher
western blot; human	Vin H, Ojeda S, Ching G, Leung M, Chitsazzadeh V, Dwyer D, et al. BRAF inhibitors suppress apoptosis through off-target inhibition of JNK signaling. elife. 2013;2:e00969 pubmed publisher
	Britt E, Raman S, Leek K, Sheehy C, KIM S, Harada H. Combination of fenretinide and ABT-263 induces apoptosis through NOXA for head and neck squamous cell carcinoma treatment. PLoS ONE. 2019;14:e0219398 pubmed publisher
	Sharma K, Vu T, Cook W, Naseri M, Zhan K, Nakajima W, et al. p53-independent Noxa induction by cisplatin is regulated by ATF3/ATF4 in head and neck squamous cell carcinoma cells. Mol Oncol. 2018;12:788-798 pubmed publisher
	Lochmann T, Floros K, Naseri M, Powell K, Cook W, March R, et al. Venetoclax Is Effective in Small-Cell Lung Cancers with High BCL-2 Expression. Clin Cancer Res. 2018;24:360-369 pubmed publisher

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product information

Product Type :

Antibody

Product Name :

NOXA Monoclonal Antibody (114C307.1)

Catalog # :

MA1-41000

Quantity :

100µg

Price :

US 390

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:20-1:1000, Immunofluorescence: 1:20-1:1000, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Mouse, Rat

Clone :

114C307.1

Isotype :

IgG1, kappa

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.

Immunogen :

Human Noxa.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:20-1:1000, Immunofluorescence: 1:20-1:1000, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

adult T cell leukemia-derived PMA-responsive; APR; Immediate-early-response protein APR; NOXA; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-ind; PMA-induced protein 1; Pmaip1; Protein Noxa

more info or order :

Invitrogen product webpage