CD8 Monoclonal Antibody (SP16) | Invitrogen MA1-39566 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD8 Monoclonal Antibody (SP16)

catalog :

MA1-39566

quantity :

1 mL

price :

US 696

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP16

reactivity :

human, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 43

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 4a	Biasci D, Smoragiewicz M, Connell C, Wang Z, Gao Y, Thaventhiran J, et al. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response. Proc Natl Acad Sci U S A. 2020;117:28960-28970 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1e	Noh B, Kwak J, Eom D. Immune classification for the PD-L1 expression and tumour-infiltrating lymphocytes in colorectal adenocarcinoma. BMC Cancer. 2020;20:58 pubmed publisher
immunohistochemistry; human; loading ...; fig 4d	Smillie C, Biton M, Ordovas Montanes J, Sullivan K, Burgin G, Graham D, et al. Intra- and Inter-cellular Rewiring of the Human Colon during Ulcerative Colitis. Cell. 2019;178:714-730.e22 pubmed publisher
immunohistochemistry; human; loading ...; fig 3a	Garcia Hernandez M, Uribe Uribe N, Espinosa González R, Kast W, Khader S, Rangel Moreno J. A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression. Front Immunol. 2017;8:563 pubmed publisher
immunohistochemistry - paraffin section; human	Kim W, Jung H, Nam S, Kim T, Heo D, Kim C, et al. Expression of programmed cell death ligand 1 (PD-L1) in advanced stage EBV-associated extranodal NK/T cell lymphoma is associated with better prognosis. Virchows Arch. 2016;469:581-590 pubmed
immunohistochemistry - frozen section; human; 1:100; loading ...; tbl s2	Leong Y, Chen Y, Ong H, Wu D, Man K, Deléage C, et al. CXCR5(+) follicular cytotoxic T cells control viral infection in B cell follicles. Nat Immunol. 2016;17:1187-96 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Kim K, Wen X, Yang H, Kim W, Kang G. Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma. PLoS ONE. 2015;10:e0144192 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 1	Liu T, Weng S, Wang M, Huang W. Nontuberculous mycobacterial infection with concurrent IgG4-related lymphadenopathy. APMIS. 2016;124:216-20 pubmed publisher
immunohistochemistry - paraffin section; human; 1:150; fig 1	Liu K, Yang K, Wu B, Chen H, Chen X, Chen X, et al. Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer. Medicine (Baltimore). 2015;94:e1631 pubmed publisher
immunohistochemistry - paraffin section; human	Jeon Y, Kim J, Sung J, Han J, Ko Y. Epstein-Barr virus-positive nodal T/NK-cell lymphoma: an analysis of 15 cases with distinct clinicopathological features. Hum Pathol. 2015;46:981-90 pubmed publisher
immunohistochemistry; human	Kim M, Koh J, Kim S, Go H, Jeon Y, Chung D. Clinicopathological analysis of PD-L1 and PD-L2 expression in pulmonary squamous cell carcinoma: Comparison with tumor-infiltrating T cells and the status of oncogenic drivers. Lung Cancer. 2015;88:24-33 pubmed publisher
immunohistochemistry - paraffin section; human	Novinger L, Ashikaga T, Krag D. Identification of tumor-binding scFv derived from clonally related B cells in tumor and lymph node of a patient with breast cancer. Cancer Immunol Immunother. 2015;64:29-39 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Yuan Z, Luo R, Peng R, Wang S, Xue C. High infiltration of tumor-associated macrophages in triple-negative breast cancer is associated with a higher risk of distant metastasis. Onco Targets Ther. 2014;7:1475-80 pubmed publisher
immunohistochemistry; human; 1:50	Fan Y, Hu S, Liu J, Xiao F, Li X, Yu W, et al. Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion. Mediators Inflamm. 2014;2014:397815 pubmed publisher
immunohistochemistry - paraffin section; human	Rizzo S, Basso C, Troost D, Aronica E, Frigo A, Driessen A, et al. T-cell-mediated inflammatory activity in the stellate ganglia of patients with ion-channel disease and severe ventricular arrhythmias. Circ Arrhythm Electrophysiol. 2014;7:224-9 pubmed publisher
immunohistochemistry; human; 1:50	Sereti I, Estes J, Thompson W, Morcock D, Fischl M, Croughs T, et al. Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration. PLoS Pathog. 2014;10:e1003890 pubmed publisher
immunohistochemistry; human; 1:250	Pohl M, Kawakami N, Kitic M, Bauer J, Martins R, Fischer M, et al. T cell-activation in neuromyelitis optica lesions plays a role in their formation. Acta Neuropathol Commun. 2013;1:85 pubmed publisher
immunocytochemistry; human	Kühnemuth B, Mühlberg L, Schipper M, Griesmann H, Neesse A, Milosevic N, et al. CUX1 modulates polarization of tumor-associated macrophages by antagonizing NF-?B signaling. Oncogene. 2015;34:177-87 pubmed publisher
immunohistochemistry; human; 1:100	Kim K, Lee K, Cho H, Kim Y, Yang H, Kim W, et al. Prognostic implications of tumor-infiltrating FoxP3+ regulatory T cells and CD8+ cytotoxic T cells in microsatellite-unstable gastric cancers. Hum Pathol. 2014;45:285-93 pubmed publisher
immunohistochemistry; human; 1:25	Abramov D, Oschlies I, Zimmermann M, Konovalov D, Damm Welk C, Wössmann W, et al. Expression of CD8 is associated with non-common type morphology and outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma. Haematologica. 2013;98:1547-53 pubmed publisher
immunohistochemistry; human	Kim S, Jeong H, Woo O, Seo J, Kim A, Lee E, et al. Tumor-infiltrating lymphocytes, tumor characteristics, and recurrence in patients with early breast cancer. Am J Clin Oncol. 2013;36:224-31 pubmed publisher
immunohistochemistry; human; 1:100; fig 3	Zeng M, Southern P, Reilly C, Beilman G, Chipman J, Schacker T, et al. Lymphoid tissue damage in HIV-1 infection depletes naïve T cells and limits T cell reconstitution after antiretroviral therapy. PLoS Pathog. 2012;8:e1002437 pubmed publisher
immunohistochemistry; human; 1:100	West N, Milne K, Truong P, MacPherson N, Nelson B, Watson P. Tumor-infiltrating lymphocytes predict response to anthracycline-based chemotherapy in estrogen receptor-negative breast cancer. Breast Cancer Res. 2011;13:R126 pubmed publisher
immunohistochemistry; human; 1:100	Chang I, Chen H, Ma M, Huang W. Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases. APMIS. 2011;119:794-801 pubmed publisher
immunohistochemistry; human; 1:50	Caramori G, Lasagna L, Casalini A, Adcock I, Casolari P, Contoli M, et al. Immune response to Mycobacterium tuberculosis infection in the parietal pleura of patients with tuberculous pleurisy. PLoS ONE. 2011;6:e22637 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; tbl 2	West N, Panet Raymond V, Truong P, Alexander C, Babinszky S, Milne K, et al. Intratumoral Immune Responses Can Distinguish New Primary and True Recurrence Types of Ipsilateral Breast Tumor Recurrences (IBTR). Breast Cancer (Auckl). 2011;5:105-15 pubmed publisher
immunohistochemistry; human	Piet B, de Bree G, Smids Dierdorp B, van der Loos C, Remmerswaal E, von der Thüsen J, et al. CD8? T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung. J Clin Invest. 2011;121:2254-63 pubmed publisher
immunohistochemistry; human	de Boer O, Teeling P, Jansen M, Ploegmakers H, van der Loos C, Kummer J, et al. Spatial differences in the presence of FOXP3+ and GranzymeB+ T cells between the intra- and extravascular compartments in renal allograft vasculopathy. PLoS ONE. 2011;6:e18656 pubmed publisher
immunohistochemistry; human; 1:50	Riezu Boj J, Larrea E, Aldabe R, Guembe L, Casares N, Galeano E, et al. Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection. J Hepatol. 2011;54:422-31 pubmed publisher
immunohistochemistry; human	Sonar S, Hsu Y, Conrad M, Majeau G, Kilic A, Garber E, et al. Antagonism of TIM-1 blocks the development of disease in a humanized mouse model of allergic asthma. J Clin Invest. 2010;120:2767-81 pubmed publisher
immunohistochemistry; human; 1:200	Gualco G, Wludarski S, Hayashi Silva L, Medeiros Filho P, Veras G, Bacchi C. Primary central nervous system peripheral T-cell lymphoma in a child. Fetal Pediatr Pathol. 2010;29:224-30 pubmed publisher
immunohistochemistry; human; 1:100	Prabhu S, Gupta P, Durgapal H, Rath S, Gupta S, Acharya S, et al. Study of cellular immune response against Hepatitis E virus (HEV). J Viral Hepat. 2011;18:587-94 pubmed publisher
immunohistochemistry; human	Imanguli M, Swaim W, League S, Gress R, Pavletic S, Hakim F. Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa. Blood. 2009;113:3620-30 pubmed publisher
immunohistochemistry; human; 1:100; fig 1	Milne K, Barnes R, Girardin A, Mawer M, Nesslinger N, Ng A, et al. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer. PLoS ONE. 2008;3:e3409 pubmed publisher
immunohistochemistry; human	van Es L, De Heer E, Vleming L, van der Wal A, Mallat M, Bajema I, et al. GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy. Kidney Int. 2008;73:1426-33 pubmed publisher
immunohistochemistry; human	Keller T, van der Meer J, Teeling P, van der Sluijs K, Idu M, Rimmelzwaan G, et al. Selective expansion of influenza A virus-specific T cells in symptomatic human carotid artery atherosclerotic plaques. Stroke. 2008;39:174-9 pubmed
immunohistochemistry; rat	Avunduk A, Avunduk M, Baltaci A, Mogulkoc R. Effect of melatonin and zinc on the immune response in experimental Toxoplasma retinochoroiditis. Ophthalmologica. 2007;221:421-5 pubmed
immunohistochemistry; human	Abel M, Sene D, Pol S, Bourliere M, Poynard T, Charlotte F, et al. Intrahepatic virus-specific IL-10-producing CD8 T cells prevent liver damage during chronic hepatitis C virus infection. Hepatology. 2006;44:1607-16 pubmed
	Ac xfa rcio R, Pozzi S, Carreira B, Pojo M, G xf3 mez Cebri xe1 n N, Casimiro S, et al. Therapeutic targeting of PD-1/PD-L1 blockade by novel small-molecule inhibitors recruits cytotoxic T cells into solid tumor microenvironment. J Immunother Cancer. 2022;10: pubmed publisher
	Ludwig B, Ludwig S, Steffen A, Knauf Y, Zimerman B, Heinke S, et al. Favorable outcome of experimental islet xenotransplantation without immunosuppression in a nonhuman primate model of diabetes. Proc Natl Acad Sci U S A. 2017;114:11745-11750 pubmed publisher
	Koh J, Kim S, Kim M, Go H, Jeon Y, Chung D. Prognostic implications of intratumoral CD103+ tumor-infiltrating lymphocytes in pulmonary squamous cell carcinoma. Oncotarget. 2017;8:13762-13769 pubmed publisher
	Sun H, Xu J, Huang M, Huang Q, Sun R, Xiao W, et al. CD200R, a co-inhibitory receptor on immune cells, predicts the prognosis of human hepatocellular carcinoma. Immunol Lett. 2016;178:105-13 pubmed publisher
	Deléage C, Schuetz A, Alvord W, Johnston L, Hao X, Morcock D, et al. Impact of early cART in the gut during acute HIV infection. JCI Insight. 2016;1: pubmed

product information

Product Type :

Antibody

Product Name :

CD8 Monoclonal Antibody (SP16)

Catalog # :

MA1-39566

Quantity :

1 mL

Price :

US 696

Clonality :

Monoclonal

Host :

Rabbit

Reactivity :

Human

Applications :

Flow Cytometry: 1:1,000, Immunohistochemistry (Paraffin): 1:100

Species :

Human

Clone :

SP16

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Immunogen :

Synthetic peptide corresponding to the C-terminus of alpha chain of the human CD8 molecule

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:1,000, Immunohistochemistry (Paraffin): 1:100

Aliases :

BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein