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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
PMEL Monoclonal Antibody (HMB45)
catalog :
MA1-34759
quantity :
500 uL
price :
US 524.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HMB45
reactivity :
human, mouse, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 54
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; fig 1c
Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 1
Chen G, Luo Y, Eriksson D, Meng X, Qian C, Bauerle T, et al. High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6. Oncotarget. 2016;7:26653-69 pubmed publisher
  • immunohistochemistry; human; 1:80; fig 10
Costache M, Dumitru A, Pătraşcu O, Popa Cherecheanu D, Bădilă P, Miu J, et al. A challenging case of ocular melanoma. Rom J Morphol Embryol. 2015;56:817-22 pubmed
  • western blot; mouse
Woan K, Lienlaf M, Perez Villaroel P, Lee C, Cheng F, Knox T, et al. Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation. Mol Oncol. 2015;9:1447-1457 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:80
Costache M, Pătraşcu O, Dumitru A, Costache D, Voinea L, Simionescu O, et al. Histopathological findings concerning ocular melanomas. Rom J Morphol Embryol. 2014;55:649-53 pubmed
  • immunohistochemistry; human
Jabbour M, Seoud M, Al Ahmadie H, Abdul Karim F, Zaatari G. ASPL-TFE3 translocation in vulvovaginal alveolar soft part sarcoma. Int J Gynecol Pathol. 2014;33:263-7 pubmed publisher
  • immunocytochemistry; human; 1:50
  • western blot; human
Leonhardt R, Vigneron N, Hee J, Graham M, Cresswell P. Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly of melanosomal fibrils. Mol Biol Cell. 2013;24:964-81 pubmed publisher
  • immunohistochemistry; human; 1:100
Kim B, Bae Y, Kim S, Jeong H, Hong S, Yoon S. Usefulness of Ki-67 (MIB-1) immunostaining in the diagnosis of pulmonary sclerosing hemangiomas. APMIS. 2013;121:105-10 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:40
Niu H, Wang F, Zhang P, Bing Z. Cardiac Epithelioid PEComa: Report of Two Cases and Review of the Literature. Case Rep Med. 2012;2012:521678 pubmed publisher
  • immunohistochemistry; human; 1:40
Wang Z, Gong Q, Fan Q. Expression of E-cadherin in angiomyolipoma. Hum Pathol. 2012;43:2348-53 pubmed publisher
  • immunohistochemistry; human
Zhou Z, Yu M, Yang S, Zhou J, Sun R, Yang G. Dural angioleiomyoma of the middle cranial fossa: a case report and review of the literature. Brain Tumor Pathol. 2013;30:117-21 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
Hooijkaas A, Gadiot J, van der Valk M, Mooi W, Blank C. Targeting BRAFV600E in an inducible murine model of melanoma. Am J Pathol. 2012;181:785-94 pubmed publisher
  • immunocytochemistry; mouse
Sitaram A, Dennis M, Chaudhuri R, De Jesus Rojas W, Tenza D, Setty S, et al. Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes. Mol Biol Cell. 2012;23:3178-92 pubmed
  • immunohistochemistry; human; 1:200
Duong T, Proulx S, Luciani P, Leroux J, Detmar M, Koopman P, et al. Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice. Cancer Res. 2012;72:3105-14 pubmed publisher
  • immunohistochemistry; human; 1:50
Yin W, Li J, Chan J. Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review. Diagn Pathol. 2012;7:39 pubmed publisher
  • immunohistochemistry; human
Anbar T, El Sawy A, Attia S, Barakat M, Moftah N, El Ammawy T, et al. Effect of PUVA therapy on melanocytes and keratinocytes in non-segmental vitiligo: histopathological, immuno-histochemical and ultrastructural study. Photodermatol Photoimmunol Photomed. 2012;28:17-25 pubmed publisher
  • western blot; mouse
WATT B, Tenza D, Lemmon M, Kerje S, Raposo G, Andersson L, et al. Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic. PLoS Genet. 2011;7:e1002286 pubmed publisher
  • western blot; human
  • immunocytochemistry; mouse
Hellström A, WATT B, Fard S, Tenza D, Mannstrom P, Narfstrom K, et al. Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation. PLoS Genet. 2011;7:e1002285 pubmed publisher
  • immunocytochemistry; human; 1:300
Westbroek W, Klar A, Cullinane A, Ziegler S, Hurvitz H, Ganem A, et al. Cellular and clinical report of new Griscelli syndrome type III cases. Pigment Cell Melanoma Res. 2012;25:47-56 pubmed publisher
  • immunohistochemistry; human; 1:100
Jabbour M, Fedda F, Tawil A, Shabb N, Boulos F. Follicular dendritic cell sarcoma of the head and neck expressing thyroid transcription factor-1: a case report with clinicopathologic and immunohistochemical literature review. Appl Immunohistochem Mol Morphol. 2014;22:705-12 pubmed publisher
  • immunocytochemistry; human; 1:100
  • western blot; mouse
Beaumont K, Hamilton N, Moores M, Brown D, Ohbayashi N, Cairncross O, et al. The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking. Traffic. 2011;12:627-43 pubmed publisher
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 1
Leonhardt R, Vigneron N, Rahner C, Cresswell P. Proprotein convertases process Pmel17 during secretion. J Biol Chem. 2011;286:9321-37 pubmed publisher
  • immunohistochemistry; human; 1:150
Clay M, Gibson P, Lowell J, Cooper K. Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm. Int J Gynecol Pathol. 2011;30:71-5 pubmed publisher
  • immunohistochemistry; human; 1:100
Yu G, Qu G, Kong L, Pan X, Wang W, Lv J. Primary myoepithelial carcinoma of the larynx: case report and review of the literature. Pathol Res Pract. 2011;207:127-30 pubmed publisher
  • immunocytochemistry; human; 1:800
Bean S, Baker A, Eloubeidi M, Eltoum I, Jhala N, Crowe R, et al. Endoscopic ultrasound-guided fine-needle aspiration of intrathoracic and intra-abdominal spindle cell and mesenchymal lesions. Cancer Cytopathol. 2011;119:37-48 pubmed publisher
  • immunocytochemistry; human; 1:200
Huizing M, Pederson B, Hess R, Griffin A, Helip Wooley A, Westbroek W, et al. Clinical and cellular characterisation of Hermansky-Pudlak syndrome type 6. J Med Genet. 2009;46:803-10 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
WATT B, van Niel G, Fowler D, Hurbain I, Luk K, Stayrook S, et al. N-terminal domains elicit formation of functional Pmel17 amyloid fibrils. J Biol Chem. 2009;284:35543-55 pubmed publisher
  • immunocytochemistry; mouse
Truschel S, Simoes S, Setty S, Harper D, Tenza D, Thomas P, et al. ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane. Traffic. 2009;10:1318-36 pubmed publisher
  • immunohistochemistry; human
Zurac S, Andrei R, Petsakos G, Nichita L, Bastian A, Micu G, et al. Cutaneous metastases of malignant melanoma--how difficult can it be?. Rom J Intern Med. 2008;46:375-8 pubmed
  • immunohistochemistry; human
Anbar T, El Sawy A, Attia S, Moftah N, El Tonsy M. Patterns of repigmentation in two cases of hypopigmented type of vitiligo. Photodermatol Photoimmunol Photomed. 2009;25:156-8 pubmed publisher
  • immunohistochemistry; human
Mukhopadhyay S, El Zammar O, Katzenstein A. Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity. Am J Surg Pathol. 2009;33:487-95 pubmed publisher
  • western blot; mouse
Setty S, Tenza D, Sviderskaya E, Bennett D, Raposo G, Marks M. Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes. Nature. 2008;454:1142-6 pubmed publisher
  • immunohistochemistry; mouse
Tabata H, Kawamura N, Sun Wada G, Wada Y. Vacuolar-type H(+)-ATPase with the a3 isoform is the proton pump on premature melanosomes. Cell Tissue Res. 2008;332:447-60 pubmed publisher
  • immunocytochemistry; human; 1:100
Westbroek W, Tuchman M, Tinloy B, De Wever O, Vilboux T, Hertz J, et al. A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome. Mol Genet Metab. 2008;94:248-54 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Groux Degroote S, van Dijk S, Wolthoorn J, Neumann S, Theos A, De Mazière A, et al. Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants. Traffic. 2008;9:951-63 pubmed publisher
  • western blot; human; 1:500
Roberts D, Newton R, Leonard J, Sturm R. Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes. J Cell Physiol. 2008;215:344-55 pubmed
  • immunohistochemistry; bovine
Oruc E. Melanosis in a Holstein heifer. J Vet Med A Physiol Pathol Clin Med. 2007;54:501-3 pubmed
  • western blot; human
Kawakami A, Sakane F, Imai S, Yasuda S, Kai M, Kanoh H, et al. Rab7 regulates maturation of melanosomal matrix protein gp100/Pmel17/Silv. J Invest Dermatol. 2008;128:143-50 pubmed
  • immunohistochemistry; human
Westbroek W, Adams D, Huizing M, Koshoffer A, Dorward H, Tinloy B, et al. Cellular defects in Chediak-Higashi syndrome correlate with the molecular genotype and clinical phenotype. J Invest Dermatol. 2007;127:2674-7 pubmed
  • immunocytochemistry; human; 1:200
Helip Wooley A, Westbroek W, Dorward H, Koshoffer A, Huizing M, Boissy R, et al. Improper trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5. J Invest Dermatol. 2007;127:1471-8 pubmed
  • immunohistochemistry; human
Pecciarini L, Cangi M, Lo Cunsolo C, Macri E, Dal Cin E, Martignoni G, et al. Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient. Genes Chromosomes Cancer. 2007;46:419-26 pubmed
  • immunocytochemistry; mouse
Setty S, Tenza D, Truschel S, Chou E, Sviderskaya E, Theos A, et al. BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles. Mol Biol Cell. 2007;18:768-80 pubmed
  • immunocytochemistry; human
Theos A, Berson J, Theos S, Herman K, Harper D, Tenza D, et al. Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17. Mol Biol Cell. 2006;17:3598-612 pubmed
  • immunohistochemistry; human
Wankowicz Kalinska A, Mailliard R, Olson K, Graham F, Edington H, Kirkwood J, et al. Accumulation of low-avidity anti-melanocortin receptor 1 (anti-MC1R) CD8+ T cells in the lesional skin of a patient with melanoma-related depigmentation. Melanoma Res. 2006;16:165-74 pubmed
Zhang J, Ye Z, Br xe4 utigam L, Chakraborty P, Luo Z, Culpepper J, et al. A role for microsomal glutathione transferase 1 in melanin biosynthesis and melanoma progression. J Biol Chem. 2023;299:104920 pubmed publisher
Wang T, Xie S, Luo R, Shi L, Bai P, Wang X, et al. Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus. Cancer Biol Ther. 2020;21:4-11 pubmed publisher
Li Z, Wang C, Deng H, Wu J, Huang H, Sun R, et al. Robust Photodynamic Therapy Using 5-ALA-Incorporated Nanocomplexes Cures Metastatic Melanoma through Priming of CD4+CD8+ Double Positive T Cells. Adv Sci (Weinh). 2019;6:1802057 pubmed publisher
Ozaki Y, Shindoh J, Miura Y, Nakajima H, Oki R, Uchiyama M, et al. Serial pseudoprogression of metastatic malignant melanoma in a patient treated with nivolumab: a case report. BMC Cancer. 2017;17:778 pubmed publisher
Yu W, Wang Y, Jiang Y, Zhang W, Li Y. Distinct immunophenotypes and prognostic factors in renal cell carcinoma with sarcomatoid differentiation: a systematic study of 19 immunohistochemical markers in 42 cases. BMC Cancer. 2017;17:293 pubmed publisher
Dennis M, Delevoye C, Acosta Ruiz A, Hurbain I, Romao M, Hesketh G, et al. BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers. J Cell Biol. 2016;214:293-308 pubmed publisher
Liu G, Ying H, Zeng G, Wheeler C, Black K, Yu J. HER-2, gp100, and MAGE-1 are expressed in human glioblastoma and recognized by cytotoxic T cells. Cancer Res. 2004;64:4980-6 pubmed
Ramakrishna V, Treml J, Vitale L, Connolly J, O Neill T, Smith P, et al. Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules. J Immunol. 2004;172:2845-52 pubmed
Nichols S, Harper D, Berson J, Marks M. A novel splice variant of Pmel17 expressed by human melanocytes and melanoma cells lacking some of the internal repeats. J Invest Dermatol. 2003;121:821-30 pubmed
Berson J, Theos A, Harper D, Tenza D, Raposo G, Marks M. Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis. J Cell Biol. 2003;161:521-33 pubmed
product information
Product Type :
Antibody
Product Name :
PMEL Monoclonal Antibody (HMB45)
Catalog # :
MA1-34759
Quantity :
500 uL
Price :
US 524.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human
Applications :
Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1:1,000
Species :
Human
Clone :
HMB45
Isotype :
IgG1
Storage :
4 C, do not freeze
Description :
Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.
Immunogen :
The extract of pigmented melanoma metastases from lymph nodes.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1:1,000
Aliases :
95 kDa melanocyte-specific secreted glycoprotein; 95kDa melanocyte-specific secreted glycoprotein; D10H12S53E; D12S53E; D12S53Eh; gp100; gp87; M-alpha; M-beta; ME20; ME20M; ME20-M; ME20-S; Melanocyte lineage specific antigen GP100; melanocyte protein mel 17; melanocyte protein PMEL; Melanocyte protein Pmel 17; melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; P26; PMEL; Pmel 17; PMEL17; premelanosome protein; Secreted melanoma-associated ME20 antigen; SI; SIL; SILV; silver homolog; silver locus protein; silver locus protein homolog; silver, mouse, homolog of
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA