Rb Monoclonal Antibody (1F8) | Invitrogen MA1-34070 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Rb Monoclonal Antibody (1F8)

catalog :

MA1-34070

quantity :

500µL

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F8

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, immunohistochemistry - paraffin section

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 2	Walline H, Carey T, Goudsmit C, Bellile E, D Souza G, Peterson L, et al. High-Risk HPV, Biomarkers, and Outcome in Matched Cohorts of Head and Neck Cancer Patients Positive and Negative for HIV. Mol Cancer Res. 2017;15:179-188 pubmed publisher
immunohistochemistry - paraffin section; human	DeMichele A, Clark A, Tan K, Heitjan D, Gramlich K, Gallagher M, et al. CDK 4/6 inhibitor palbociclib (PD0332991) in Rb+ advanced breast cancer: phase II activity, safety, and predictive biomarker assessment. Clin Cancer Res. 2015;21:995-1001 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Wang T, Goodman M, McGough R, Weiss K, Rao U. Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma. Int J Surg Pathol. 2014;22:589-99 pubmed publisher
immunohistochemistry; human; 1:200	Sahi H, Savola S, Sihto H, Koljonen V, Bohling T, Knuutila S. RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup. APMIS. 2014;122:1157-66 pubmed publisher
immunohistochemistry; human	Yanagawa N, Wang A, Kohler D, Santos G, Sykes J, Xu J, et al. Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients. Lung Cancer. 2013;79:215-20 pubmed publisher
immunohistochemistry; human; 1:50	Franchi A, Baroni G, Sardi I, Giunti L, Capanna R, Campanacci D. Dedifferentiated peripheral chondrosarcoma: a clinicopathologic, immunohistochemical, and molecular analysis of four cases. Virchows Arch. 2012;460:335-42 pubmed publisher
western blot; human	Donati G, Brighenti E, Vici M, Mazzini G, Trere D, Montanaro L, et al. Selective inhibition of rRNA transcription downregulates E2F-1: a new p53-independent mechanism linking cell growth to cell proliferation. J Cell Sci. 2011;124:3017-28 pubmed publisher
immunohistochemistry; human	Canzonieri V, Barzan L, Franchin G, Vaccher E, Talamini R, Sulfaro S, et al. Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas. J Cell Physiol. 2012;227:233-8 pubmed publisher
immunohistochemistry; human	Kaune K, Neumann C, Hallermann C, Haller F, Schön M, Middel P. Simultaneous aberrations of single CDKN2A network components and a high Rb phosphorylation status can differentiate subgroups of primary cutaneous B-cell lymphomas. Exp Dermatol. 2011;20:331-5 pubmed publisher
western blot; human; 1:200	Lee S, Liu S, Mitchell R, Slagle Webb B, Hong Y, Sheehan J, et al. HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A. Int J Cancer. 2011;129:2104-14 pubmed publisher
immunohistochemistry; human; 1:50	Panelos J, Beltrami G, Scoccianti G, Capanna R, Paglierani M, Pepi M, et al. Prognostic significance of the alterations of the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue. Ann Surg Oncol. 2011;18:566-71 pubmed publisher
western blot; human; fig 2	Lin Q, Chen D, Timchenko N, Medrano E. SKI promotes Smad3 linker phosphorylations associated with the tumor-promoting trait of TGFbeta. Cell Cycle. 2010;9:1684-9 pubmed
western blot; human	Rampias T, Boutati E, Pectasides E, Sasaki C, Kountourakis P, Weinberger P, et al. Activation of Wnt signaling pathway by human papillomavirus E6 and E7 oncogenes in HPV16-positive oropharyngeal squamous carcinoma cells. Mol Cancer Res. 2010;8:433-43 pubmed publisher
EMSA; human; fig 3 western blot; human; 2 ug/ml; fig 2	Chimploy K, Díaz G, Li Q, Carter O, Dashwood W, Mathews C, et al. E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin. Int J Cancer. 2009;125:2086-94 pubmed publisher
western blot; human; 1:2000	Rampias T, Sasaki C, Weinberger P, Psyrri A. E6 and e7 gene silencing and transformed phenotype of human papillomavirus 16-positive oropharyngeal cancer cells. J Natl Cancer Inst. 2009;101:412-23 pubmed publisher
immunohistochemistry; human; 1:400	Fernandez Ranvier G, Khanafshar E, Tacha D, Wong M, Kebebew E, Duh Q, et al. Defining a molecular phenotype for benign and malignant parathyroid tumors. Cancer. 2009;115:334-44 pubmed publisher
western blot; human	Hori M, Suzuki K, Udono M, Yamauchi M, Mine M, Watanabe M, et al. Establishment of ponasterone A-inducible the wild-type p53 protein-expressing clones from HSC-1 cells, cell growth suppression by p53 expression and the suppression mechanism. Arch Dermatol Res. 2009;301:631-46 pubmed publisher
immunocytochemistry; human; 1:200 western blot; human; 1:200	Montanaro L, Mazzini G, Barbieri S, Vici M, Nardi Pantoli A, Govoni M, et al. Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines. Cell Prolif. 2007;40:532-49 pubmed
immunocytochemistry; human western blot; human	Bandyopadhyay D, Curry J, Lin Q, Richards H, Chen D, Hornsby P, et al. Dynamic assembly of chromatin complexes during cellular senescence: implications for the growth arrest of human melanocytic nevi. Aging Cell. 2007;6:577-91 pubmed
western blot; mouse	Kim J, Hong S, Lee B, Hong J, Kwak J, Cho S, et al. The inhibition of T-cells proliferation by mouse mesenchymal stem cells through the induction of p16INK4A-cyclin D1/cdk4 and p21waf1, p27kip1-cyclin E/cdk2 pathways. Cell Immunol. 2007;245:16-23 pubmed
immunohistochemistry; human; 1:200	Lee M, Tseng R, Hsu H, Chen J, Tzao C, Ho W, et al. Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer. Clin Cancer Res. 2007;13:832-8 pubmed
western blot; human	Choi J, Murillo G, Su B, Pezzuto J, Kinghorn A, Mehta R. Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells. FEBS J. 2006;273:5714-23 pubmed
immunohistochemistry; human	Nam E, Kim J, Kim S, Kim Y, Kim J, Yoon B, et al. The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance. Gynecol Oncol. 2007;104:207-11 pubmed
immunohistochemistry; human; 1:25	Yurakh A, Ramos D, Calabuig Fariñas S, Lopez Guerrero J, Rubio J, Solsona E, et al. Molecular and immunohistochemical analysis of the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder. Eur Urol. 2006;50:506-15; discussion 515 pubmed
western blot; rat	Kuo H, Kuo W, Lee Y, Lin W, Chou F, Tseng T. Inhibitory effect of caffeic acid phenethyl ester on the growth of C6 glioma cells in vitro and in vivo. Cancer Lett. 2006;234:199-208 pubmed
	Tzai T, Tsai Y, Chow N. The prevalence and clinicopathologic correlate of p16INK4a, retinoblastoma and p53 immunoreactivity in locally advanced urinary bladder cancer. Urol Oncol. 2004;22:112-8 pubmed
	Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari K, Abraham T, et al. p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma. Int J Cancer. 2004;109:710-6 pubmed
	Gorbunova V, Seluanov A, Pereira Smith O. Evidence that high telomerase activity may induce a senescent-like growth arrest in human fibroblasts. J Biol Chem. 2003;278:7692-8 pubmed
	Hennigan R, Stambrook P. Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes. Mol Biol Cell. 2001;12:2352-63 pubmed
	Jung C, Motwani M, Schwartz G. Flavopiridol increases sensitization to gemcitabine in human gastrointestinal cancer cell lines and correlates with down-regulation of ribonucleotide reductase M2 subunit. Clin Cancer Res. 2001;7:2527-36 pubmed
	Esteller M, Cordon Cardo C, Corn P, Meltzer S, Pohar K, Watkins D, et al. p14ARF silencing by promoter hypermethylation mediates abnormal intracellular localization of MDM2. Cancer Res. 2001;61:2816-21 pubmed
	Huschtscha L, Noble J, Neumann A, Moy E, Barry P, Melki J, et al. Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells. Cancer Res. 1998;58:3508-12 pubmed

product information

Product Type :

Antibody

Product Name :

Rb Monoclonal Antibody (1F8)

Catalog # :

MA1-34070

Quantity :

500µL

Price :

US 390

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): Assay dependent, Immunoprecipitation: Assay dependent, Western Blot: 1:100-1:500

Species :

Human, Mouse, Rat

Clone :

1F8

Isotype :

IgG1

Storage :

4° C, do not freeze

Description :

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Immunogen :

Recombinant full length protein (Human).

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): Assay dependent, Immunoprecipitation: Assay dependent, Western Blot: 1:100-1:500

Aliases :

OSRC, RB, p105-Rb, pRb, pp110