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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Estrogen Receptor alpha Monoclonal Antibody (33)
catalog :
MA1-310
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
33
reactivity :
human, mouse, rat, bovine, pigs , domestic sheep
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 48
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:30; loading ...; fig 2b
Mu X, Tu Z, Chen X, Hong Y, Geng Y, Zhang Y, et al. In utero Exposure to Excessive Estrogen Impairs Homologous Recombination and Oogenesis via Estrogen Receptor 2 in Mice. Front Cell Dev Biol. 2021;9:669732 pubmed publisher
  • immunohistochemistry - paraffin section; rat; loading ...; fig 6a
Ibrahim M, Elwan W. Role of topical dehydroepiandrosterone in ameliorating isotretinoin-induced Meibomian gland dysfunction in adult male albino rat. Ann Anat. 2017;211:78-87 pubmed publisher
  • immunocytochemistry; mouse; 1:50; fig 5
Ávila Rodriguez M, Garcia Segura L, Hidalgo Lanussa O, Baez E, Gonzalez J, Barreto G. Tibolone protects astrocytic cells from glucose deprivation through a mechanism involving estrogen receptor beta and the upregulation of neuroglobin expression. Mol Cell Endocrinol. 2016;433:35-46 pubmed publisher
  • flow cytometry; human; 1:20
Nguyen A, Yoshida M, Goodarzi H, Tavazoie S. Highly variable cancer subpopulations that exhibit enhanced transcriptome variability and metastatic fitness. Nat Commun. 2016;7:11246 pubmed publisher
  • western blot; pigs ; fig 3
Tran Q, Firkins R, Giles J, Francis S, Matnishian V, Tran P, et al. Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem. 2016;291:10805-23 pubmed publisher
  • western blot; human
Zhao H, Agazie Y. Inhibition of SHP2 in basal-like and triple-negative breast cells induces basal-to-luminal transition, hormone dependency, and sensitivity to anti-hormone treatment. BMC Cancer. 2015;15:109 pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; mouse; 1:200
Tannour Louet M, Han S, Louet J, Zhang B, Romero K, Addai J, et al. Increased gene copy number of VAMP7 disrupts human male urogenital development through altered estrogen action. Nat Med. 2014;20:715-24 pubmed publisher
  • western blot; human; 1:200
Lidke A, Bannister S, Löwer A, Apel D, Podleschny M, Kollmann M, et al. 17?-Estradiol induces supernumerary primordial germ cells in embryos of the polychaete Platynereis dumerilii. Gen Comp Endocrinol. 2014;196:52-61 pubmed publisher
  • EMSA; human
Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed publisher
  • western blot; mouse
Kalthoff S, Winkler A, Freiberg N, Manns M, Strassburg C. Gender matters: estrogen receptor alpha (ER?) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A). J Hepatol. 2013;59:797-804 pubmed publisher
  • immunohistochemistry; rat
Cetinkaya Demir B, Uyar Y, Ozbilgin K, Kose C. Effect of raloxifene and atorvastatin in atherosclerotic process in ovariectomized rats. J Obstet Gynaecol Res. 2013;39:229-36 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Endler A, Chen L, Zhang J, Xu G, Shibasaki F. Binding of the ER? and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is essential for estrogen- and dioxin-related transcription. J Cell Sci. 2012;125:2004-16 pubmed publisher
  • immunohistochemistry; human; 1:100
Jenei Lanzl Z, Straub R, Dienstknecht T, Huber M, Hager M, Grassel S, et al. Estradiol inhibits chondrogenic differentiation of mesenchymal stem cells via nonclassic signaling. Arthritis Rheum. 2010;62:1088-96 pubmed publisher
  • immunohistochemistry; human; 1:200
  • western blot; mouse; 1:500
Killer N, Hock M, Gehlhaus M, Capetian P, Knoth R, Pantazis G, et al. Modulation of androgen and estrogen receptor expression by antiepileptic drugs and steroids in hippocampus of patients with temporal lobe epilepsy. Epilepsia. 2009;50:1875-90 pubmed publisher
  • immunohistochemistry; domestic sheep; 1:50
  • western blot; domestic sheep; 1:200
Ortega H, Salvetti N, Padmanabhan V. Developmental programming: prenatal androgen excess disrupts ovarian steroid receptor balance. Reproduction. 2009;137:865-77 pubmed publisher
  • immunohistochemistry; rat; 1:100
Zhao Q, Tan Z, Guo J, Chen Y. Estrous cycle-dependent expression of estrogen receptor alpha in periodontal tissue. Chronobiol Int. 2007;24:425-33 pubmed
  • western blot; mouse
Köhler H, Kloas W, Schirling M, Lutz I, Reye A, Langen J, et al. Sex steroid receptor evolution and signalling in aquatic invertebrates. Ecotoxicology. 2007;16:131-43 pubmed
  • immunohistochemistry; human
Capellino S, Riepl B, Rauch L, Angele P, Cutolo M, Straub R. Quantitative determination of steroid hormone receptor positive cells in the synovium of patients with rheumatoid arthritis and osteoarthritis: is there a link to inflammation?. Ann Rheum Dis. 2007;66:53-8 pubmed
  • flow cytometry; bovine
Lamote I, Meyer E, De Ketelaere A, Duchateau L, Burvenich C. Expression of the estrogen receptor in blood neutrophils of dairy cows during the periparturient period. Theriogenology. 2006;65:1082-98 pubmed
Corpuz Hilsabeck M, Mohajer N, Culty M. Dysregulation of Immature Sertoli Cell Functions by Exposure to Acetaminophen and Genistein in Rodent Cell Models. Cells. 2023;12: pubmed publisher
Li Z, Chen L, Zhang D, Huang X, Yang J, Li W, et al. Intranasal 15d-PGJ2 inhibits the growth of rat lactotroph pituitary neuroendocrine tumors by inducing PPARγ-dependent apoptotic and autophagic cell death. Front Neurosci. 2023;17:1109675 pubmed publisher
Kata D, Gr xf3 f I, Hoyk Z, Ducza E, Deli M, Zupk xf3 I, et al. Immunofluorescent Evidence for Nuclear Localization of Aromatase in Astrocytes in the Rat Central Nervous System. Int J Mol Sci. 2022;23: pubmed publisher
Kotlarczyk A, Grzyb M, Korzekwa A. Regulation of uterine function during estrous cycle, anestrus phase and pregnancy by steroids in red deer (Cervus elaphus L.). Sci Rep. 2021;11:20109 pubmed publisher
Sunny D, Hammer E, Ittermann T, Kr xfc ger E, H xfc bner S, Hartmann M, et al. Fetal Zone Steroids and Estrogen Show Sex Specific Effects on Oligodendrocyte Precursor Cells in Response to Oxidative Damage. Int J Mol Sci. 2021;22: pubmed publisher
Barrière D, Midavaine E, Doré Savard L, Kirby K, Tremblay L, Beaudoin J, et al. Dichotomic effects of clinically used drugs on tumor growth, bone remodeling and pain management. Sci Rep. 2019;9:20155 pubmed publisher
Ambhore N, Kalidhindi R, Pabelick C, Hawse J, Prakash Y, Sathish V. Differential estrogen-receptor activation regulates extracellular matrix deposition in human airway smooth muscle remodeling via NF-κB pathway. FASEB J. 2019;33:13935-13950 pubmed publisher
Ambhore N, Katragadda R, Raju Kalidhindi R, Thompson M, Pabelick C, Prakash Y, et al. Estrogen receptor beta signaling inhibits PDGF induced human airway smooth muscle proliferation. Mol Cell Endocrinol. 2018;476:37-47 pubmed publisher
Dhawan L, Liu B, Blaxall B, Taubman M. A novel role for the glucocorticoid receptor in the regulation of monocyte chemoattractant protein-1 mRNA stability. J Biol Chem. 2007;282:10146-52 pubmed
Tilton S, Foran C, Benson W. Effects of cadmium on the reproductive axis of Japanese medaka (Oryzias latipes). Comp Biochem Physiol C Toxicol Pharmacol. 2003;136:265-76 pubmed
Kobayashi K, Iwakiri R, Kobayashi H, Okinami S. Expression of estrogen receptor alpha and 17beta-hydroxysteroid dehydrogenase 4 in the ciliary body. Graefes Arch Clin Exp Ophthalmol. 2004;242:172-176 pubmed publisher
Gopalkrishnan K, Katkam R, Sachdeva G, Kholkute S, Padwal V, Puri C. Effects of an antiprogestin onapristone on the endometrium of bonnet monkeys: morphometric and ultrastructural studies. Biol Reprod. 2003;68:1959-67 pubmed
Wiren K, Chapman Evans A, Zhang X. Osteoblast differentiation influences androgen and estrogen receptor-alpha and -beta expression. J Endocrinol. 2002;175:683-94 pubmed
Kobayashi K, Mandai M, Suzuma I, Kobayashi H, Okinami S. Expression of estrogen receptor in the choroidal neovascular membranes in highly myopic eyes. Retina. 2002;22:418-22 pubmed
Foran C, Peterson B, Benson W. Transgenerational and developmental exposure of Japanese medaka (Oryzias latipes) to ethinylestradiol results in endocrine and reproductive differences in the response to ethinylestradiol as adults. Toxicol Sci. 2002;68:389-402 pubmed
van der Eerden B, Gevers E, Lowik C, Karperien M, Wit J. Expression of estrogen receptor alpha and beta in the epiphyseal plate of the rat. Bone. 2002;30:478-85 pubmed
Song R, McPherson R, Adam L, Bao Y, Shupnik M, Kumar R, et al. Linkage of rapid estrogen action to MAPK activation by ERalpha-Shc association and Shc pathway activation. Mol Endocrinol. 2002;16:116-27 pubmed
Cao S, Hudnall S, Kohen F, Lu L. Measurement of estrogen receptors in intact cells by flow cytometry. Cytometry. 2000;41:109-14 pubmed
Mendez M, Chavez B, Echeverria O, Vilchis F, Vázquez Nin G, Pedernera E. Evidence for estrogen receptor expression in germ cell and somatic cell subpopulations in the ovary of the newly hatched chicken. Cell Tissue Res. 1999;298:145-52 pubmed
Suzuma I, Mandai M, Takagi H, Suzuma K, Otani A, Oh H, et al. 17 Beta-estradiol increases VEGF receptor-2 and promotes DNA synthesis in retinal microvascular endothelial cells. Invest Ophthalmol Vis Sci. 1999;40:2122-9 pubmed
Falkner K, Xiao G, Pinaire J, Pendleton M, Lindahl R, Prough R. The negative regulation of the rat aldehyde dehydrogenase 3 gene by glucocorticoids: involvement of a single imperfect palindromic glucocorticoid responsive element. Mol Pharmacol. 1999;55:649-57 pubmed
Kobayashi K, Kobayashi H, Ueda M, Honda Y. Estrogen receptor expression in bovine and rat retinas. Invest Ophthalmol Vis Sci. 1998;39:2105-10 pubmed
Yang N, Venugopalan M, Hardikar S, Glasebrook A. Identification of an estrogen response element activated by metabolites of 17beta-estradiol and raloxifene. Science. 1996;273:1222-5 pubmed
Shevde N, Pike J. Estrogen modulates the recruitment of myelopoietic cell progenitors in rat through a stromal cell-independent mechanism involving apoptosis. Blood. 1996;87:2683-92 pubmed
Ikegami A, Inoue S, Hosoi T, Mizuno Y, Nakamura T, Ouchi Y, et al. Immunohistochemical detection and northern blot analysis of estrogen receptor in osteoblastic cells. J Bone Miner Res. 1993;8:1103-9 pubmed
Sierralta W, Bonig I, Thole H. Immunogold labelling of estradiol receptor in MCF7 cells. Cell Tissue Res. 1995;279:445-52 pubmed
Orimo A, Inoue S, Ikegami A, Hosoi T, Akishita M, Ouchi Y, et al. Vascular smooth muscle cells as target for estrogen. Biochem Biophys Res Commun. 1993;195:730-6 pubmed
Traish A, Ettinger R, Kim N, Marshak Rothstein A, Wotiz H. Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor. Steroids. 1990;55:196-208 pubmed
Watanabe M, Tanaka H, Kamada M, Okano J, Takahashi H, Uchida K, et al. Establishment of the human BSMZ breast cancer cell line, which overexpresses the erbB-2 and c-myc genes. Cancer Res. 1992;52:5178-82 pubmed
product information
Product Type :
Antibody
Product Name :
Estrogen Receptor alpha Monoclonal Antibody (33)
Catalog # :
MA1-310
Quantity :
100 ug
Price :
US 471.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Bovine, Human, Mouse, Non-human primate, Rat
Applications :
Immunocytochemistry: 10 ug/mL, Immunohistochemistry: 1:20 - 1:100, Western Blot: 4 ug/mL
Species :
Bovine, Human, Mouse, Non-human primate, Rat
Clone :
33
Isotype :
IgG1
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Immunogen :
Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 10 ug/mL, Immunohistochemistry: 1:20 - 1:100, Western Blot: 4 ug/mL
Aliases :
DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA