Estrogen Receptor alpha Monoclonal Antibody (6F11) | Invitrogen MA1-27107 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Estrogen Receptor alpha Monoclonal Antibody (6F11)

catalog :

MA1-27107

quantity :

250 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F11

reactivity :

human, mouse, rat, pigs , cat, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, EMSA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; domestic rabbit; 1:200; loading ...; fig 4a western blot; domestic rabbit; 1:200; loading ...; fig 4d	Brooks S, Dykes A, Schreurs B. A High-Cholesterol Diet Increases 27-Hydroxycholesterol and Modifies Estrogen Receptor Expression and Neurodegeneration in Rabbit Hippocampus. J Alzheimers Dis. 2017;56:185-196 pubmed publisher
immunohistochemistry - paraffin section; cat; 1:125; loading ...; tbl 4	Soares M, Ribeiro R, Najmudin S, Gameiro A, Rodrigues R, Cardoso F, et al. Serum HER2 levels are increased in cats with mammary carcinomas and predict tissue HER2 status. Oncotarget. 2016;7:17314-26 pubmed publisher
immunohistochemistry; cat; 1:100; loading ...; fig 1a	Soares M, Correia J, Peleteiro M, Ferreira F. St Gallen molecular subtypes in feline mammary carcinoma and paired metastases-disease progression and clinical implications from a 3-year follow-up study. Tumour Biol. 2016;37:4053-64 pubmed publisher
immunohistochemistry; pigs ; fig s5	Rowson Hodel A, Manjarin R, Trott J, Cardiff R, Borowsky A, Hovey R. Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo. BMC Cancer. 2015;15:562 pubmed publisher
immunocytochemistry; rat	Tkalia I, Vorobyova L, Grabovoy A, Svintsitsky V, Tarasova T. The antitumor efficacy of cisplatin in combination with triptorelin and exemestane therapy for an ovarian cancer ascites model in Wistar rats. Exp Oncol. 2015;37:30-5 pubmed
immunohistochemistry; human immunohistochemistry; rat	Tkalia I, Vorobyova L, Grabovoy A, Svintsitsky V, Tarasova T, Lukyanova N, et al. Increase of antitumor activity of cisplatin using agonist of gonadotropin-realising hormone and inhibitor of aromatase on the model of ascites ovarian tumor. Exp Oncol. 2014;36:184-90 pubmed
immunohistochemistry; human; 1:200	Khandakar B, Mathur S, Kumar L, Kumar S, Datta Gupta S, Iyer V, et al. Tissue biomarkers in prognostication of serous ovarian cancer following neoadjuvant chemotherapy. Biomed Res Int. 2014;2014:401245 pubmed publisher
western blot; human	Lee J, Tiwari A, Shum V, Mills G, Mancini M, Igoshin O, et al. Unraveling the regulatory connections between two controllers of breast cancer cell fate. Nucleic Acids Res. 2014;42:6839-49 pubmed publisher
immunohistochemistry; human; 1:100	Bredow L, Stützel L, Böhringer D, Gundlach E, Reinhard T, Auw Haedrich C. Progesterone and estrogen receptors in conjunctival melanoma and nevi. Graefes Arch Clin Exp Ophthalmol. 2014;252:359-65 pubmed publisher
EMSA; human	Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed publisher
immunohistochemistry; human; 1:50	Grosso S, Katayama M, Roela R, Nonogaki S, Soares F, Brentani H, et al. Breast cancer tissue slices as a model for evaluation of response to rapamycin. Cell Tissue Res. 2013;352:671-84 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 1	Corrêa N, Kuasne H, Faria J, Seixas C, Santos I, Abreu F, et al. Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors. Oncol Rep. 2013;29:1299-307 pubmed publisher
immunohistochemistry; human western blot; human	Li Y, Meeran S, Patel S, Chen H, Hardy T, Tollefsbol T. Epigenetic reactivation of estrogen receptor-Î± (ERÎ±) by genistein enhances hormonal therapy sensitivity in ERÎ±-negative breast cancer. Mol Cancer. 2013;12:9 pubmed publisher
immunohistochemistry; human; 1:50	Martins F, De S, Almendro V, Gonen M, Park S, Blum J, et al. Evolutionary pathways in BRCA1-associated breast tumors. Cancer Discov. 2012;2:503-11 pubmed publisher
immunohistochemistry; human; 1:100	Hovanessian Larsen L, Taylor D, Hawes D, Spicer D, Press M, Wu A, et al. Lowering oral contraceptive norethindrone dose increases estrogen and progesterone receptor levels with no reduction in proliferation of breast epithelium: a randomized trial. Contraception. 2012;86:238-43 pubmed publisher
immunohistochemistry; human; 1:100	Chung K, Hovanessian Larsen L, Hawes D, Taylor D, Downey S, Spicer D, et al. Breast epithelial cell proliferation is markedly increased with short-term high levels of endogenous estrogen secondary to controlled ovarian hyperstimulation. Breast Cancer Res Treat. 2012;132:653-60 pubmed publisher
immunohistochemistry; human; 1:50	Logullo A, Stiepcich M, Osório C, Nonogaki S, Pasini F, Rocha R, et al. Role of Fos-related antigen 1 in the progression and prognosis of ductal breast carcinoma. Histopathology. 2011;58:617-25 pubmed publisher
immunohistochemistry; rat; 1:100	WIEHLE R, Lantvit D, Yamada T, Christov K. CDB-4124, a progesterone receptor modulator, inhibits mammary carcinogenesis by suppressing cell proliferation and inducing apoptosis. Cancer Prev Res (Phila). 2011;4:414-24 pubmed publisher
immunohistochemistry; human; 1:50	Joosse S, Brandwijk K, Mulder L, Wesseling J, Hannemann J, Nederlof P. Genomic signature of BRCA1 deficiency in sporadic basal-like breast tumors. Genes Chromosomes Cancer. 2011;50:71-81 pubmed publisher
immunohistochemistry; human; 1:50	Joosse S, Brandwijk K, Devilee P, Wesseling J, Hogervorst F, Verhoef S, et al. Prediction of BRCA2-association in hereditary breast carcinomas using array-CGH. Breast Cancer Res Treat. 2012;132:379-89 pubmed publisher
immunohistochemistry; human; 1:100	Niwinska A, Murawska M, Pogoda K. Breast cancer subtypes and response to systemic treatment after whole-brain radiotherapy in patients with brain metastases. Cancer. 2010;116:4238-47 pubmed publisher
immunohistochemistry; rat; 1:250 western blot; rat; 1:1000	Puri J, Hutchins B, Bellinger L, Kramer P. Estrogen and inflammation modulate estrogen receptor alpha expression in specific tissues of the temporomandibular joint. Reprod Biol Endocrinol. 2009;7:155 pubmed publisher
immunohistochemistry; human; 1:50	Roepman P, Horlings H, Krijgsman O, Kok M, Bueno de Mesquita J, Bender R, et al. Microarray-based determination of estrogen receptor, progesterone receptor, and HER2 receptor status in breast cancer. Clin Cancer Res. 2009;15:7003-11 pubmed publisher
immunohistochemistry; human	Taylor D, Pearce C, Hovanessian Larsen L, Downey S, Spicer D, Bartow S, et al. Progesterone and estrogen receptors in pregnant and premenopausal non-pregnant normal human breast. Breast Cancer Res Treat. 2009;118:161-8 pubmed publisher
immunohistochemistry; human; 1:50	Horlings H, van Laar R, Kerst J, Helgason H, Wesseling J, van Der Hoeven J, et al. Gene expression profiling to identify the histogenetic origin of metastatic adenocarcinomas of unknown primary. J Clin Oncol. 2008;26:4435-41 pubmed publisher
immunohistochemistry - frozen section; human; 1:100 immunohistochemistry; human; 1:100	Shi S, Liu C, Pootrakul L, Tang L, Young A, Chen R, et al. Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry. Am J Clin Pathol. 2008;129:358-66 pubmed publisher
immunohistochemistry; human	Barbareschi M, Buttitta F, Felicioni L, Cotrupi S, Barassi F, Del Grammastro M, et al. Different prognostic roles of mutations in the helical and kinase domains of the PIK3CA gene in breast carcinomas. Clin Cancer Res. 2007;13:6064-9 pubmed
immunohistochemistry; domestic rabbit	Lin A, Levin R, Kogan B, Whitbeck C, Chichester P, Sokol R, et al. Estrogen induced functional hypertrophy and increased force generation of the female rabbit bladder. Neurourol Urodyn. 2006;25:473-9 pubmed
immunohistochemistry; rat western blot; rat	Weroha S, Li S, Tawfik O, Li J. Overexpression of cyclins D1 and D3 during estrogen-induced breast oncogenesis in female ACI rats. Carcinogenesis. 2006;27:491-8 pubmed
immunohistochemistry; human	Inceboz U, Nese N, Uyar Y, Ozcakir H, Kurtul O, Baytur Y, et al. Hormone receptor expressions and proliferation markers in postmenopausal endometrial polyps. Gynecol Obstet Invest. 2006;61:24-8 pubmed
immunohistochemistry; human; 1:100	Akhan S, Yavuz E, Tecer A, Iyibozkurt C, Topuz S, Tuzlali S, et al. The expression of Ki-67, p53, estrogen and progesterone receptors affecting survival in uterine leiomyosarcomas. A clinicopathologic study. Gynecol Oncol. 2005;99:36-42 pubmed
immunohistochemistry - paraffin section; human; 1:80; fig 6	Kuo K, Chen M, Lin M. Epithelioid trophoblastic tumor of the broad ligament: a case report and review of the literature. Am J Surg Pathol. 2004;28:405-9 pubmed
immunohistochemistry - paraffin section; rat; tbl 1	Goyal H, Braden T, Williams C, Dalvi P, Mansour M, Mansour M, et al. Abnormal morphology of the penis in male rats exposed neonatally to diethylstilbestrol is associated with altered profile of estrogen receptor-alpha protein, but not of androgen receptor protein: a developmental and immunocytochemical study. Biol Reprod. 2004;70:1504-17 pubmed
immunohistochemistry - paraffin section; human; tbl 2	Halperin R, Zehavi S, Hadas E, Habler L, Bukovsky I, Schneider D. Simultaneous carcinoma of the endometrium and ovary vs endometrial carcinoma with ovarian metastases: a clinical and immunohistochemical determination. Int J Gynecol Cancer. 2003;13:32-7 pubmed
immunohistochemistry - paraffin section; human; tbl 1	Herbert M, Sandbank J, Liokumovich P, Yanai O, Pappo I, Karni T, et al. Breast hamartomas: clinicopathological and immunohistochemical studies of 24 cases. Histopathology. 2002;41:30-4 pubmed
immunohistochemistry - paraffin section; human; fig 1	Halperin R, Zehavi S, Hadas E, Habler L, Bukovsky I, Schneider D. Immunohistochemical comparison of primary peritoneal and primary ovarian serous papillary carcinoma. Int J Gynecol Pathol. 2001;20:341-5 pubmed
	Kessel J, Weiskirchen R, Schr xf6 der S. Expression Analysis of Lipocalin 2 (LCN2) in Reproductive and Non-Reproductive Tissues of Esr1-Deficient Mice. Int J Mol Sci. 2023;24: pubmed publisher
	Kapucuoglu N, Coban T, Raunio H, Pelkonen O, Edwards R, Boobis A, et al. Expression of CYP3A4 in human breast tumour and non-tumour tissues. Cancer Lett. 2003;202:17-23 pubmed
	Beliakoff J, Bagatell R, Paine Murrieta G, Taylor C, Lykkesfeldt A, Whitesell L. Hormone-refractory breast cancer remains sensitive to the antitumor activity of heat shock protein 90 inhibitors. Clin Cancer Res. 2003;9:4961-71 pubmed
	Zinser G, McEleney K, Welsh J. Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice. Mol Cell Endocrinol. 2003;200:67-80 pubmed
	Querzoli P, Albonico G, di Iasio M, Ferretti S, Rinaldi R, Cariello A, et al. Biophenotypes and survival of BRCA1 and TP53 deleted breast cancer in young women. Breast Cancer Res Treat. 2001;66:135-42 pubmed
	Zunarelli E, Nicoll J, Migaldi M, Trentini G. Apolipoprotein E polymorphism and breast carcinoma: correlation with cell proliferation indices and clinical outcome. Breast Cancer Res Treat. 2000;63:193-8 pubmed

product information

Product Type :

Antibody

Product Name :

Estrogen Receptor alpha Monoclonal Antibody (6F11)

Catalog # :

MA1-27107

Quantity :

250 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ChIP Assay: 5 uL/10^6 cells, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:40-1:60, Immunohistochemistry (Paraffin): 1:40-1:80, Western Blot: 1:50

Species :

Human, Mouse, Rat

Clone :

6F11

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

Immunogen :

Recombinant estrogen receptor protein (alpha form)

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 5 uL/10^6 cells, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:40-1:60, Immunohistochemistry (Paraffin): 1:40-1:80, Western Blot: 1:50

Aliases :

DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product

more info or order :

Invitrogen product webpage