product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
P-Glycoprotein Monoclonal Antibody (C219)
catalog :
MA1-26528
quantity :
250 uL
price :
US 546.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C219
reactivity :
Chinese hamsters, human, mouse, rat, dogs, bovine, pigs , fruit fly
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation
more info or order :
Invitrogen product webpage
citations: 39
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; loading ...
  • western blot knockout validation; mouse; loading ...
Vita S, Redell J, Maynard M, Zhao J, Grill R, Dash P, et al. P-glycoprotein Expression Is Upregulated in a Pre-Clinical Model of Traumatic Brain Injury. Neurotrauma Rep. 2020;1:207-217 pubmed publisher
  • western blot; mouse; 1 ug/ml; fig 1c
Lin A, Parikh I, Yanckello L, White R, Hartz A, Taylor C, et al. APOE genotype-dependent pharmacogenetic responses to rapamycin for preventing Alzheimer's disease. Neurobiol Dis. 2020;139:104834 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:20; loading ...; fig s4
Weidner L, Kannan P, Mitsios N, Kang S, Hall M, Theodore W, et al. The expression of inflammatory markers and their potential influence on efflux transporters in drug-resistant mesial temporal lobe epilepsy tissue. Epilepsia. 2018;59:1507-1517 pubmed publisher
  • immunohistochemistry; fruit fly ; fig s2d
Zhang S, Yue Z, Arnold D, Artiushin G, Sehgal A. A Circadian Clock in the Blood-Brain Barrier Regulates Xenobiotic Efflux. Cell. 2018;173:130-139.e10 pubmed publisher
  • immunohistochemistry - frozen section; rat; fig 2c
  • western blot; rat; 1:75; fig 2b
Sun X, Duan Z, Liu Z, Tang S, Li Y, He S, et al. Inhibition of P-glycoprotein, multidrug resistance-associated protein 2 and cytochrome P450 3A4 improves the oral absorption of octreotide in rats with portal hypertension. Exp Ther Med. 2016;12:3716-3722 pubmed publisher
  • western blot; mouse; fig 1A
Esser L, Zhou F, Pluchino K, Shiloach J, Ma J, Tang W, et al. Structures of the Multidrug Transporter P-glycoprotein Reveal Asymmetric ATP Binding and the Mechanism of Polyspecificity. J Biol Chem. 2017;292:446-461 pubmed publisher
  • western blot; mouse; 1 ug/ml; loading ...; fig 2a
Parikh I, Guo J, Chuang K, Zhong Y, Rempe R, Hoffman J, et al. Caloric restriction preserves memory and reduces anxiety of aging mice with early enhancement of neurovascular functions. Aging (Albany NY). 2016;8:2814-2826 pubmed publisher
  • western blot; pigs ; 1:1000
Hasibu I, Patoine D, Pilote S, Drolet B, Simard C. The guinea-pig expresses functional CYP2C and P-glycoprotein: further validation of its usefulness in drug biotransformation/transport studies. Biopharm Drug Dispos. 2015;36:183-203 pubmed publisher
  • western blot; human; 1:20
Boyer Di Ponio J, El Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE. 2014;9:e84179 pubmed publisher
  • immunocytochemistry; dogs; 1:100
Georgantzopoulou A, Skoczyńska E, van den Berg J, Brand W, Legay S, Klein S, et al. P-gp efflux pump inhibition potential of common environmental contaminants determined in vitro. Environ Toxicol Chem. 2014;33:804-13 pubmed publisher
  • western blot; human
Katayama K, Yamaguchi M, Noguchi K, Sugimoto Y. Protein phosphatase complex PP5/PPP2R3C dephosphorylates P-glycoprotein/ABCB1 and down-regulates the expression and function. Cancer Lett. 2014;345:124-31 pubmed publisher
  • western blot; human; 1:50
Deng Z, Yan F, Jin Q, Li F, Wu J, Liu X, et al. Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome-microbubble complexes assisted by ultrasound. J Control Release. 2014;174:109-16 pubmed publisher
Slattery O, Dahle M, Sundaram A, Nowak B, Gjessing M, Solhaug A. Functional and molecular characterization of the Atlantic salmon gill epithelium cell line ASG-10; a tool for in vitro gill research. Front Mol Biosci. 2023;10:1242879 pubmed publisher
Vulin M, Zhong Y, Maloney B, Bauer B, Hartz A. Proteasome inhibition protects blood-brain barrier P-glycoprotein and lowers Aβ brain levels in an Alzheimer's disease model. Fluids Barriers CNS. 2023;20:70 pubmed publisher
Fuchs D, Rohrer Bley C, Morandi L, Tonon C, Weyland M, Nytko K. Triple combination of lomustine, temozolomide and irradiation reduces canine glioma cell survival in vitro. Vet Med Sci. 2023;9:1573-1583 pubmed publisher
Mo P, Chen H, Jiang X, Hu F, Zhang F, Shan G, et al. Effect of hepatic NPC1L1 on cholesterol gallstone disease and its mechanism. Heliyon. 2023;9:e15757 pubmed publisher
Liu N, Zhu Y, Yu K, Gu Z, Lv S, Chen Y, et al. Functional Blood-Brain Barrier Model with Tight Connected Minitissue by Liquid Substrates Culture. Adv Healthc Mater. 2023;12:e2201984 pubmed publisher
Nguyen V, Ye S, Gkouzioti V, van Wolferen M, Yengej F, Melkert D, et al. A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles. J Extracell Vesicles. 2022;11:e12280 pubmed publisher
Gericke B, Wienb xf6 ker I, Brandes G, L xf6 scher W. Is P-Glycoprotein Functionally Expressed in the Limiting Membrane of Endolysosomes? A Biochemical and Ultrastructural Study in the Rat Liver. Cells. 2022;11: pubmed publisher
Chen H, Deng S, Albadari N, Yun M, Zhang S, Li Y, et al. Design, Synthesis, and Biological Evaluation of Stable Colchicine-Binding Site Tubulin Inhibitors 6-Aryl-2-benzoyl-pyridines as Potential Anticancer Agents. J Med Chem. 2021;64:12049-12074 pubmed publisher
Lahmar A, Mathey A, Aires V, Elgueder D, Vejux A, Khlifi R, et al. Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum, Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein. Nutrients. 2021;13: pubmed publisher
Ding Y, Zhong Y, Baldeshwiler A, Abner E, Bauer B, Hartz A. Protecting P-glycoprotein at the blood-brain barrier from degradation in an Alzheimer's disease mouse model. Fluids Barriers CNS. 2021;18:10 pubmed publisher
Mahmud F, Deng S, Chen H, Miller D, Li W. Orally available tubulin inhibitor VERU-111 enhances antitumor efficacy in paclitaxel-resistant lung cancer. Cancer Lett. 2020;495:76-88 pubmed publisher
Kropf C, Fent K, Fischer S, Casanova A, Segner H. ABC transporters in gills of rainbow trout (Oncorhynchus mykiss). J Exp Biol. 2020;223: pubmed publisher
Shi Y, Sun L, Wang M, Liu J, Zhong S, Li R, et al. Vascularized human cortical organoids (vOrganoids) model cortical development in vivo. PLoS Biol. 2020;18:e3000705 pubmed publisher
Hartz A, Rempe R, Soldner E, Pekcec A, Schlichtiger J, Kryscio R, et al. Cytosolic phospholipase A2 is a key regulator of blood-brain barrier function in epilepsy. FASEB J. 2019;33:14281-14295 pubmed publisher
Deng S, Krutilina R, Wang Q, Lin Z, Parke D, Playa H, et al. An Orally Available Tubulin Inhibitor, VERU-111, Suppresses Triple-Negative Breast Cancer Tumor Growth and Metastasis and Bypasses Taxane Resistance. Mol Cancer Ther. 2020;19:348-363 pubmed publisher
Soldner E, Hartz A, Akanuma S, Pekcec A, Doods H, Kryscio R, et al. Inhibition of human microsomal PGE2 synthase-1 reduces seizure-induced increases of P-glycoprotein expression and activity at the blood-brain barrier. FASEB J. 2019;33:13966-13981 pubmed publisher
Fei Z, Hu M, Baum L, Kwan P, Hong T, Zhang C. The potential role of human multidrug resistance protein 1 (MDR1) and multidrug resistance-associated protein 2 (MRP2) in the transport of Huperzine A in vitro. Xenobiotica. 2020;50:354-362 pubmed publisher
Baglo Y, Liang B, Robey R, Ambudkar S, Gottesman M, Huang H. Porphyrin-lipid assemblies and nanovesicles overcome ABC transporter-mediated photodynamic therapy resistance in cancer cells. Cancer Lett. 2019;457:110-118 pubmed publisher
Hartz A, Zhong Y, Shen A, Abner E, Bauer B. Preventing P-gp Ubiquitination Lowers Aβ Brain Levels in an Alzheimer's Disease Mouse Model. Front Aging Neurosci. 2018;10:186 pubmed publisher
Ma D, Wang A, Parikh I, Green S, Hoffman J, Chlipala G, et al. Ketogenic diet enhances neurovascular function with altered gut microbiome in young healthy mice. Sci Rep. 2018;8:6670 pubmed publisher
Hoffman J, Parikh I, Green S, Chlipala G, Mohney R, KEATON M, et al. Age Drives Distortion of Brain Metabolic, Vascular and Cognitive Functions, and the Gut Microbiome. Front Aging Neurosci. 2017;9:298 pubmed publisher
Zhang Y, Dai C, Yuan C, Wu H, Xiao Z, Lei Z, et al. Establishment and characterization of arsenic trioxide resistant KB/ATO cells. Acta Pharm Sin B. 2017;7:564-570 pubmed publisher
Wang M, Mao C, Wang H, Ling X, Wu Z, Li Z, et al. Molecular Imaging of P-glycoprotein in Chemoresistant Tumors Using a Dual-Modality PET/Fluorescence Probe. Mol Pharm. 2017;14:3391-3398 pubmed publisher
Baek J, Cho C. A multifunctional lipid nanoparticle for co-delivery of paclitaxel and curcumin for targeted delivery and enhanced cytotoxicity in multidrug resistant breast cancer cells. Oncotarget. 2017;8:30369-30382 pubmed publisher
Chan G, Evans R, Banks D, Mesev E, Miller D, Cannon R. Selective induction of P-glycoprotein at the CNS barriers during symptomatic stage of an ALS animal model. Neurosci Lett. 2017;639:103-113 pubmed publisher
Hartz A, Zhong Y, Wolf A, LeVine H, Miller D, Bauer B. Aβ40 Reduces P-Glycoprotein at the Blood-Brain Barrier through the Ubiquitin-Proteasome Pathway. J Neurosci. 2016;36:1930-41 pubmed publisher
Willyerd F, Empey P, Philbrick A, Ikonomovic M, Puccio A, Kochanek P, et al. Expression of ATP-Binding Cassette Transporters B1 and C1 after Severe Traumatic Brain Injury in Humans. J Neurotrauma. 2016;33:226-31 pubmed publisher
product information
Product Type :
Antibody
Product Name :
P-Glycoprotein Monoclonal Antibody (C219)
Catalog # :
MA1-26528
Quantity :
250 uL
Price :
US 546.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Bovine, Fish, Hamster, Human, Mouse, Rat, Zebrafish
Applications :
ELISA: Assay-dependent, Flow Cytometry: 5-10 ug for 1x10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 5-10 ug/mL, Immunohistochemistry (Paraffin): 1:25, Immunoprecipitation: Assay-dependent, Western Blot: 1:50-1:600
Species :
Bovine, Fish, Hamster, Human, Mouse, Rat, Zebrafish
Clone :
C219
Isotype :
IgG1
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
Immunogen :
SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: 5-10 ug for 1x10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 5-10 ug/mL, Immunohistochemistry (Paraffin): 1:25, Immunoprecipitation: Assay-dependent, Western Blot: 1:50-1:600
Aliases :
ABC superfamily; P-glycoprotein; ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; ATP binding cassette subfamily B member 1; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette transporter subfamily B, member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; ATP-dependent translocase ABCB1; multidrug resistance protein 1; bovine p-glycoprotein; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; Evi32; GP170; mdr; Mdr1; MDR1A; Mdr1b; Mdr3; mdr-3; multi-drug resistance 3; multidrug resistance p-glycoprotein; multidrug resistance protein 1; Multidrug resistance protein 1A; multidrug resistance protein 1B; Multidrug resistance protein 2; multidrug resistance protein 3; multidrug resistance protein MDR1; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein; P-glycoprotein 1; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Pgp; p-gp; PGP1; PGP2; PGY1; Pgy-1; Pgy1-1; PGY2; Pgy3; Pgy-3; Phospholipid transporter ABCB1
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA