POLR2A Monoclonal Antibody (8WG16) | Invitrogen MA1-26249 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

POLR2A Monoclonal Antibody (8WG16)

catalog :

MA1-26249

quantity :

100 ug

price :

US 480.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8WG16

reactivity :

fission yeast, human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; fission yeast; loading ...; fig 3b	Ohle C, Tesorero R, Schermann G, Dobrev N, Sinning I, Fischer T. Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair. Cell. 2016;167:1001-1013.e7 pubmed publisher
chromatin immunoprecipitation; rat	Tran P, Kennedy B, Lien Y, Simmons R, Georgieff M. Fetal iron deficiency induces chromatin remodeling at the Bdnf locus in adult rat hippocampus. Am J Physiol Regul Integr Comp Physiol. 2015;308:R276-82 pubmed publisher
	Depierre D, Perrois C, Schickele N, Lhoumaud P, Abdi Galab M, Fosseprez O, et al. Chromatin in 3D distinguishes dMes-4/NSD and Hypb/dSet2 in protecting genes from H3K27me3 silencing. Life Sci Alliance. 2023;6: pubmed publisher
	Pancholi A, Klingberg T, Zhang W, Prizak R, Mamontova I, Noa A, et al. RNA polymerase II clusters form in line with surface condensation on regulatory chromatin. Mol Syst Biol. 2021;17:e10272 pubmed publisher
	Zhou H, Møhlenberg M, Terczyńska Dyla E, Winther K, Hansen N, Vad Nielsen J, et al. The IFNL4 Gene Is a Noncanonical Interferon Gene with a Unique but Evolutionarily Conserved Regulation. J Virol. 2020;94: pubmed publisher
	Calvo O, Grandin N, Jordán Pla A, Miñambres E, González Polo N, Pérez Ortín J, et al. The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription. Nucleic Acids Res. 2019;47:6250-6268 pubmed publisher
	Jiang J, Ma J, Liu B, Wang Y. Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research. Viruses. 2019;11: pubmed publisher
	Chen L, Lin Y, Gallegos D, Hazlett M, Gómez Schiavon M, Yang M, et al. Enhancer Histone Acetylation Modulates Transcriptional Bursting Dynamics of Neuronal Activity-Inducible Genes. Cell Rep. 2019;26:1174-1188.e5 pubmed publisher
	Touat Todeschini L, Shichino Y, Dangin M, Thierry Mieg N, Gilquin B, Hiriart E, et al. Selective termination of lncRNA transcription promotes heterochromatin silencing and cell differentiation. EMBO J. 2017;36:2626-2641 pubmed publisher
	Timblin G, Xie L, Tjian R, Schlissel M. Dual Mechanism of Rag Gene Repression by c-Myb during Pre-B Cell Proliferation. Mol Cell Biol. 2017;37: pubmed publisher
	Shadle S, Zhong J, Campbell A, Conerly M, Jagannathan S, Wong C, et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet. 2017;13:e1006658 pubmed publisher

product information

Product Type :

Antibody

Product Name :

POLR2A Monoclonal Antibody (8WG16)

Catalog # :

MA1-26249

Quantity :

100 ug

Price :

US 480.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Plant

Applications :

ChIP Assay: Assay-dependent, Immunocytochemistry: 1:100, Immunoprecipitation: Assay-dependent, Inhibition Assays: Assay-dependent, Western Blot: 1:1,000

Species :

Human, Mouse, Plant

Clone :

8WG16

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

DNA-directed RNA polymerase II subunit RPB1 (POLR1A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR1A is the largest subunit and is a catalytic component of RNA polymerase II which synthesizes mRNA precurors and many functional non-coding RNAs. It also forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft, and the jaws that are though to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic stie and is through to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II's largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levelts of the CTD-lysines.

Immunogen :

Purified wheat germ RNA polymerase II.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: Assay-dependent, Immunocytochemistry: 1:100, Immunoprecipitation: Assay-dependent, Inhibition Assays: Assay-dependent, Western Blot: 1:1,000

Aliases :

220kDa; DNA polymerase epsilon catalytic subunit; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directeced RNA polymerase II polypeptide A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DUN2; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; N0825; Pol II S2p; POL2; POLR2; Polr2a; POLRA; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8; YNL262W

more info or order :

Invitrogen product webpage