XPG Monoclonal Antibody (8H7) | Invitrogen MA1-26186 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

XPG Monoclonal Antibody (8H7)

catalog :

MA1-26186

quantity :

100 ug

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8H7

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:400; fig 2b	van Cuijk L, van Belle G, Turkyilmaz Y, Poulsen S, Janssens R, Theil A, et al. SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair. Nat Commun. 2015;6:7499 pubmed publisher
immunocytochemistry; human	Zhu Q, Wani G, Sharma N, Wani A. Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair. DNA Repair (Amst). 2012;11:942-50 pubmed publisher
western blot; human	Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed publisher
western blot; human	Uboldi S, Bernasconi S, Romano M, Marchini S, Fuso Nerini I, Damia G, et al. Characterization of a new trabectedin-resistant myxoid liposarcoma cell line that shows collateral sensitivity to methylating agents. Int J Cancer. 2012;131:59-69 pubmed publisher
immunocytochemistry; human	Arab H, Wani G, Ray A, Shah Z, Zhu Q, Wani A. Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state. PLoS ONE. 2010;5:e11007 pubmed publisher
western blot; human	Cazzalini O, Perucca P, Savio M, Necchi D, Bianchi L, Stivala L, et al. Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair. Nucleic Acids Res. 2008;36:1713-22 pubmed publisher
western blot; human; 1:200	Rubio D, Garcia S, Paz M, de la Cueva T, Lopez Fernandez L, Lloyd A, et al. Molecular characterization of spontaneous mesenchymal stem cell transformation. PLoS ONE. 2008;3:e1398 pubmed publisher
immunocytochemistry; human; 1:2000	Zotter A, Luijsterburg M, Warmerdam D, Ibrahim S, Nigg A, van Cappellen W, et al. Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH. Mol Cell Biol. 2006;26:8868-79 pubmed
immunocytochemistry; human; 1:100	Perucca P, Cazzalini O, Mortusewicz O, Necchi D, Savio M, Nardo T, et al. Spatiotemporal dynamics of p21CDKN1A protein recruitment to DNA-damage sites and interaction with proliferating cell nuclear antigen. J Cell Sci. 2006;119:1517-27 pubmed

product information

Product Type :

Antibody

Product Name :

XPG Monoclonal Antibody (8H7)

Catalog # :

MA1-26186

Quantity :

100 ug

Price :

US 524.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Immunoprecipitation: 2 ug/mg protein lysate, Western Blot: 1:625-1:1250

Species :

Human

Clone :

8H7

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.

Immunogen :

Recombinant human XPG (Xeroderma Pigmentosum type G) protein, expressed in baculovirus.

Format :

Liquid

Applications w/Dilutions :

Immunoprecipitation: 2 ug/mg protein lysate, Western Blot: 1:625-1:1250

Aliases :

COFS 3; COFS3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; DNA repair protein complementing XP-G cells homolog; ERCC 5; ERCC excision repair 5, endonuclease; ERCC5; ERCC5-201; ERCM 2; ERCM2; excision repair cross-complementation group 5; excision repair cross-complementing rodent repair deficiency, complementation group 5; OTTHUMP00000064902; RP11-484I6.5; UVDR; Xe; xeroderma pigmentosum; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum group G-complementing protein homolog; xeroderma pigmentosum, complementation group G; XPG; XPGC; XPG-complementing protein

more info or order :

Invitrogen product webpage