EGFR Monoclonal Antibody (F4) | Invitrogen MA1-24226 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Monoclonal Antibody (F4)

catalog :

MA1-24226

quantity :

100 µg

price :

US 475

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:50	Baek K, Hong M, Jung Y, Lee C, Lee T, Park E, et al. Correlation of AR, EGFR, and HER2 Expression Levels in Prostate Cancer: Immunohistochemical Analysis and Chromogenic In Situ Hybridization. Cancer Res Treat. 2012;44:50-6 pubmed publisher
immunohistochemistry; human	Linder N, Konsti J, Turkki R, Rahtu E, Lundin M, Nordling S, et al. Identification of tumor epithelium and stroma in tissue microarrays using texture analysis. Diagn Pathol. 2012;7:22 pubmed publisher
immunohistochemistry; human	Mackiewicz M, Huppi K, Pitt J, Dorsey T, Ambs S, Caplen N. Identification of the receptor tyrosine kinase AXL in breast cancer as a target for the human miR-34a microRNA. Breast Cancer Res Treat. 2011;130:663-79 pubmed publisher
immunohistochemistry; human	Glynn S, Boersma B, Dorsey T, Yi M, Yfantis H, Ridnour L, et al. Increased NOS2 predicts poor survival in estrogen receptor-negative breast cancer patients. J Clin Invest. 2010;120:3843-54 pubmed publisher
immunohistochemistry; human	Martin D, Boersma B, Yi M, Reimers M, Howe T, Yfantis H, et al. Differences in the tumor microenvironment between African-American and European-American breast cancer patients. PLoS ONE. 2009;4:e4531 pubmed publisher
immunohistochemistry; human; 1:100	Li H, Zhou G, Fu X, Zhang L. Antigen expression of human eccrine sweat glands. J Cutan Pathol. 2009;36:318-24 pubmed publisher
immunohistochemistry; human	Schiavo R, Tullio C, La Grotteria M, Andreotti I, Scarpati B, Romiti L, et al. Establishment and characterization of a new Ewing's sarcoma cell line from a malignant pleural effusion. Anticancer Res. 2007;27:3273-8 pubmed
immunohistochemistry; human; 1:50	Jin J, Wu C, Lin Y, Wang J, Yu C, Sheu L, et al. Higher expression of epidermal growth factor receptor is associated with extracellular matrix metalloprotease inducer in colorectal adenocarcinoma: tissue microarray analysis of immunostaining score with clinicopathological parameters. Dis Markers. 2006;22:309-16 pubmed
immunohistochemistry; human	Wang S, Nakajima Y, Yu Y, Xia W, Chen C, Yang C, et al. Tyrosine phosphorylation controls PCNA function through protein stability. Nat Cell Biol. 2006;8:1359-68 pubmed
immunohistochemistry; human; 1:50	Avninder S, Sharma M, Deb P, Mehta V, Karak A, Mahapatra A, et al. Gemistocytic astrocytomas: histomorphology, proliferative potential and genetic alterations--a study of 32 cases. J Neurooncol. 2006;78:123-7 pubmed
immunohistochemistry; human; 1:50	Rayala S, Hollander P, Balasenthil S, Molli P, Bean A, Vadlamudi R, et al. Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK. J Biol Chem. 2006;281:4395-403 pubmed
western blot; human	Pangburn H, Kraus H, Ahnen D, Rice P. Sulindac metabolites inhibit epidermal growth factor receptor activation and expression. J Carcinog. 2005;4:16 pubmed
	Hajdu T, Váradi T, Rebenku I, Kovacs T, Szollosi J, Nagy P. Comprehensive Model for Epidermal Growth Factor Receptor Ligand Binding Involving Conformational States of the Extracellular and the Kinase Domains. Front Cell Dev Biol. 2020;8:776 pubmed publisher
	Tanimura K, Nakago S, Murakoshi H, Takekida S, Moriyama T, Matsuo H, et al. Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy. Eur J Endocrinol. 2004;151:93-101 pubmed
	Chen Z, de Paiva C, Luo L, Kretzer F, Pflugfelder S, Li D. Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells. 2004;22:355-66 pubmed
	Matalliotakis I, Goumenou A, Koumantakis G, Athanassakis I, Dionyssopoulou E, Neonaki M, et al. Expression of serum human leukocyte antigen and growth factor levels in a Greek family with familial endometriosis. J Soc Gynecol Investig. 2003;10:118-21 pubmed
	Matalliotakis I, Goumenou A, Koumantakis G, Neonaki M, Koumantakis E, Dionyssopoulou E, et al. Serum concentrations of growth factors in women with and without endometriosis: the action of anti-endometriosis medicines. Int Immunopharmacol. 2003;3:81-9 pubmed

product information

Product Type :

Antibody

Product Name :

EGFR Monoclonal Antibody (F4)

Catalog # :

MA1-24226

Quantity :

100 µg

Price :

US 475

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Radioimmune assays: Assay-dependent, Western Blot: 1 µg/mL

Species :

Human

Clone :

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

Synthetic peptide corresponding to residues D(985) V V D A D E Y L I P Q(996) of human EGFR.

Format :

Liquid

Applications w/Dilutions :

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2