MDM2 Monoclonal Antibody (SMP14) | Invitrogen MA1-23318 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MDM2 Monoclonal Antibody (SMP14)

catalog :

MA1-23318

quantity :

50µg

price :

US 375.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SMP14

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200	Phesse T, Myant K, Cole A, Ridgway R, Pearson H, Muncan V, et al. Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo. Cell Death Differ. 2014;21:956-66 pubmed publisher
immunohistochemistry; human	Kusafuka K, Miki T, Nakajima T. Salivary adenoid cystic carcinoma with an early phase of high-grade transformation: case report with an immunohistochemical analysis. Diagn Pathol. 2013;8:113 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Jung Y, Joo K, Seong D, Choi Y, Kong D, Kim Y, et al. Identification of prognostic biomarkers for glioblastomas using protein expression profiling. Int J Oncol. 2012;40:1122-32 pubmed publisher
western blot; human	Li C, Cheng Y, Liao P, Kang J. Translocation of p53 to mitochondria is regulated by its lipid binding property to anionic phospholipids and it participates in cell death control. Neoplasia. 2010;12:150-60 pubmed
western blot; human	Hwang Y, Lu T, Huang D, Kuo Y, Kao C, Yeh N, et al. NOLC1, an enhancer of nasopharyngeal carcinoma progression, is essential for TP53 to regulate MDM2 expression. Am J Pathol. 2009;175:342-54 pubmed publisher
immunohistochemistry; human; 1:10	Martinez A, Brethauer U, Borlando J, Spencer M, Rojas I. Epithelial expression of p53, mdm-2 and p21 in normal lip and actinic cheilitis. Oral Oncol. 2008;44:878-83 pubmed publisher
immunohistochemistry; human; 1:100	Das P, Vaiphei K, Jain D, Wig J. p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters. Int J Surg Pathol. 2007;15:335-45 pubmed
immunohistochemistry; human; 1:300	Melck A, Masoudi H, Griffith O, Rajput A, Wilkins G, Bugis S, et al. Cell cycle regulators show diagnostic and prognostic utility for differentiated thyroid cancer. Ann Surg Oncol. 2007;14:3403-11 pubmed
immunohistochemistry; human; 1:40	Tzouvala M, Lazaris A, Papatheodoridis G, Kouvidou C, Papathomas T, Kavantzas N, et al. Potential role of apoptosis and apoptotic regulatory proteins in colorectal neoplasia: correlations with clinico-pathological parameters and survival. Dig Dis Sci. 2008;53:451-60 pubmed
immunohistochemistry; human; 1:200	Agaimy A, Wünsch P, Schroeder J, Gaumann A, Dietmaier W, Hartmann A, et al. Low-grade abdominopelvic sarcoma with myofibroblastic features (low-grade myofibroblastic sarcoma): clinicopathological, immunohistochemical, molecular genetic and ultrastructural study of two cases with literature review. J Clin Pathol. 2008;61:301-6 pubmed
immunocytochemistry; human	Lindström M, Jin A, Deisenroth C, White Wolf G, Zhang Y. Cancer-associated mutations in the MDM2 zinc finger domain disrupt ribosomal protein interaction and attenuate MDM2-induced p53 degradation. Mol Cell Biol. 2007;27:1056-68 pubmed
immunohistochemistry - paraffin section; human; 1:50; fig 1	Cho E, Choi Y, Chae S, Sohn J, Ahn G. Relationship between p53-associated proteins and estrogen receptor status in ovarian serous neoplasms. Int J Gynecol Cancer. 2006;16:1000-6 pubmed
immunohistochemistry; human; 1:200	Turbin D, Cheang M, Bajdik C, Gelmon K, Yorida E, De Luca A, et al. MDM2 protein expression is a negative prognostic marker in breast carcinoma. Mod Pathol. 2006;19:69-74 pubmed
immunohistochemistry - paraffin section; human immunohistochemistry; human	Ricciardiello L, Ceccarelli C, Angiolini G, Pariali M, Chieco P, Paterini P, et al. High thymidylate synthase expression in colorectal cancer with microsatellite instability: implications for chemotherapeutic strategies. Clin Cancer Res. 2005;11:4234-40 pubmed
western blot; human	Rahman R, Latonen L, Wiman K. hTERT antagonizes p53-induced apoptosis independently of telomerase activity. Oncogene. 2005;24:1320-7 pubmed
	Qiu L, Sinniah R, Hsu S. Coupled induction of iNOS and p53 upregulation in renal resident cells may be linked with apoptotic activity in the pathogenesis of progressive IgA nephropathy. J Am Soc Nephrol. 2004;15:2066-78 pubmed
	Zhang Y, Wolf G, Bhat K, Jin A, Allio T, Burkhart W, et al. Ribosomal protein L11 negatively regulates oncoprotein MDM2 and mediates a p53-dependent ribosomal-stress checkpoint pathway. Mol Cell Biol. 2003;23:8902-12 pubmed
	Hann B, Balmain A. Replication of an E1B 55-kilodalton protein-deficient adenovirus (ONYX-015) is restored by gain-of-function rather than loss-of-function p53 mutants. J Virol. 2003;77:11588-95 pubmed
	Hashemi J, Lindström M, Asker C, Platz A, Hansson J, Wiman K. A melanoma-predisposing germline CDKN2A mutation with functional significance for both p16 and p14ARF. Cancer Lett. 2002;180:211-21 pubmed
	Paradiso A, Ranieri G, Simone G, Silvestris N, Costa A, De Lena M, et al. mdm2-p53 Interaction: lack of correlation with the response to 5-fluorouracil in advanced colorectal cancer. Oncology. 2002;62:278-85 pubmed
	Kersemaekers A, Mayer F, Molier M, Van Weeren P, Oosterhuis J, Bokemeyer C, et al. Role of P53 and MDM2 in treatment response of human germ cell tumors. J Clin Oncol. 2002;20:1551-61 pubmed
	Noffsinger A, Belli J, Miller M, Fenoglio Preiser C. A unique basal pattern of p53 expression in ulcerative colitis is associated with mutation in the p53 gene. Histopathology. 2001;39:482-92 pubmed
	Zhang Y, Xiong Y. Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53. Mol Cell. 1999;3:579-91 pubmed
	Dei Tos A, Piccinin S, Doglioni C, Vukosavljevic T, Mentzel T, Boiocchi M, et al. Molecular aberrations of the G1-S checkpoint in myxoid and round cell liposarcoma. Am J Pathol. 1997;151:1531-9 pubmed

product information

Product Type :

Antibody

Product Name :

MDM2 Monoclonal Antibody (SMP14)

Catalog # :

MA1-23318

Quantity :

50µg

Price :

US 375.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Frozen): 1:200-1:500, Immunohistochemistry (Paraffin): 1:200-1:500, Immunoprecipitation: Assay dependent

Species :

Human

Clone :

SMP14

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Clone SMP14 recognizes the 90kDa MDM2 protein. MDM2 forms a complex with p53 which leads to inhibition of p53 function. MDM2 has also been shown to stimulate cellular proliferation via induction of transcriptional regulators including E2F1 and DP1. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer.

Immunogen :

Synthetic peptide corresponding to amino acids 154-167 (CSRPSTSSRRRAISE) of human MDM2.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): 1:200-1:500, Immunohistochemistry (Paraffin): 1:200-1:500, Immunoprecipitation: Assay dependent

Aliases :

HDMX, MGC5370, MGC71221, hdm2