BRCA1 Monoclonal Antibody (6B4) | Invitrogen MA1-23164 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

BRCA1 Monoclonal Antibody (6B4)

catalog :

MA1-23164

quantity :

100 µL

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6B4

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation, immunocytochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:400; fig 6 western blot; human; 1:1000; fig 5 immunocytochemistry knockout validation; mouse; 1:400; fig 6 western blot knockout validation; mouse; 1:1000; fig 5	Kargaran P, Yasaei H, Anjomani Virmouni S, Mangiapane G, Slijepcevic P. Analysis of alternative lengthening of telomere markers in BRCA1 defective cells. Genes Chromosomes Cancer. 2016;55:864-76 pubmed publisher
	Steffens Reinhardt L, Zhang X, Groen K, Morten B, De Iuliis G, Braithwaite A, et al. Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage. Cell Death Dis. 2022;13:907 pubmed publisher
	Mehta K, Laimins L. Human Papillomaviruses Preferentially Recruit DNA Repair Factors to Viral Genomes for Rapid Repair and Amplification. MBio. 2018;9: pubmed publisher

product information

Product Type :

Antibody

Product Name :

BRCA1 Monoclonal Antibody (6B4)

Catalog # :

MA1-23164

Quantity :

100 µL

Price :

US 450.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ChIP Assay: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:3,000

Species :

Human, Mouse

Clone :

6B4

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Immunogen :

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:3,000

Aliases :

brca 1; Brca1; brca-1; BRCA1, DNA repair associated; BRCA1/BRCA2-containing complex, subunit 1; BRCAI; BRCC1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; breast cancer associated 1; breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; BROVCA1; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; FANCS; IRIS; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; PNCA4; PPP1R53; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; RNF53; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein

more info or order :

Invitrogen product webpage