Sarcomeric alpha Actinin Monoclonal Antibody (EA-53) | Invitrogen MA1-22863 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Sarcomeric alpha Actinin Monoclonal Antibody (EA-53)

catalog :

MA1-22863

quantity :

500 µL

price :

US 474

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EA-53

reactivity :

human, mouse, pigs , zebrafish , domestic rabbit

application :

immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:25; loading ...; fig 7s1c	Selvaraj S, Mondragón González R, Xu B, Magli A, Kim H, Laine J, et al. Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes. elife. 2019;8: pubmed publisher
immunohistochemistry; human; 1:500; fig 1a	Pointon A, Pilling J, Dorval T, Wang Y, Archer C, Pollard C. From the Cover: High-Throughput Imaging of Cardiac Microtissues for the Assessment of Cardiac Contraction during Drug Discovery. Toxicol Sci. 2017;155:444-457 pubmed publisher
immunohistochemistry; zebrafish ; 1:500; fig 2a	Zou J, Tran D, Baalbaki M, Tang L, Poon A, Pelonero A, et al. An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish. elife. 2015;4:e09406 pubmed publisher
immunocytochemistry; mouse; 1:400; fig 3	Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
immunocytochemistry; pigs ; 1:100 immunohistochemistry; pigs	Shapiro S, Ranjan A, Kawase Y, Cheng R, Kara R, Bhattacharya R, et al. Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes. Sci Transl Med. 2014;6:224ra27 pubmed publisher
immunohistochemistry; human	Guan X, Mack D, Moreno C, Strande J, Mathieu J, Shi Y, et al. Dystrophin-deficient cardiomyocytes derived from human urine: new biologic reagents for drug discovery. Stem Cell Res. 2014;12:467-80 pubmed publisher
immunocytochemistry; human	Maioli M, Contini G, Santaniello S, Bandiera P, Pigliaru G, Sanna R, et al. Amniotic fluid stem cells morph into a cardiovascular lineage: analysis of a chemically induced cardiac and vascular commitment. Drug Des Devel Ther. 2013;7:1063-73 pubmed publisher
	Ng W, Johnston E, Tan J, Bliley J, Feinberg A, Stolz D, et al. Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells. elife. 2022;11: pubmed publisher
	Simmons T, Vetter T, Huang N, Vulin Chaffiol A, Wein N, Flanigan K. Pre-clinical dose-escalation studies establish a therapeutic range for U7snRNA-mediated DMD exon 2 skipping. Mol Ther Methods Clin Dev. 2021;21:325-340 pubmed publisher
	Jensen J, Cakal S, Li J, Pless C, Radeke C, Jepsen M, et al. Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates. Sci Rep. 2020;10:13305 pubmed publisher
	Filippo Buono M, von Boehmer L, Strang J, Hoerstrup S, Emmert M, Nugraha B. Human Cardiac Organoids for Modeling Genetic Cardiomyopathy. Cells. 2020;9: pubmed publisher
	Kobayashi K, Ichihara Y, Sato N, Umeda N, Fields L, Fukumitsu M, et al. On-site fabrication of Bi-layered adhesive mesenchymal stromal cell-dressings for the treatment of heart failure. Biomaterials. 2019;209:41-53 pubmed publisher
	Yang C, Xu Y, Yu M, Lee D, Alharti S, Hellen N, et al. Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis. Hum Mol Genet. 2017;26:3031-3045 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Sarcomeric alpha Actinin Monoclonal Antibody (EA-53)

Catalog # :

MA1-22863

Quantity :

500 µL

Pricing :

US 474

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit, Zebrafish

Applications :

Immunocytochemistry: Assay-dependent, Immunohistochemistry - Free Floating: Assay-dependent, Immunohistochemistry (Paraffin): 1:20-1:30

Species :

Human, Mouse, Rabbit, Zebrafish

Clone :

EA-53

Isotype :

IgG1

Storage :

4° C

Description :

This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

Immunogen :

Purified rabbit skeletal alpha-actinin.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunohistochemistry - Free Floating: Assay-dependent, Immunohistochemistry (Paraffin): 1:20-1:30

Aliases :

3110023F10Rik; actinin 1 smooth muscle; actinin alpha 1; actinin, alpha 1; ACTN1; Actn1a; alpha actinin 1a; alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; F-actin cross-linking protein; non-muscle alpha-actinin 1; non-muscle alpha-actinin-1

more info or order :

Invitrogen product webpage